The role of Galectin-1 in the interaction between chondrocytes and a lactose-modified chitosan

Marcon, Patrizia; Marsich, Eleonora; Vetere, Amedeo; Mozetic, Pamela; Campa, Cristiana; Donati, Ivan; Vittur, Franco; Gamini, Amelia; Paoletti, Sergio

doi:10.1016/j.biomaterials.2005.01.044

Cited by 51 publications

(60 citation statements)

References 50 publications

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“…The observed biphasic effect of galectin-1 during cell-cell and cell-extracellular matrix interactions is in agreement with several earlier results [Hughes, 2001;Camby et al, 2006]. Recently, Marcon et al [2005] described that galectin-1 stimulates chondrocyte growth on a lactosemodified chitosan scaffold and inhibits incorporation of CSA into the extracellular matrix of vascular smooth muscle cells [Moiseeva et al, 2003]. …”

Section: Discussionsupporting

confidence: 80%

Chondrogenic Potential of Mesenchymal Stem Cells from Patients with Rheumatoid Arthritis and Osteoarthritis: Measurements in a Microculture System

Dudics

Kunstár

Kovács

et al. 2008

Cells Tissues Organs

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Background: Mesenchymal stem cells (MSCs) have the potential to differentiate into distinct mesenchymal tissues; including cartilage and bone, they can be an attractive cell source for cartilage tissue engineering approaches. Our objective here was to compare the in vitro chondrogenic potential of MSCs isolated from patients with rheumatoid arthritis (RA) and osteoarthritis (OA) with cells from normal donors. Methods: Marrow samples were removed during bone surgery and adherent cell cultures were established. The cells were then passed into a newly developed microaggregate culture system in a medium containing transforming growth factor β3, insulin, dexamethasone and/or demineralized bone matrix. In vitro chondrogenic activity was measured as metabolic sulfate incorporation and type II collagen expression in pellet cultures. Results: Culture-expanded MSCs from RA and OA patients did not differ significantly from the normal population with respect to their chondrogenic potential in vitro. Capability of total protein and proteoglycan synthesis as well as collagen II mRNA expression by cell aggregates was similar for all cell preparations in the presence of the appropriate growth and differentiation factors. Chondroprotective drugs such as chondroitin sulfate and glucosamine enhanced, whereas chloroquine inhibited chondrogenesis in normal donor-derived or patient-derived MSC cultures. Galectin-1, a β-galactoside-binding protein with marked anti-inflammatory activity, stimulated the chondrogenic differentiation of mesenchymal cells in low (<2 μg/ml) concentration. Discussion: These findings show that MSCs from RA and OA patients possess similar chondrogenic potential as MSCs isolated from healthy donors, therefore these cells may serve as a potential new prospect in cartilage replacement therapy.

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Section: Discussionsupporting

confidence: 80%

Chondrogenic Potential of Mesenchymal Stem Cells from Patients with Rheumatoid Arthritis and Osteoarthritis: Measurements in a Microculture System

Dudics

Kunstár

Kovács

et al. 2008

Cells Tissues Organs

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“…The interaction of chondrocytes with ECM proteins induces multiple cellular responses mediated by specific membrane and intracellular glycosylated receptors. Recently, Galectin-1 was suggested to play an important role in the biology of chondrocytes and cartilage regeneration (Marcon et al, 2005). Galectin-1 mediates the interaction between chondrocytes and a semi-synthetic glycopolymer that promotes aggregation of chondrocytes and production of type-II collagen and glycosaminoglycans (Marcon et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Galectin-1 in cartilage: Expression, influence on chondrocyte growth and interaction with ECM components

et al. 2008

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“…Of relevance, the presence of several galectins has been evidenced by detection of specific autoantibodies and their clinical implications in autoimmune diseases (for recent group detections, please see Sarter et al 2013; for general review on galectins in rheumatoid arthritis, please see li et al 2013). Testing pig chondrocytes, the presence of gal-1 and roles in adhesion, extracellular matrix production and shifting metabolism from anabolic to catabolic processes were reported (Marcon et al 2005;Marsich et al 2008). Moreover, attested by in vitro studies, galectins form a network of effectors, which can cooperate or even lead to antagonism by competitive inhibition (Kopitz et al 2001;Sanchez-ruderisch et al 2010;Tsai et al 2011).…”

mentioning

confidence: 96%

Human osteoarthritic knee cartilage: fingerprinting of adhesion/growth-regulatory galectins in vitro and in situ indicates differential upregulation in severe degeneration

Toegel

Bieder

André

et al. 2014

Histochem Cell Biol

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The apparent connection of galectin-3 to chondrocyte survival and osteoarthritis-like cartilage modifications in animal models provided incentive for the mapping of seven members of this family of adhesion/growth-regulatory proteins in human cartilage specimens. Starting with work in vitro, RT-qPCR analyses and immunocytochemistry revealed gene transcription and protein presence in cultured OA chondrocytes, especially for galectin-1, galectin-3 and galectin-8. Immunohistochemistry in clinical specimens with mild and severe cartilage degeneration detected galectins in chondrocytes-with upregulation, especially of galectin-1 in areas of severe degeneration-accompanied by α2,6-sialylation in the pericellular matrix. Given the possibility for additive/antagonistic activities between galectins, these results direct further research toward examining cellular effects of (1) these proteins (alone or in combination) on chondrocytes and (2) remodeling of the chondrocyte glycophenotype.

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The role of Galectin-1 in the interaction between chondrocytes and a lactose-modified chitosan

Cited by 51 publications

References 50 publications

Chondrogenic Potential of Mesenchymal Stem Cells from Patients with Rheumatoid Arthritis and Osteoarthritis: Measurements in a Microculture System

Chondrogenic Potential of Mesenchymal Stem Cells from Patients with Rheumatoid Arthritis and Osteoarthritis: Measurements in a Microculture System

Galectin-1 in cartilage: Expression, influence on chondrocyte growth and interaction with ECM components

Human osteoarthritic knee cartilage: fingerprinting of adhesion/growth-regulatory galectins in vitro and in situ indicates differential upregulation in severe degeneration

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