The Role of Glucose-6-phosphate Dehydrogenase in the Wine Yeast Hanseniaspora uvarum

Heinisch, Jürgen J.; Murra, Andrea; Fernández Murillo, Lucía; Schmitz, Hans-Peter

doi:10.3390/ijms25042395

Cited by 3 publications

(1 citation statement)

References 63 publications

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“…This enzyme catalyzes the interconversion of glucose-6-phosphate and fructose-6-phosphate, linking glycolysis to the pentose phosphate pathway. By modulating the activity or expression of pgi, researchers can manipulate carbon flux towards the pentose phosphate pathway, increasing the availability of precursor metabolites for flavonoid biosynthesis [85,86]. Generally, through genomic editing techniques, such as CRISPR-Cas9mediated strategies, researchers aim to modulate NADPH availability, thereby influencing the flux of carbon towards flavonoid precursors.…”

Section: Future Perspectives and Conclusionmentioning

confidence: 99%

Metabolic Engineering of Corynebacterium glutamicum for the Production of Flavonoids and Stilbenoids

Chu,

Tran,

Pham

et al. 2024

Molecules

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Flavonoids and stilbenoids, crucial secondary metabolites abundant in plants and fungi, display diverse biological and pharmaceutical activities, including potent antioxidant, anti-inflammatory, and antimicrobial effects. However, conventional production methods, such as chemical synthesis and plant extraction, face challenges in sustainability and yield. Hence, there is a notable shift towards biological production using microorganisms like Escherichia coli and yeast. Yet, the drawbacks of using E. coli and yeast as hosts for these compounds persist. For instance, yeast’s complex glycosylation profile can lead to intricate protein production scenarios, including hyperglycosylation issues. Consequently, Corynebacterium glutamicum emerges as a promising alternative, given its adaptability and recent advances in metabolic engineering. Although extensively used in biotechnological applications, the potential production of flavonoid and stilbenoid in engineered C. glutamicum remains largely untapped compared to E. coli. This review explores the potential of metabolic engineering in C. glutamicum for biosynthesis, highlighting its versatility as a cell factory and assessing optimization strategies for these pathways. Additionally, various metabolic engineering methods, including genomic editing and biosensors, and cofactor regeneration are evaluated, with a focus on C. glutamicum. Through comprehensive discussion, the review offers insights into future perspectives in production, aiding researchers and industry professionals in the field.

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Section: Future Perspectives and Conclusionmentioning

confidence: 99%

Metabolic Engineering of Corynebacterium glutamicum for the Production of Flavonoids and Stilbenoids

Chu,

Tran,

Pham

et al. 2024

Molecules

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The small yeast GTPase Rho5 requires specific mitochondrial outer membrane proteins for translocation under oxidative stress and interacts with the VDAC Por1

Bischof,

Schweitzer,

Schmitz

et al. 2024

European Journal of Cell Biology

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Strategies to Maintain Redox Homeostasis in Yeast Cells with Impaired Fermentation-Dependent NADPH Generation

Kwolek-Mirek,

Maslanka,

Bednarska

et al. 2024

IJMS

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Redox homeostasis is the balance between oxidation and reduction reactions. Its maintenance depends on glutathione, including its reduced and oxidized form, GSH/GSSG, which is the main intracellular redox buffer, but also on the nicotinamide adenine dinucleotide phosphate, including its reduced and oxidized form, NADPH/NADP+. Under conditions that enable yeast cells to undergo fermentative metabolism, the main source of NADPH is the pentose phosphate pathway. The lack of enzymes responsible for the production of NADPH has a significant impact on yeast cells. However, cells may compensate in different ways for impairments in NADPH synthesis, and the choice of compensation strategy has several consequences for cell functioning. The present study of this issue was based on isogenic mutants: Δzwf1, Δgnd1, Δald6, and the wild strain, as well as a comprehensive panel of molecular analyses such as the level of gene expression, protein content, and enzyme activity. The obtained results indicate that yeast cells compensate for the lack of enzymes responsible for the production of cytosolic NADPH by changing the content of selected proteins and/or their enzymatic activity. In turn, the cellular strategy used to compensate for them may affect cellular efficiency, and thus, the ability to grow or sensitivity to environmental acidification.

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The Role of Glucose-6-phosphate Dehydrogenase in the Wine Yeast Hanseniaspora uvarum

Cited by 3 publications

References 63 publications

Metabolic Engineering of Corynebacterium glutamicum for the Production of Flavonoids and Stilbenoids

Metabolic Engineering of Corynebacterium glutamicum for the Production of Flavonoids and Stilbenoids

The small yeast GTPase Rho5 requires specific mitochondrial outer membrane proteins for translocation under oxidative stress and interacts with the VDAC Por1

Strategies to Maintain Redox Homeostasis in Yeast Cells with Impaired Fermentation-Dependent NADPH Generation

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