The role of environmental factors for the composition of microbial communities of saline lakes in the Novosibirsk region (Russia)

Bryanskaya, A. V.; Malup, T. K.; Лазарева, Е. В.; Taran, Oxana P.; Розанов, А. С.; Ефимов, В. М.; Peltek, S. E.

doi:10.1186/s12866-015-0618-y

Cited by 29 publications

(20 citation statements)

References 48 publications

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“…In water and microbial lake mate, Cyanobacteria and Proteobacteria dominated. It is established that the ecosystem of the Salt Lake is characterized by: (1) variations in geochemical parameters; (2) developing mass of microbial community with prevalence of cyanobacteria; (3) the presence of powerful layers of bottom sediments with a high taxonomic diversity of prokaryotes; (4) intensive development of the Artemia crab; (5) tracking changes in biological and geochemical characteristics in dynamics and extrapolating the data obtained to other lake ecosystems in the Novosibirsk region, characterized by a similar geochemical composition of natural solutions [1,2].…”

Section: Motivation and Aimmentioning

confidence: 99%

Study of bioresources of salt lakes of Novosibirsk oblast

2018

11-Ая Международная Конференция По Биоинформатике Регуляции И Структуры Геномов И Системной Биологии

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Motivation and Aim:In the Kulunda and Baraba Steppes there are many brackish and saline lakes. These lakes are unstable water systems, characterized by significant fluctuations in the water level so the microorganism communities developing in them have various adaptations that allow them to withstand significant fluctuations in environmental factors. The aim of this study was to investigate the fundamentals of biogeochemistry and microbiology of extreme environments using small saline drainless lakes of the Novosibirsk oblast. Methods and Algorithms: As a model object we took the Solenoye Lake (Novosibirsk oblast). We studied the composition of the benthic microbial community using parallel amplicon sequencing of the V3 variable region of the 16S rRNA gene, as well as geochemical parameters (pH, Eh, cationic/anionic composition, gas components). We described and compared different layers of the bottom sediments and found relations between chemical composition and microbial content of the bottom sediments. Results: As a result, it has been established that geochemical data on the study of the structure of the microbial community are well correlated. The upper 30-35 cm of the sediment by the ratio of the main groups of microorganisms are somewhat similar, and below, where the change occurs and the main geochemical and mineralogical indices of the substance, the composition of the community changes significantly. Throughout the sediment column, Actinobacteria and/or Proteobacteria predominated. In the layers of the columns the number of prokaryotic sequences found was from 96 to 414. The proportion of archaeal sequences in the bottom sediments was 5.5-6.6 %, in water and microbial mathematics it was 2.0 and 1.2 %. In the lake water 50 prokaryotic sequences were identified, in the microbial mate -81, in the columns of bottom sediments -390.

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Section: Motivation and Aimmentioning

confidence: 99%

Study of bioresources of salt lakes of Novosibirsk oblast

2018

11-Ая Международная Конференция По Биоинформатике Регуляции И Структуры Геномов И Системной Биологии

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“…Так, растворы озер Новосибирской обла-сти -нейтральные и слабощелочные (6.5 < pH < 8.5), ха-рактеризовались повышенной соленостью (12.3-26.7 г/л) либо относились к рассолам с минерализацией от 100 до 290 г/л (Bryanskaya et al, 2016). Растворы озер содержали также значительные концентрации B, Li, Sr, I (Лазарева и др., 2012).…”

Section: материалы и методыunclassified

Collection of microorganisms of ICG SB RAS as a genetic resource for biotechnology

