The Role of Fast Cell Cycle Analysis in Pediatric Brain Tumors

Alexiou, George Α.; Vartholomatos, George; Stefanaki, Kalliopi; Lykoudis, Efstathios G.; Patereli, Amalia; Tseka, Georgia; Tzoufi, Meropi; Sfakianos, George; Prodromou, Neofytos

doi:10.1159/000439029

Cited by 23 publications

(13 citation statements)

References 17 publications

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“…PK-CA577-K920; PromoCell GmbH, Heidelberg, Germany). The number of myocardial cells in S, G 2 and M phases were analyzed according to a previously published study ( 27 ).…”

Section: Methodsmentioning

confidence: 99%

Isoflurane reduces pain and inhibits apoptosis of myocardial cells through the phosphoinositide 3-kinase/protein kinaseï¿½B signaling pathway in mice during cardiac surgery

Lin

Yang

2018

Mol Med Report

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Heart bypass surgery is the most common treatment for myocardial ischemia. Clinical investigations have revealed that isoflurane anesthesia is efficient to alleviate pain during cardiac surgery, including heart bypass surgery. Previous studies have revealed the protective effects of isoflurane on myocardial cells of patients with myocardial ischemia during the perioperative period. The present study aimed to investigate the mechanism underlying the protective effects of isoflurane on myocardial cells in mice with myocardial ischemia. ELISA, flow cytometry, immunofluorescence and western blotting were used to analyze the effects of isoflurane anesthesia on myocardial cells. Briefly, myocardial cell apoptosis and viability, pain, phosphoinositide 3-kinase/protein kinase B (PI3K/AKT) signaling pathway expression and the pharmacodynamics of isoflurane were studied in mice treated with isoflurane for heart bypass surgery. The results demonstrated that isoflurane anesthesia efficiently attenuated pain in mice during surgery. Viability and apoptosis of myocardial cells was also improved by isoflurane in vitro and in vivo. The PI3K/AKT pathway was upregulated in myocardial cells on day 3 post-operation. Mechanistically, isoflurane promoted PI3K/AKT activation, upregulated B-cell lymphoma 2 (Bcl-2)-associated X protein and Bcl-2 expression levels, and reduced the expression levels of caspase-3 and caspase-8 in myocardial cells. In conclusion, the findings indicated that isoflurane is beneficial for pain attenuation and inhibits apoptosis of myocardial cells via the PI3K/AKT signaling pathway in mice during cardiac surgery.

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“…PK-CA577-K920; PromoCell GmbH, Heidelberg, Germany). The number of myocardial cells in S, G 2 and M phases were analyzed according to a previously published study ( 27 ).…”

Section: Methodsmentioning

confidence: 99%

Isoflurane reduces pain and inhibits apoptosis of myocardial cells through the phosphoinositide 3-kinase/protein kinaseï¿½B signaling pathway in mice during cardiac surgery

Lin

Yang

2018

Mol Med Report

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“…indeed, it is known that hypertension in the perioperative period of a craniotomy is associated with acute hemorrhagic complications. [1][2][3] By modifying a previously published protocol reported by our group, we are able to provide, within 6 minutes, rapid cell cycle analysis. Unfortunately, these data could not be captured in the current study because many of these patients did not have continual blood pressure monitoring.…”

Section: Responsementioning

confidence: 99%

“…Unfortunately, these data could not be captured in the current study because many of these patients did not have continual blood pressure monitoring. 1, 3 Shioyama et al also demonstrated that flow cytometry may be feasible to determine the presence of glioma in a surgical biopsy sample. .…”

Section: Responsementioning

confidence: 99%

Letter to the Editor: Innovations in neurosurgery

Farber¹,

Thomas²,

Pace³

et al. 2016

JNS

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“…Currently, we are investigating the value of cell cycle analysis for rapid intraoperative characterization of brain tumors and for the identification of tumor margins. [1][2][3] By modifying a previously published protocol reported by our group, we are able to provide, within 6 minutes, rapid cell cycle analysis. 1 Based on G 0 / G 1 , S-phase, G 2 /M phase fraction, and ploidy status, highgrade tumors could be differentiated from low-grade tumors and neoplastic from normal brain tissue with high sensitivity and specificity, both for adult and pediatric brain tumors.…”

Section: Judith Marcoux MD Msc Frcsc Rajeet Singh Saluja Md Frcscmentioning

confidence: 99%

“…1 Based on G 0 / G 1 , S-phase, G 2 /M phase fraction, and ploidy status, highgrade tumors could be differentiated from low-grade tumors and neoplastic from normal brain tissue with high sensitivity and specificity, both for adult and pediatric brain tumors. 1,3 Shioyama et al also demonstrated that flow cytometry may be feasible to determine the presence of glioma in a surgical biopsy sample. 5 Furthermore, we proposed the development of a device that would combine the Cavitron ultrasonic surgical aspirator with a real-time flow cytometer.…”

Section: Judith Marcoux MD Msc Frcsc Rajeet Singh Saluja Md Frcscmentioning

confidence: 99%

Letter to the Editor: Nonsurgical acute traumatic subdural hematoma

Atalay¹,

Ak²

2016

JNS

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authors report on 27 patients with recurrent Grade 2 meningioma treated with radiosurgery alone. Patients were treated with a mean of 15.2 Gy. The actuarial local control rates were 75%, 52%, and 40% at 12, 24, and 36 months, respectively. The results of this study do not significantly differ from those of other series (recently reviewed in rogers et al., 2015 2), with the exception that increased treatment doses appear to yield somewhat higher control rates than those seen in this study, as noted by the authors. The authors conclude that radiosurgery is safe and effective for local control of recurrent atypical meningiomas. While we do not dispute the lessened short-term morbidity of radiosurgery compared with surgery, particularly at the doses used in this study, we would suggest that a 40% local control rate (i.e., a 60% failure rate) at 3 years does not indicate efficacy , as suggested by the authors. For reference, we have overlaid the authors' progression-free survival Kaplan-Meier plot on the recurrence-free survival plot of Grade 2 meningiomas, which shows the course of these tumors with no treatment (Fig. 1). We acknowledge that there are limitations in this comparison, but to us, it calls into question the effect of radiosurgery on the tumor in that it does not seem to alter the natural history of the disease. Based on this study and others, we would argue that radiosurgery alone is not sufficient therapy for Grade 2 meningiomas and should be reserved for the treatment of recurrence in patients who cannot undergo a second resection.

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The Role of Fast Cell Cycle Analysis in Pediatric Brain Tumors

Cited by 23 publications

References 17 publications

Isoflurane reduces pain and inhibits apoptosis of myocardial cells through the phosphoinositide 3-kinase/protein kinaseï¿½B signaling pathway in mice during cardiac surgery

Isoflurane reduces pain and inhibits apoptosis of myocardial cells through the phosphoinositide 3-kinase/protein kinaseï¿½B signaling pathway in mice during cardiac surgery

Letter to the Editor: Innovations in neurosurgery

Letter to the Editor: Nonsurgical acute traumatic subdural hematoma

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