The Role of Feeding Characteristics in Shaping Gut Microbiota Composition and Function of Ensifera (Orthoptera)

Xy, Zheng; Zhu, Qidi; Meng, Qingcai; Zhou, Zhijun; Liu, Chunmao; Wang, Liyuan; Shi, Fu-Ming

doi:10.3390/insects13080719

Cited by 6 publications

(3 citation statements)

References 68 publications

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“…Previous studies have shown that feeding characteristics are the primary factor affecting the structure of the gut bacterial communities of insects [ 36 , 37 ]. The horizontal transmission of food has a significant effect on the bacterial structure of the insect gut, and some bacteria are not able to colonize the body in the absence of external bacterial supplementation, causing changes in the dominant species or core bacterial groups at different life stages [ 38 ].…”

Section: Discussionmentioning

confidence: 99%

The species and abundance of gut bacteria both positively impact Phortica okadai behavior

Li,

Wang,

Wang

et al. 2024

Parasites Vectors

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Background Gut bacteria, which serve as essential modulators, exert a significant impact on insect physiology and behavior and have substantial application potential in pest management. The dynamics of gut bacteria and their impact on Phortica okadai behavior remain unclear. Methods In this study, the dynamics of gut bacteria at different developmental stages in P. okadai were analyzed using 16S ribosomal RNA (rRNA) gene sequencing, and the species and abundance of gut bacteria that affect host behavior were examined via behavioral experiments. Results A total of 19 phyla, 29 classes, 74 orders, 101 species, and 169 genera were identified. The results of the behavioral experiments indicated that the species Lactiplantibacillus argentoratensis, Acetobacter tropicalis, Leuconostoc citreum, and Levilactobacillus brevis effectively influenced the feeding preference of P. okadai, and the single-bacterium-seeded P. okadai exhibited feeding preferences distinct from those of the germ-free (GF) and wild-type P. okadai. Conclusions The species and relative abundance of gut bacteria together positively impact P. okadai behavior. Lactiplantibacillus argentoratensis, as the most attractive bacteria to P. okadai, presents opportunities for novel pest control strategies targeting this vector and agricultural pest. Graphical Abstract

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Section: Discussionmentioning

confidence: 99%

The species and abundance of gut bacteria both positively impact Phortica okadai behavior

Li,

Wang,

Wang

et al. 2024

Parasites Vectors

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“…Insect gut microbial communities co-evolve with their hosts, enabling insects to adapt to different diets and overcome the limitations of obtaining nutrients from food and countering defensive chemicals ( 3 ). Insect guts commonly harbor bacterial families such as Enterobacteriaceae, Pseudomonadaceae, and Lactobacillaceae, which play vital roles in digestion, immunity, detoxification, and reproduction processes ( 4 – 6 ). For instance, in Drosophila , Enterobacteriaceae aids in digestion and absorption by degrading carbohydrates in the intestinal tract ( 7 ).…”

Section: Introductionmentioning

confidence: 99%

Gut microorganisms of Locusta migratoria in various life stages and its possible influence on cellulose digestibility