Брянская¹,

Уварова²,

Розанов³

et al. 2017

Vestn. VOGiS

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Genetic knowledge of microorganisms plays a critical role in the creation of new biotechnologies, since the effectiveness of any biotechnology is determined by the particular qualities of the structurally functional organization of molecular-genetic systems and their components used for the production of targeted products. Collections of microbial cultures play a decisive role in mobilizing biological resources and make it possible to form a solid base for genetic, molecular biological and biotechnological research. The aim of this work was to assess the key molecular-genetic and phenotypic characteristics of strains of the collection of microorganisms created in the "FRC Institute of Cytology and Genetics of the Siberian Branch of the Russian Academy of Sciences" as a genetic resource for biotechnology. Thirty strains of microorganisms of the collection were isolated by employees of the FRC ICG SB RAS from extreme natural ecosystems, the key molecular-genetic and phenotypic characteristics were described using modern methods of molecular biology and mass-spectrometry. DNA isolation and the sequencing of 16S rRNA gene sequences were performed. The strains of the collection were characterized by morphological, physiological, moleculargenetic and mass-spectrometric characteristics. The particular qualities of growing of strains on different substrates have been established, the study of cell morphology has been carried out. The physiological characteristics of the strains of the collection have been established: the attitude to oxygen, the type of nutrition, the range of temperature and pH, the attitude to NaCl and others. Different resistance of strains to antibiotics has been established. The creation of personal mass spectra of protein profiles of the studied strains of the collection was carried out. The resulting DNA sequences of the strains are deposited in the GenBank. The chemotaxonomic characteristics В создании новых биотехнологий важное значение имеют генети-ческие знания о микроорганизмах, так как эффективность любых биотехнологий определяется особенностями структурно-функ-циональной организации молекулярно-генетических систем и их компонент, используемых для наработки целевых продуктов. При этом коллекции микробных культур играют решающую роль в мо-билизации биологических ресурсов и позволяют сформировать прочную базу для генетических, молекулярно-биологических и биотехнологических исследований. Целью данной работы было проведение оценки ключевых молекулярно-генетических и фено-типических характеристик штаммов коллекции микроорганизмов, созданной в Федеральном исследовательском центре Институт цитологии и генетики (ФИЦ ИЦиГ) СО РАН, в качестве генетическо-го ресурса для биотехнологии. Для 30 штаммов микроорганизмов коллекции, выделенных сотрудниками ИЦиГ из экстремальных природных экосистем, осуществлено описание ключевых молеку-лярно-генетических и фенотипических характеристик с использо-ванием современных методов молекулярной биологии и масс-спектрометрии. Проведено выделение ДНК и секвенирование по...

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“…Microbial communities in extreme environments have formed unique community structures ( Oren, 2013 ). Moreover, the species diversity and distribution patterns of microbial assemblages vary, together with dynamic ecological variables such as pH, geochemical elements, moisture and terrestrial locations ( Xiong et al, 2012 ; Liu K. et al, 2014 ; Bryanskaya et al, 2016 ; Fernandez et al, 2016 ; Zhong et al, 2016 ). Revealing the linkage between the diversity and structures of microbial communities and environmental driving forces will provide significant insight into the mechanisms that microbial communities use to adapt to extreme habitats, community succession and ecological functioning.…”

Section: Introductionmentioning

confidence: 99%

Macro and Microelements Drive Diversity and Composition of Prokaryotic and Fungal Communities in Hypersaline Sediments and Saline–Alkaline Soils

et al. 2018

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Understanding the effects of environmental factors on microbial communities is critical for microbial ecology, but it remains challenging. In this study, we examined the diversity (alpha diversity) and community compositions (beta diversity) of prokaryotes and fungi in hypersaline sediments and salinized soils from northern China. Environmental variables were highly correlated, but they differed significantly between the sediments and saline soils. The compositions of prokaryotic and fungal communities in the hypersaline sediments were different from those in adjacent saline–alkaline soils, indicating a habitat-specific microbial distribution pattern. The macroelements (S, P, K, Mg, and Fe) and Ca were, respectively, correlated closely with the alpha diversity of prokaryotes and fungi, while the macronutrients (e.g., Na, S, P, and Ca) were correlated with the prokaryotic and fungal beta-diversity (P ≤ 0.05). And, the nine microelements (e.g., Al, Ba, Co, Hg, and Mn) and micronutrients (Ba, Cd, and Sr) individually shaped the alpha diversity of prokaryotes and fungi, while the six microelements (e.g., As, Ba, Cr, and Ge) and only the trace elements (Cr and Cu), respectively, influenced the beta diversity of prokaryotes and fungi (P < 0.05). Variation-partitioning analysis (VPA) showed that environmental variables jointly explained 55.49% and 32.27% of the total variation for the prokaryotic and fungal communities, respectively. Together, our findings demonstrate that the diversity and community composition of the prokaryotes and fungi were driven by different macro and microelements in saline habitats, and that geochemical elements could more widely regulate the diversity and community composition of prokaryotes than these of fungi.

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The role of environmental factors for the composition of microbial communities of saline lakes in the Novosibirsk region (Russia)

Cited by 29 publications

References 48 publications

Study of bioresources of salt lakes of Novosibirsk oblast

Study of bioresources of salt lakes of Novosibirsk oblast

Collection of microorganisms of ICG SB RAS as a genetic resource for biotechnology

Macro and Microelements Drive Diversity and Composition of Prokaryotic and Fungal Communities in Hypersaline Sediments and Saline–Alkaline Soils

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