The role of endoplasmic reticulum stress in endometriosis

Al-Hetty, Hussein Riyadh Abdul Kareem; Jabbar, Ahmed D.; Vf, Eremin; Jabbar, Abeer Mohsen; Jalil, Abduladheem Turki; Al-Dulimi, Ali G.; Gharban, Hasanain A. J.; Khan, Muhammad Usman; Saleh, Marwan Mahmood

doi:10.1007/s12192-023-01323-2

Cited by 5 publications

(1 citation statement)

References 45 publications

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“…Although significant efforts have been dedicated to revealing the exact pathogenesis of endometriosis, a unified, convincing conclusion has not yet been reached. A recently published literature review shows that numerous studies have focused on the important role of ER stress in endometriosis ( Al-Hetty et al, 2023 ). In our study, we successfully constructed an ER stress-related signature, which performed well in discriminating endometriosis from normal samples.…”

Section: Discussionmentioning

confidence: 99%

Identification and validation of an endoplasmic-reticulum-stress-related gene signature as an effective diagnostic marker of endometriosis

Wang,

Ji,

Sun

2024

PeerJ

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Background Endometriosis is one of the most common benign gynecological diseases and is characterized by chronic pain and infertility. Endoplasmic reticulum (ER) stress is a cellular adaptive response that plays a pivotal role in many cellular processes, including malignant transformation. However, whether ER stress is involved in endometriosis remains largely unknown. Here, we aimed to explore the potential role of ER stress in endometriosis, as well as its diagnostic value. Methods We retrieved data from the Gene Expression Omnibus (GEO) database. Data from the GSE7305 and GSE23339 datasets were integrated into a merged dataset as the training cohort. Differentially expressed ER stress-related genes (DEG-ERs) were identified by integrating ER stress-related gene profiles downloaded from the GeneCards database with differentially expressed genes (DEGs) in the training cohort. Next, an ER stress-related gene signature was identified using LASSO regression analysis. The receiver operating characteristic curve was used to evaluate the discriminatory ability of the constructed model, which was further validated in the GSE51981 and GSE105764 datasets. Online databases were used to explore the possible regulatory mechanisms of the genes in the signature. Meanwhile, the CIBERSORT algorithm and Pearson correlation test were applied to analyze the association between the gene signature and immune infiltration. Finally, expression levels of the signature genes were further detected in clinical specimens using qRT-PCR and validated in the Turku endometriosis database. Results In total, 48 DEG-ERs were identified in the training cohort. Based on LASSO regression analysis, an eight-gene-based ER stress-related gene signature was constructed. This signature exhibited excellent diagnostic value in predicting endometriosis. Further analysis indicated that this signature was associated with a compromised ER stress state. In total, 12 miRNAs and 23 lncRNAs were identified that potentially regulate the expression of ESR1, PTGIS, HMOX1, and RSAD2. In addition, the ER stress-related gene signature indicated an immunosuppressive state in endometriosis. Finally, all eight genes showed consistent expression trends in both clinical samples and the Turku database compared with the training dataset. Conclusions Our work not only provides new insights into the impact of ER stress in endometriosis but also provides a novel biomarker with high clinical value.

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Section: Discussionmentioning

confidence: 99%

Identification and validation of an endoplasmic-reticulum-stress-related gene signature as an effective diagnostic marker of endometriosis

Wang,

Ji,

Sun

2024

PeerJ

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Effect of astaxanthin supplementation on female fertility and reproductive outcomes: a systematic review and meta-analysis of clinical and animal studies

Maleki-Hajiagha,

Shafie,

Maajani

et al. 2024

J Ovarian Res

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Context Oxidative stress (OS) plays a harmful role in female reproduction and fertility. Several studies explored various dietary interventions and antioxidant supplements, such as astaxanthin (AST), to mitigate the adverse effects of OS on female fertility. Ameliorative effects of AST on female fertility and the redox status of reproductive organs have been shown in several animal and clinical studies. Objectives The main objective of present systematic review and meta-analysis of both animal and clinical studies was to provide a comprehensive overview of the current evidence on the effects of AST on female fertility and reproductive outcomes. The effect of AST on redox status, inflammatory and apoptotic markers in reproductive organs were included as the secondary outcomes. Data sources We systematically searched electronic databases including PubMed, Scopus, and Web of Science, until January 1, 2024, using specified search terms related to AST, female reproductive performance, and infertility, considering the diverse synonyms found in the literature for interventional studies that compared oral AST supplementation with placebo or control in human or animal models. Data extraction Two independent reviewers extracted data on study characteristics, outcomes, and risk of bias. We pooled the results using random-effects models and assessed the heterogeneity and quality of evidence. We descriptively reported the data from animal models, as meta-analysis was not possible. Data analysis The meta-analysis of clinical trials showed that AST significantly increased the oocyte maturation rate (MD: 8.40, 95% CI: 4.57 to 12.23, I2: 0%) and the total antioxidant capacity levels in the follicular fluid (MD: 0.04, 95% CI: 0.02 to 0.06, I2: 0%). The other ART and pregnancy outcomes and redox status markers did not show statistically significant changes. The animal studies reported ameliorative effects of AST on redox status, inflammation, apoptosis, and ovarian tissue histomorphology. Conclusion This systematic review shows that AST supplementation may improve assisted reproductive technology outcomes by enhancing oocyte quality and reducing OS in the reproductive organs. However, the evidence is limited by the heterogeneity, risk of bias, and small sample size of the included studies. Graphical Abstract

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Research progress on the beneficial effects of exercise on endocrine system-related diseases in women by regulating ER stress pathways

Chen,

Zhang,

Sheng

et al. 2023

Preprint

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Exercise, as an effective non-drug intervention, plays an important role in preventing and alleviating several diseases. Endoplasmic reticulum (ER) stress is caused by an excessive accumulation of unfolded or misfolded proteins in the ER and also serves as the body’s internal self-protection mechanism. ER stress occurrence can be detected in the cells in many diseases such as cancer, diabetes, obesity, osteoporosis, neurodegenerative diseases, and metabolic diseases. In recent years, exercise has been suggested to change the molecular mechanisms related to various diseases by regulating ER stress. With increasing attention on women's health, some common diseases have also become research hotspots, such as breast, ovarian, cervical, endometrial cancer, polycystic ovary syndrome (PCOS) and endometriosis prevention and treatment; and other diseases. This manuscript reviews the relationship between exercise and ER stress and its role in common female endocrine system-related diseases.

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The role of endoplasmic reticulum stress in endometriosis

Cited by 5 publications

References 45 publications

Identification and validation of an endoplasmic-reticulum-stress-related gene signature as an effective diagnostic marker of endometriosis

Identification and validation of an endoplasmic-reticulum-stress-related gene signature as an effective diagnostic marker of endometriosis

Effect of astaxanthin supplementation on female fertility and reproductive outcomes: a systematic review and meta-analysis of clinical and animal studies

Research progress on the beneficial effects of exercise on endocrine system-related diseases in women by regulating ER stress pathways

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