Li,

Liang

et al. 2024

mSystems

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Locusta migratoria is an important phytophagous pest, and its gut microbial communities play an important role in cellulose degradation. In this study, the gut microbial and cellulose digestibility dynamics of Locusta migratoria were jointly analyzed using high-throughput sequencing and anthrone colorimetry. The results showed that the gut microbial diversity and cellulose digestibility across life stages were dynamically changing. The species richness of gut bacteria was significantly higher in eggs than in larvae and imago, the species richness and cellulose digestibility of gut bacteria were significantly higher in early larvae (first and second instars) than in late larvae (third to fifth instars), and the diversity of gut bacteria and cellulose digestibility were significantly higher in imago than in late larvae. There is a correlation between the dynamics of gut bacterial communities and cellulose digestibility. Enterobacter , Lactococcus, and Pseudomonas are the most abundant genera throughout all life stages. Six strains of highly efficient cellulolytic bacteria were screened, which were dominant gut bacteria. Carboxymethyl cellulase activity (CMCA) and filter paper activity (FPA) experiments revealed that Pseudomonas had the highest cellulase enzyme activity. This study provides a new way for the screening of cellulolytic bacteria and lays the foundation for developing insects with significant biomass into cellulose-degrading bioreactors. IMPORTANCE Cellulose is the most abundant and cheapest renewable resource in nature, but its degradation is difficult, so finding efficient cellulose degradation methods is an urgent challenge. Locusta migratoria is a large group of agricultural pests, and the large number of microorganisms that inhabit their intestinal tracts play an important role in cellulose degradation. We analyzed the dynamics of Locusta migratoria gut microbial communities and cellulose digestibility using a combination of high-throughput sequencing technology and anthrone colorimetry. The results revealed that the gut microbial diversity and cellulose digestibility were dynamically changed at different life stages. In addition, we explored the intestinal bacterial community of Locusta migratoria across life stages and its correlation with cellulose digestibility. The dominant bacterial genera at different life stages of Locusta migratoria were uncovered and their carboxymethyl cellulase activity (CMCA) and filter paper activity (FPA) were determined. This study provides a new avenue for screening cellulolytic bacteria and lays the foundation for developing insects with significant biomass into cellulose-degrading bioreactors.

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“…We previously developed Autometa, an automated binning pipeline that is able to effectively recover genomes from highly convoluted environmental and non-model host-associated microbial communities (3). This tool has seen widespread use in environments ranging from marine, freshwater and terrestrial samples, including corals (4), red algae (5), kinetoplastids (6), deep sea geothermal vents (7)(8)(9), sponges (10)(11)(12), coastal sediments (13), stromatolites (14), seaweeds (15), shipworms (16), plateau lakes (17,18), hot springs (19,20), contaminated rivers (21), beetles (22)(23)(24)(25), Kickxellomycotina fungi (26), Ensifera insects (27), fermented agave (28,29), a marsh orchid rhizobiome (30), domesticated cattle (31,32), mice (33) and human gut (34), periodontal (35) as well as urinary tract (36) microbiomes. As a consequence of Autometa's widespread use, both non-technical and technical researchers alike have communicated their frustrations regarding the ease of installation as well as the efficiency and robustness throughout the various stages of the Autometa workflow.…”

Section: Introductionmentioning

confidence: 99%

Autometa 2: A versatile tool for recovering genomes from highly-complex metagenomic communities

Rees,

Uppal,

Clark

et al. 2023

Preprint

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In 2019, we developed Autometa, an automated binning pipeline that is able to effectively recover metagenome-assembled genomes from complex environmental and non-model host-associated microbial communities. Autometa has gained widespread use in a variety of environments and has been applied in multiple research projects. However, the genome-binning workflow was at times overly complex and computationally demanding. As a consequence of Autometa's diverse application, non-technical and technical researchers alike have noted its burdensome installation and inefficient as well as error-prone processes. Moreover its taxon-binning and genome-binning behaviors have remained obscure. For these reasons we set out to improve its accessibility, efficiency and efficacy to further enable the research community during their exploration of Earth's environments. The highly augmented Autometa 2 release, which we present here, has vastly simplified installation, a graphical user interface and a refactored workflow for transparency and reproducibility. Furthermore, we conducted a parameter sweep on standardized community datasets to show that it is possible for Autometa to achieve better performance than any other binning pipeline, as judged by Adjusted Rand Index. Improvements in Autometa 2 enhance its accessibility for non-bioinformatic oriented researchers, scalability for large-scale and highly-complex samples and interpretation of recovered microbial communities.

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The Role of Feeding Characteristics in Shaping Gut Microbiota Composition and Function of Ensifera (Orthoptera)

Cited by 6 publications

References 68 publications

The species and abundance of gut bacteria both positively impact Phortica okadai behavior

The species and abundance of gut bacteria both positively impact Phortica okadai behavior

Gut microorganisms of Locusta migratoria in various life stages and its possible influence on cellulose digestibility

Autometa 2: A versatile tool for recovering genomes from highly-complex metagenomic communities

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