The Role of Formylpeptide Receptors, C5a Receptors, and Cytosolic-Free Calcium in Neutrophil Priming

Zimmerli, Werner; Reber, Anne-Marie; Dahinden, Clemens A.

doi:10.1093/infdis/161.2.242

Cited by 39 publications

(18 citation statements)

References 34 publications

(29 reference statements)

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“…While it is tempting to propose a cause-andeffect mechanism, studies with other priming agents, including ATP, platelet activating factor, tumor necrosis factor-␣, interleukin (IL)-8, LPS, and granulocyte macrophage-colony stimulating factor, 29 have demonstrated that an increase in fMLP receptors is not necessarily required for an increased oxidative burst response to fMLP activation. Zimmerli et al 30 using neutrophils primed with LPS found neutrophil oxidative burst was increased in response to C5a in the absence of up-regulation of C5a receptors. These results argue against there being a direct association between an increase in receptor number and the responsiveness of primed cells.…”

Section: Discussionmentioning

confidence: 99%

Neutrophil Priming by Cigarette Smoke Condensate and a Tobacco Anti-Idiotypic Antibody

Koethe

Kuhnmuench

Becker

2000

The American Journal of Pathology

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A polyphenol-rich reagent, referred to as CSC, was isolated from cigarette smoke condensate and shown to prime purified human neutrophils. A mouse monoclonal anti-idiotypic antibody directed against the polyphenol-reactive determinants on a rabbit polyclonal anti-tobacco glycoprotein antibody was generated and shown to also prime neutrophils. After priming by CSC or tobacco anti-idiotypic antibody, there was a 2.5-fold to threefold increase in CD11b/18 expression and doubling of the number of formylmethionyl-leucyl-phenylalanine receptors on the cells. The primed cells showed a twofold increase, compared with unprimed cells, in production of superoxide and release of neutrophil elastase after stimulation with formyl-methionyl-leucyl-phenylalanine. Neutrophils in peripheral blood of cigarette smokers have been shown to be primed and more responsive to activating agents. The priming effects attributed to whole cigarette smoke have been demonstrated in these studies using purified neutrophils and CSC or tobacco anti-idiotypic antibody. These studies are a first step in testing the hypothesis that the inflammatory process contributing to progression of chronic obstructive pulmonary disease in exsmokers may be driven , in part , by tobacco antiidiotypic antibodies. This hypothesis is novel and carries with it the implication of a heretofore unrecognized autoimmune component in the disease process manifested through production of anti-idiotypic antibodies with tobacco-like activity. (Am J Pathol 2000, 157:1735-1743)

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Section: Discussionmentioning

confidence: 99%

Neutrophil Priming by Cigarette Smoke Condensate and a Tobacco Anti-Idiotypic Antibody

Koethe

Kuhnmuench

Becker

2000

The American Journal of Pathology

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“…Previously, a number of groups have shown that pre-exposure of PMNs to a priming agent resulted in a significantly enhanced respiratory burst when the cell was subsequently activated [50][51][52][53][55][56][57]. In addition, priming has also been shown to cause a number of other cellular responses in PMNs, including changes in [Ca 2ϩ ] i [56,63], changes in expression of adhesion molecules and NADPH oxidase proteins [60][61][62], changes in expression of receptors [63,68], membrane depolarization [63], and increased polymerization of actin [52,69,70]. In this report, we show that treatment of PMNs with peroxynitrite does not directly activate PMNs but induces many of the priming events described above for other known priming agents.…”

Section: Discussionmentioning

confidence: 99%

“…Priming by a number of agents has also been shown to cause [56,63], and changes in [Ca 2ϩ ] i appear to be among the earliest events in signaling PMNs for subsequent physiological responses [64]. Therefore, we investigated whether peroxynitrite treatment of PMNs could also elicit a rise in [Ca 2ϩ ] i .…”

Section: Priming Of Pmns By Peroxynitritementioning

confidence: 99%

Priming of human neutrophils by peroxynitrite: potential role in enhancement of the local inflammatory response

Rohn

Nelson

Sipes

et al. 1999

Journal of Leukocyte Biology

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“…An ideal marker should be specific for the compartment in question, unaffected by stimulation, and the assay used for detection should be easy to perform and reproducible. Various assays for the detection of plasma membrane-specific proteins, such as the receptors for PAF [80] and C5a [81] have been used, but due to methodological disadvantages, they have been abandoned.…”

Section: Alternative Markers For the Plasma Membranementioning

confidence: 99%

Localization of human neutrophil interleukin-8 (CXCL-8) to organelle(s) distinct from the classical granules and secretory vesicles

Pellmé

Mörgelin

Tapper

et al. 2005

Journal of Leukocyte Biology

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Mature human neutrophils contain small amounts of interleukin‐8 [CXC chemokine ligand 8 (CXCL‐8)], which upon proinflammatory activation, increases significantly. It has been suggested that the CXCL‐8 content of resting human neutrophils is stored in the secretory vesicles. Here, we have used a fractionation technique, which allows isolation of these vesicles, and we find that CXCL‐8 neither colocalizes with the secretory vesicles nor with markers of any of the classical neutrophil granules. To increase resolution in the system, we induced CXCL‐8 production by lipopolysaccharide. After 8 h of stimulation, CXCL‐8 was visualized within the cell using immunoelectron microscopy. The images revealed CXCL‐8‐containing stuctures resembling neutrophil granules, and these were distinct from all known neutrophil organelles, as shown by double immunostaining. Further, the CXCL‐8 organelle was present in nonstimulated neutrophil cytoplasts, entities lacking all other known granules and secretory vesicles. Upon fractionation of the cytoplasts, CXCL‐8 was found to partly cofractionate with calnexin, a marker for endoplasmic reticulum (ER). Thus, part of CXCL‐8 may be localized to the ER or ER‐like structures in the neutrophil.

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The Role of Formylpeptide Receptors, C5a Receptors, and Cytosolic-Free Calcium in Neutrophil Priming

Cited by 39 publications

References 34 publications

Neutrophil Priming by Cigarette Smoke Condensate and a Tobacco Anti-Idiotypic Antibody

Neutrophil Priming by Cigarette Smoke Condensate and a Tobacco Anti-Idiotypic Antibody

Priming of human neutrophils by peroxynitrite: potential role in enhancement of the local inflammatory response

Localization of human neutrophil interleukin-8 (CXCL-8) to organelle(s) distinct from the classical granules and secretory vesicles

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