The role of glycerol transporters in yeast cells in various physiological and stress conditions

Dušková, Michala; Borovikova, Diāna; Herynkova, Pavla; Rapoport, Alexander; Sychrová, Hana

doi:10.1093/femsle/fnu041

Cited by 37 publications

(28 citation statements)

References 38 publications

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“…Hence it appears that there are mechanisms in place that prevent the production of glycerol under such conditions. Yeast cells employ external glycerol and accumulate it because the Stl1 proton-driven glycerol uptake system is produced and active in ethanol-grown cells 26, 34 . Consistent with this, the hog1Δ mutant displayed improved growth when glycerol was supplemented to the stress medium.…”

Section: Discussionmentioning

confidence: 99%

The yeast osmostress response is carbon source dependent

Babazadeh

Lahtvee

Adiels

et al. 2017

Sci Rep

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Adaptation to altered osmotic conditions is a fundamental property of living cells and has been studied in detail in the yeast Saccharomyces cerevisiae. Yeast cells accumulate glycerol as compatible solute, controlled at different levels by the High Osmolarity Glycerol (HOG) response pathway. Up to now, essentially all osmostress studies in yeast have been performed with glucose as carbon and energy source, which is metabolised by glycolysis with glycerol as a by-product. Here we investigated the response of yeast to osmotic stress when yeast is respiring ethanol as carbon and energy source. Remarkably, yeast cells do not accumulate glycerol under these conditions and it appears that trehalose may partly take over the role as compatible solute. The HOG pathway is activated in very much the same way as during growth on glucose and is also required for osmotic adaptation. Slower volume recovery was observed in ethanol-grown cells as compared to glucose-grown cells. Dependence on key regulators as well as the global gene expression profile were similar in many ways to those previously observed in glucose-grown cells. However, there are indications that cells re-arrange redox-metabolism when respiration is hampered under osmostress, a feature that could not be observed in glucose-grown cells.

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Section: Discussionmentioning

confidence: 99%

The yeast osmostress response is carbon source dependent

Babazadeh

Lahtvee

Adiels

et al. 2017

Sci Rep

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“…This is a different feature from S. cerevisiae , where the hypoosmotic stress after the hyperosmotic stress kills a significant amount of stl1Δ cells (Duskova et al . ).…”

Section: Resultsmentioning

confidence: 97%

Stl1 transporter mediating the uptake of glycerol is not a weak point of Saccharomyces kudriavzevii's low osmotolerance

Zemančíková

Papoušková

Pérez‐Torrado

et al. 2018

Lett Appl Microbiol

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Significance and Impact of the Study: An increasing demand for food products with benefits for human health turns the attention to less-exploited nonconventional yeasts with interesting traits not found in Saccharomyces cerevisiae. Among them, Saccharomyces kudriavzevii has good potential for aroma-compound production, fermentations and other biotechnological applications, but it is less adapted to stressful industrial conditions. This report studied S. kudriavzevii relative osmosensitivity and its capacity for active glycerol uptake. The results obtained (on the activity and physiological function of S. kudriavzevii glycerol transporter) may contribute to a further engineering of this species aiming to improve its osmotolerance. AbstractSaccharomyces kudriavzevii is a nonconventional and rather osmosensitive yeast with a high potential of use in fermentation processes. To elucidate the basis of its relative osmosensitivity, the role of the STL1 gene encoding a putative glycerol uptake system was studied. Under higher osmotic pressure, the addition of a low amount of glycerol to the growth medium improved the growth of S. kudriavzevii and the expression of the STL1 gene was highly induced. Deletion of this gene decreased the strain's ability to grow in the presence of higher concentrations of salts and other solutes. Moreover, the mutant had a disturbed homeostasis of intracellular pH. Expression of the SkSTL1 gene in Saccharomyces cerevisiae complemented the osmosensitivity of the S. cerevisiae hog1D stl1D mutant, and the gene's tagging with GFP localized its product to the plasma membrane. Altogether, a deficiency in glycerol uptake did not seem to be the reason for S. kudriavzevii's low osmotolerance; its Stl1 transporter properly contributes to the regulation of intracellular pH and is crucial to its survival of osmotic stress.Letters in Applied Microbiology ISSN 0266-8254

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“…Both C. albicans and Sa. cerevisiae do not seem to be affected by the absence of the STL1 gene under non-stressed conditions (Ferreira and Lucas, 2007;Kayingo et al, 2009;Duskova et al, 2015). In Z. rouxii, the strongest osmosensitivity of stl mutants was observed when mannitol was used as a source of carbon (e.g.…”

Section: Discussionmentioning

confidence: 99%

Two glycerol uptake systems contribute to the high osmotolerance of Zygosaccharomyces rouxii

Dušková

Ferreira

Lucas

et al. 2015

Molecular Microbiology

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SummaryThe accumulation of glycerol is essential for yeast viability upon hyperosmotic stress. Here we show that the osmotolerant yeast Zygosaccharomyces rouxii has two genes, ZrSTL1 and ZrSTL2, encoding transporters mediating the active uptake of glycerol in symport with protons, contributing to cell osmotolerance and intracellular pH homeostasis. The growth of mutants lacking one or both transporters is affected depending on the growth medium, carbon source, strain auxotrophies, osmotic conditions and the presence of external glycerol. These transporters are localised in the plasma membrane, they transport glycerol with similar kinetic parameters and besides their expected involvement in the cell survival of hyperosmotic stress, they surprisingly both contribute to an efficient survival of hypoosmotic shock and to the maintenance of intracellular pH homeostasis under non-stressed conditions. Unlike STL1 in Sa. cerevisiae, the two Z. rouxii STL genes are not repressed by glucose, but their expression and activity are downregulated by fructose and upregulated by nonfermentable carbon sources, with ZrSTL1 being more influenced than ZrSTL2. In summary, both transporters are highly important, though Z. rouxii CBS 732 T cells do not use external glycerol as a source of carbon.

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The role of glycerol transporters in yeast cells in various physiological and stress conditions

Cited by 37 publications

References 38 publications

The yeast osmostress response is carbon source dependent

The yeast osmostress response is carbon source dependent

Stl1 transporter mediating the uptake of glycerol is not a weak point of Saccharomyces kudriavzevii's low osmotolerance

Two glycerol uptake systems contribute to the high osmotolerance of Zygosaccharomyces rouxii

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