The Role of Estrogen and Progesterone Hormone on Vaginal Cytology in Bitch

Haji, Moon Moon; Ahmed, Faheem; Lalrintluanga, K.; Talukdar, Dibyajyoti; Doley, Probal Jyoti; Behera, Sujit Kumar; Sarma, Kalyan

doi:10.5455/ijlr.20171117025427

Cited by 5 publications

(4 citation statements)

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“…Then on the 5th day, no parabasal cells were seen in the vaginal examination, and superficial cells began to dominate. The results of this examination differ from the study conducted on clinically healthy adult female dogs by Haji et al (2018) [11] and Gupta et al (2012) [12] , which showed that the proportion of superficial cells was higher during proestrus. Differences in the percentage of cells in the proestrus phase also occurred with research on Labrador dogs and German Shepherds conducted by Rao et al (2011) [13] .…”

Section: Discussioncontrasting

confidence: 99%

Vaginal cytology and receptivity to mating behaviour of Kintamani bitch during the proestrus to estrus phase

Nesa¹,

Trilaksana²,

Puja³

2022

Int. J. Vet. Sci. Anim. Husbandry

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The breeding and rearing of Kintamani Bali dogs have evolved from just keeping them as pets to a dog farming industry. Individual variation in female dog fertility must be detected to manage to breed successfully. Vaginal smear cytology is a low-cost and simple method of determining dog's estrus cycle stages. This study aims to determine the vaginal cytology and receptivity to mating behavior in Kintamani Bali bitch during the proestrus to the estrus phase. The investigation started on the first day of proestrus and continued until the first day of receptivity of mating with a male naturally. The results of this study showed that from the beginning of serosanguinous discharge to early estrus, characterized by a gradual change from initially parabasal and intermediate cells (small then larger) and finally to superficial cells. The onset of estrus is characterized by a predominance of superficial cells and their nucleus disappears and is accompanied by a peak of fully cornified cells. In conclusion, was observed a significant difference in cornified cell identification between the proestrus and estrus phases.

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Section: Discussioncontrasting

confidence: 99%

Vaginal cytology and receptivity to mating behaviour of Kintamani bitch during the proestrus to estrus phase

Nesa¹,

Trilaksana²,

Puja³

2022

Int. J. Vet. Sci. Anim. Husbandry

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“…Six healthy dogs were also included in this study for comparison. For confirmatory diagnosis, each bitch was subjected to ultrasonography (Esaotemylab 40, Italy), X-ray, vaginal cytology (Haji et al 2018) and haemato-biochemical analysis. Five milliliters of blood was collected aseptically from each animal with and without EDTA vial to study the haemato-biochemical changes of pyometric and healthy bitches.…”

Section: Methodsmentioning

confidence: 99%

Clinico-haemato-biochemical and Pathological Alteration of Pyometra in Canines

Talukdar

Sarma

Konwar

et al. 2022

IJAR

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Background: Pyometra is a pathological state of the compromised uterus of grown-up to old, diestrum bitches. Pyometra is easy to identify but can be more challenging when there is no vaginal discharge. In the present study, an attempt was made to find out the pathogen involvement and clinico-haemato-biochemical alteration in bitches affected with Pyometra. Methods: Twelve bitches with a history of inappetence, polydipsia, dirty vaginal discharge, occasional vomition irrespective of breeds and age groups were clinically examined and diagnosed as pyometra. The presumptive clinical diagnosis was based on case history, clinical signs, vaginal cytology, abdominal ultrasonography or radiography, gross examination of a pus-filled uterus after the ovariohysterectomy. Result: Vaginal examination revealed brown chocolate malodorous vaginal discharge with discolouration of the perivulval tissue. Abdominal ultrasound showed hyperplasia with echogenic material in the uterine wall along with distended uterine horns containing a large volume of cellular contents. Radiographic imaging revealed cranio-dorsal displacement of the small intestine and a twisted uniform tubular opacity with a distended uterus. The vaginal cytology revealed a large number of degenerated neutrophils, intermediate and parabasal cells. Escherichia coli were the major pathogen isolated from the pyometra uteri of bitches. The surface of the endometrium was columnar ciliated epithelium, with hyperplasia of subepithelial tissue and thick muscularis. The haemato-biochemical evaluation in pyometra infected bitches revealed decreased haemoglobin (Hb), packed cell volume (PCV), total erythrocytic count (TEC) and lymphocyte count indicating the microcytic hypochromic type of anaemia. Leucocytosis with a predominant absolute neutrophilia and lymphocytosis were consistently found in canine pyometra. There was a significantly elevated profile of blood urea nitrogen, creatinine, transaminases and alkaline phosphatase as well as total proteins observed in pyometric female dogs as compared to healthy ones which may have diagnostic and prognostic importance, which also indicated damage of vital organ caused by bacterial endotoxins. The haemato-biochemical indices correlating with imaging techniques, vaginal cytology and histopathological examination can serve as good prognostic markers in bitches with pyometra.

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“…Setelah itu preparat dikeringkan dengan cara diangin-anginkan. Preparat diamati di bawah mikroskop dengan perbesaran 40 x (Haji et al, 2018).…”

Section: Materi Dan Metodeunclassified

Hubungan antara skor hasil bacaan heat detector dengan gambaran sitologi vagina sebagai parameter penentuan estrus pada kambing

Meydilasari¹,

Hernawati²,

Lestari³

et al. 2020

Ovz

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This study aimed to determine the relationship between heat detector reading results and the feature of vaginal swab preparations as a parameter for determining estrus in Peranakan Etawa goats. Five healthy non-pregnant goats weighing 20-30 kg, were synchronized using PGF2α (7.5 mg Dinoprost, Enzaprost) intramuscularly twice with 11 days interval. Vaginal cytology examination began the day after the second PGF2α injection. The heat detector (Draminski®, Dramiński SA, Poland) is an estrus detecting device based on the amount of electric current flowing through the vaginal mucus. Spearman correlation analysis showed a relationship (p <0.05) between heat detector reading and estrus detection based on vaginal cytology. The heat detector readings were very significantly correlated positively (p <0.01) with the number of epithelial cells (coefficient correlation, r= 0.564), but negatively correlated with the number of cornified cells (r = –0.854), and also significantly correlated positively (p <0.05) to the number of leukocytes (r = 0.487). The estrus stages were significantly correlated positively (p <0.05) only to the number of leukocytes (r = 0.918). Likewise, the number of epithelial cells was highly correlated negatively (p <0.01) only to cornified cells (r = –0,643), and the number of cornified cells was significantly correlated negatively (p <0.05) only to the number of leukocytes (r = –0.472). It could be concluded that the features of the proestrus, estrus and metestrus stages based on the goat vaginal cytology was correlated with the reading results of the heat detector.

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The Role of Estrogen and Progesterone Hormone on Vaginal Cytology in Bitch

Cited by 5 publications

References 0 publications

Vaginal cytology and receptivity to mating behaviour of Kintamani bitch during the proestrus to estrus phase

Vaginal cytology and receptivity to mating behaviour of Kintamani bitch during the proestrus to estrus phase

Clinico-haemato-biochemical and Pathological Alteration of Pyometra in Canines

Hubungan antara skor hasil bacaan heat detector dengan gambaran sitologi vagina sebagai parameter penentuan estrus pada kambing

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