2021
DOI: 10.1002/bit.27760
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The role of extracellular DNA in the formation, architecture, stability, and treatment of bacterial biofilms

Abstract: Advances in biotechnology to treat and cure human disease have markedly improved human health and the development of modern societies. However, substantial challenges remain to overcome innate biological factors that thwart the activity and efficacy of pharmaceutical therapeutics. Until recently, the importance of extracellular DNA (eDNA) in biofilms was overlooked. New data reveal its extensive role in biofilm formation, adhesion, and structural integrity. Different approaches to target eDNA as anti-biofilm t… Show more

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Cited by 93 publications
(58 citation statements)
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“…This finding suggests that the mode of action of intrapleural DNase therapy may be to inhibit early biofilm formation in pleural empyema. eDNA is known to be important for biofilm stability, and DNase treatment can remove eDNA from biofilms, particularly during bacterial attachment and the initial stages of biofilm formation (Panlilio and Rice, 2021). Cleavage of eDNA by DNase can promote the penetration of antibiotic molecules and decrease biofilm biomass (Liu et al, 2020).…”
Section: Discussionmentioning
confidence: 99%
“…This finding suggests that the mode of action of intrapleural DNase therapy may be to inhibit early biofilm formation in pleural empyema. eDNA is known to be important for biofilm stability, and DNase treatment can remove eDNA from biofilms, particularly during bacterial attachment and the initial stages of biofilm formation (Panlilio and Rice, 2021). Cleavage of eDNA by DNase can promote the penetration of antibiotic molecules and decrease biofilm biomass (Liu et al, 2020).…”
Section: Discussionmentioning
confidence: 99%
“…It could explain the strong contrasting action towards the biofilm growth, since both EPS and proteins play an essential role in biofilm expansion other then as protective agents (Karygianni et al ., 2020). Also, extracellular DNA aids in bacterial attachment and aggregation, but it is critical especially in the early stages of biofilm formation (Panlilio and Rice, 2021).…”
Section: Discussionmentioning
confidence: 99%
“…The formation of C. difficile biofilms in vitro is typically studied in monoculture, but in vivo, interactions with other bacterial species, either pathogens or commensal members of the gut microbiome, may play an important role (14,15), either cooperatively or competitively. Bacterial biofilms are composed not only of bacterial cells, but an extensive extracellular matrix composed of polysaccharides, extracellular DNA and polypeptides (16)(17)(18)(19), either as monomers (20) or as surface assemblies (21,22). Previously, our group and others have reported a defect in in vitro biofilm formation for mutations in the Type IV pilus (T4P) system in C. difficile (7)(8)(9).…”
Section: Introductionmentioning
confidence: 99%
“…Bacterial biofilms are composed not only of bacterial cells but an extensive extracellular matrix composed of polysaccharides, extracellular DNA, and polypeptides ( 16 , 17 , 18 , 19 ), either as monomers ( 20 ) or as surface assemblies ( 21 , 22 ). Previously, our group and others have reported a defect in in vitro biofilm formation for mutations in the type IV pilus (T4P) system in C. difficile ( 7 , 8 , 9 ).…”
mentioning
confidence: 99%