The role of flavonoid antioxidant, morin in improving procarbazine-induced oxidative stress on testicular function in rat

Abstract

Background:Procarbazine (PCZ) is an effective chemotherapeutic drug used in the treatment of lymphoma; however, oxidative stress–mediated testicular toxicity is a major side effect. Recently, therapeutic intervention using flavonoids against oxidative stress–related pathologies is gaining more attention. Morin (MOR) is a natural flavonoid with proven antioxidant activity. This study was designed therefore to evaluate the potential role of MOR in ameliorating PCZ-induced testicular oxidative stress and altered sperm quality in rat model.Methods:A total of 24 male Wistar rats (170–180 g) were randomly assigned into 4 treatment groups: I, control; II, PCZ (2 mg/kg b.w.); III, PCZ (2 mg/kg b.w.) + MOR (100 mg/kg b.w.) simultaneously administered and IV, MOR (100 mg/kg b.w.), and all treatments lasted 14 days.Results:PCZ treatment displayed significant reduction in sperm number, sperm motility, percentage normal sperm cells, and daily sperm production rate. Meanwhile the activities of testicular enzymes: gamma-glutamyl transferase, acid phosphatase, and lactate dehydrogenase were significantly altered in the PCZ group compared to control. Furthermore, PCZ caused a significant reduction in levels of glutathione and ascorbic acid as well as activities superoxide dismutase, catalase, glutathione peroxidase, and glutathione S-transferase in the testes of PCZ-treated rats. A significant increase in testicular malondialdehyde level was also observed in the PCZ group. MOR treatment, however, significantly restored the altered sperm parameters and biochemical markers in the testis.Conclusions:Our data suggest that MOR administration protected against PCZ-induced testicular and spermatotoxicity in rat, by improving testicular antioxidant system.

Preservation of antioxidant defense system by Morin in bicalutamide-induced rat testicular toxicity

“…The increase in abnormal sperm coupled with decrease in motility, viability and total sperm cell count is consistent with an earlier report on BCT (Adeniyi et al, 2009;Abdulrahman and Mustafa, 2019). A significant improvement was observed when MOR was administered along with BCT which is in agreement with previous reports on the protective effects of MOR on testicular function (Olayinka et al, 2019).…”

“…represents the dosage for the treatment of a locally advanced prostate cancer, 100 mg/kg/d MOR p.o. was selected on the basis of its effectiveness in earlier studies (Kolvenbag and Nash, 1999;Kuzu et al, 2019;Olayinka et al, 2019).…”

“…In addition, it possesses a number of other effects including the immunomodulation and anticancer activity (Caselli et al, 2016;Sinha et al, 2016). Our earlier studies showed that MOR protected against sperm alteration and testicular oxidative stress induced by procarbazine (an alkylating agent) in rat (Olayinka et al, 2019). Previous studies have shown that co-administration of anticancer drugs and antioxidants compounds protected against chemotherapy-induced toxicities and improve the overall therapeutic outcome (Singh et al, 2018).…”

Effect of Hesperetin Treatment on Blood Glucose Level, Spermatozoa Quality, and Spermatozoa Quantity in Alloxan-Induced Diabetic Mice

“…This increases fertility and decreases the percentage of abnormal spermatozoa. It produces a positive feedback loop that also leads to increased testosterone levels (Olayinka et al, 2019). The normal shape of the spermatozoa will directly affect the spermatozoa motility and integrity (Jakubik-Uljasz et al, 2020).…”

Morin-5′-Sulfonic Acid Sodium Salt (NaMSA) Attenuates Cyclophosphamide-Induced Histological Changes in Genitourinary Tract in Rats—Short Report

“…Testicular toxicity is also an adverse effect of some other anticancer agents, e.g., procarbazine. Olaynka et al [28] found that morin significantly restored the procarbazine-induced abnormal sperm parameters (count, motility, percentage of normal sperm). Some studies claim that morin protects testicular tissue from morphological injuries and pathology of sperm count and viability induced by titanium dioxide nanoparticles [27,29].…”