The role of endogenous miRNAs in the development of cerebral aneurysms

Gareev, Ilgiz; Safin, Shamil M.

doi:10.17116/neiro201983011112

Cited by 4 publications

(2 citation statements)

References 51 publications

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“…MicroRNAs (miRNAs) are endogenous non-coding single-stranded small RNAs with a length of 19-22 nucleotides, which are widely present in the biological world (10). MiRNAs regulate the expression of target genes by fully or partially complementary binding to the 3'UTR regions of target mRNAs (11) and participate in different physiological and pathological processes (12,13). Increasing numbers of studies have demonstrated that miRNAs play crucial roles in intracerebral hemorrhage by regulating various kinds of progress (14).…”

Section: Introductionmentioning

confidence: 99%

MiR-20a-5p targets RBM24 and alleviates hypertensive intracerebral hemorrhage

Li Xu,

Chuangqi Mo,

Ming Lu

et al. 2023

Cell Mol Biol (Noisy-le-grand)

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Section: Introductionmentioning

confidence: 99%

MiR-20a-5p targets RBM24 and alleviates hypertensive intracerebral hemorrhage

Li Xu,

Chuangqi Mo,

Ming Lu

et al. 2023

Cell Mol Biol (Noisy-le-grand)

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“…MicroRNAs (microRNAs, miRNAs) are endogenous non-coding single-stranded small RNAs with a length of 19-22 nucleotides, which are widely present in the biological world [11]. miRNAs regulate the expression of target genes by fully or partially complementary binding to the 3'UTR region of target mRNA [12] and participate in different physiological and pathological processes [13,14]. Increasing numbers of studies have demonstrated that miRNAs play crucial roles in intracerebral hemorrhage by regulating various kinds of progresses [15].…”

Section: Introductionmentioning

confidence: 99%

miR-20a-5p/RBM24 Axis Alleviated Hypertensive Intracerebral Hemorrhage via Regulating HIF1α/VEGFA Signaling Pathway

Xu¹,

Mo²,

Lü³

et al. 2021

Preprint

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Background: Hypertensive intracerebral hemorrhage presented high incidence and high mortality owing to its difficult to diagnose. However, the molecular mechanism of HICH remains unclear. Therefore, this study aims to investigate the key miRNAs and the mechanism of the key miRNAs in HICH. Methods: miRNAs chip was used to explore the differentially expressed miRNAs in HICH patients. In vitro and in vivo HICH models were established by Ang-II. Cell Counting Kit-8 (CCK8), flow cytometry, transwell assay and tube formation analysis were used to detect cell proliferation, apoptosis, cell migration and tube formation, respectively. Hematoxylin-eosin staining was used to evaluate the intracerebral hemorrhage in vivo HICH model. The regulatory mechanism of miR-20a-5p in HICH was confirmed by dual luciferase reporter assay, immunofluorescence, qRT-PCR, western blot and rescue experiments. Results: miR-20a-5p showed the most downregulated in HICH patients compared with healthy individuals and significantly associated with clinicopathological characteristics of HICH. Upregulation of miR-20a-5p promoted cell proliferation, migration and tube formation while inhibited apoptosis in vitro and ameliorated the development of HICH in vivo. RBM24 is a direct target of miR-20a-5p and silencing RBM24 could partially recovery the development of HICH caused by miR-20a-5p inhibition both in vivo and in vitro. miR-20a-5p regulated the development of HICH depending on HIF1α/VEGFA pathway. Conclusion: Our results demonstrated that miR-20a-5p/RBM24 axis regulated hypertensive intracerebral hemorrhage via regulating HIF1α/VEGFA signaling pathway, in support of further investigation into miR-20a-5p therapies for HICH treatment.

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