The Role of Ethylene in Corolla Unfolding in Ipomoea Nil (Convolvulaceae)

Koning, Ross E.

doi:10.1002/j.1537-2197.1986.tb09690.x

Cited by 13 publications

(8 citation statements)

References 18 publications

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“…The plants were induced to flower under short-day conditions and maintained under long-day conditions to maximize vegetative growth and flower production as previously described ( 13).…”

Section: Plant Culturementioning

confidence: 99%

“…The dishes were incubated in the dark at 32°C, and total filament length was measured daily using a dissecting microscope equipped with a calibrated optical micrometer (13).…”

Section: Stamen and Filament Growth In Vitromentioning

confidence: 99%

“…2) and rapidly convert it to ethylene gas (Fig. 3), which is already known to inhibit the growth of cells in the base of younger corollas (23,24) while promoting cell growth at the rib margins of older corollas (13) for corolla unfolding. Evidence exists that due to the lower levels of ACC and corresponding ethylene in anthers 87 to 21 h before anthesis (Figs.…”

Section: Endogenous Acc Levels and Ethylene Productionmentioning

confidence: 99%

“…Previous work on L nil has examined the roles of environmental stimuli and growth regulators on the growth and development of floral organs (8,(13)(14)(15)(22)(23)(24), corolla unfolding (8,13) and senescence (9,11,16,20).…”

mentioning

confidence: 99%

“…While ethylene levels remained constant in older filaments (15), older corollas produced low levels of ethylene but much higher levels of gibberellin to initiate and sustain rapid cell elongation in the corolla (23,24). ACC and ethylene also promoted corolla unfolding, or flower opening in I. nil (8,13) and floral senescence (9, 1 1, 16, 20). It has been proposed that the anthers of I. nil may contain high levels of ACC or IAA (15,25), both known to stimulate ethylene production, as reported in many other species (5,6,17,26,28).…”

mentioning

confidence: 99%

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Endogenous Levels and Transport of 1-Aminocyclopropane-1-Carboxylic Acid in Stamens of Ipomoea nil (Convolvulaceae)

Kiss¹,

Koning²

1989

Plant Physiol.

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Filament and corolla growth in flowers of lpomoea nil are inhibited by ethylene production. Anthers inhibited filament growth in vitro during younger stages of development even in the presence of the growth promoter gibberellic acid (GA3). To test whether the anthers could be sources of 1-aminocyclopropane-1-carboxylic acid (ACC) endogenous levels of ACC and ethylene production were monitored using gas chromatography. To also test whether the filaments could be transport vectors for ACC the movement of [14CJACC was assessed by scintillation counting from donor agarose blocks, through filament sections, and into receiver agarose blocks. While ACC levels fluctuated in anthers 87 to 21 h before anthesis, anthers contained increased levels of ACC from 15 to 6 hours before anthesis. Ethylene production also fluctuated but peak levels were shifted about 6 hours closer to anthesis than ACC levels within the anthers. Both ACC and ethylene levels in filaments showed fluctuations similar to those in the anthers.[14C]ACC movement became increasingly basipetal during development. Older stages showed greater polar[14C]ACC efflux rates, while all stages showed constant polar influx rates. Low levels of endogenous ACC were transported basipetally from the anther through the filament into agarose blocks at all stages of development. Corresponding levels of endogenous ethylene production remained constant between the varous stages during ACC transport. We have evidence that stamens of 1. nil have a role as source tissues and transport vectors for ACC, to stimulate corolla growth, such as corolla unfolding and senescence.

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“…The plants were induced to flower under short-day conditions and maintained under long-day conditions to maximize vegetative growth and flower production as previously described ( 13).…”

Section: Plant Culturementioning

confidence: 99%

“…The dishes were incubated in the dark at 32°C, and total filament length was measured daily using a dissecting microscope equipped with a calibrated optical micrometer (13).…”

Section: Stamen and Filament Growth In Vitromentioning

confidence: 99%

Section: Endogenous Acc Levels and Ethylene Productionmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Endogenous Levels and Transport of 1-Aminocyclopropane-1-Carboxylic Acid in Stamens of Ipomoea nil (Convolvulaceae)

Kiss¹,

Koning²

1989

Plant Physiol.

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Acid‐induced Filament Elongation in Ipomoea Nil (Convolvulaceae)

Koning

1986

American J of Botany

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It has been known for some time that morning glory filaments elongate in response to increases in concentration of gibberellins (Murakami, 1973) and decreases in ethylene production (Koning and Raab, in press), but many other aspects of their growth have remained unstudied. In the present work, the possible role of gibberellin‐stimulated proton efflux in filament growth was examined. Although applied gibberellins stimulated extensive filament growth in vitro and the pH of the incubating medium became acidified during growth, gibberellin also induced growth in media buffered at alkaline pH values. Acidic buffers alone elicited only a very small amount of growth. Fusicoccin, a potent stimulator of proton efflux, initially stimulated the rate of filament growth but elicited only a small increment of growth. In fact, continued presence of fusicoccin poisoned sustained gibberellin‐induced growth. Vanadate ions, believed to inhibit proton efflux, had little effect upon gibberellin‐induced growth except at extremely high concentrations. Based upon these results, it appears that the acid‐induced component of growth stimulation by gibberellin is relatively minor in Ipomoea filaments. These results are quite different from those reported for filament elongation in Gaillardia (Koning, 1983a).

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Parameters of Filament Elongation in Ipomoea Nil (Convolvulaceae)

Koning

Raab

1987

American J of Botany

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It has been thought for some time that morning glory filaments elongate in response to changes in concentrations of gibberellins (Murakami, 1973), but many other aspects of their growth have remained unstudied. In the present work, the interacting roles of gibberellin and ethylene in filament growth were examined. Filaments elongated ten‐fold by epidermal cell elongation accompanied by ten‐fold increases in fresh and dry weight. Applied gibberellins could stimulate filament growth in vitro, but gibberellin biosynthesis inhibitors had no effect. The putative gibberellin action inhibitor, ancymidol, reduced growth but the inhibition could be removed by blocking ethylene biosynthesis. Stimulators of the ethylene biosynthesis pathway and applied ethylene precursor (ACC) strongly inhibited filament elongation; ethylene biosynthesis inhibitors elicited as much growth as applied gibberellin. The filaments produced little ethylene at the time of the onset of growth. While the filaments produced ethylene rapidly before and after growth initiation, the closed flower bud had a relatively constant level of ethylene. It seems likely that in situ production of ethylene negatively influences filament growth.

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The Role of Ethylene in Corolla Unfolding in Ipomoea Nil (Convolvulaceae)

Cited by 13 publications

References 18 publications

Endogenous Levels and Transport of 1-Aminocyclopropane-1-Carboxylic Acid in Stamens of Ipomoea nil (Convolvulaceae)

Endogenous Levels and Transport of 1-Aminocyclopropane-1-Carboxylic Acid in Stamens of Ipomoea nil (Convolvulaceae)

Acid‐induced Filament Elongation in Ipomoea Nil (Convolvulaceae)

Parameters of Filament Elongation in Ipomoea Nil (Convolvulaceae)

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