The Role of GmSnRK1-GmNodH Module in Regulating Soybean Nodulation Capacity

Abstract

SnRK1 protein kinase plays hub roles in plant carbon and nitrogen metabolism. However, the function of SnRK1 in legume nodulation and symbiotic nitrogen fixation is still elusive. In this study, we identified GmNodH, a putative sulfotransferase, as an interacting protein of GmSnRK1 by yeast two-hybrid screen. The qRT-PCR assays indicate that GmNodH gene is highly expressed in soybean roots and could be induced by rhizobial infection and nitrate stress. Fluorescence microscopic analyses showed that GmNodH was colocalized with GsSnRK1 on plasma membrane. The physical interaction between GmNodH and GmSnRK1 was further verified by using split-luciferase complementary assay and pull-down approaches. In vitro phosphorylation assay showed that GmSnRK1 could phosphorylate GmNodH at Ser193. To dissect the function and genetic relationship of GmSnRK1 and GmNodH in soybean, we co-expressed the wild-type and mutated GmSnRK1 and GmNodH genes in soybean hairy roots and found that co-expression of GmSnRK1/GmNodH genes significantly promoted soybean nodulation rates and the expression levels of nodulation-related GmNF5α and GmNSP1 genes. Taken together, this study provides the first biological evidence that GmSnRK1 may interact with and phosphorylate GmNodH to synergistically regulate soybean nodulation.

Strigolactones repress nodule development and senescence in pea

“…When strigolactone biosynthesis and signaling are deficient, this additional control on the sugar regulators might be absent, hence, boosting nodule development to such an extent that there is not sufficient carbon input resulting in early nodule senescence. Indeed, a close connection between nitrate signaling and the conserved energy regulator sucrose nonfermenting 1 (SNF1)‐related protein kinase 1 has been recently proven (Wang et al., 2022) that might also play a role in nodulation in soybean (Li et al., 2023). It would be interesting to investigate which of the main sugar regulators act on NIN and might be involved in the strigolactone‐mediated regulation of nodule development.…”

Quantitative Phosphoproteomic Analysis Provides Insights into the Sodium Bicarbonate Responsiveness of Glycine max

“…Protein-protein interactions were determined by the yeast two-hybrid (Y2H) approach according to Li et al [25]. Briefly, the full-length CDSs of the candidate genes were cloned into pGBKT7 or pGADT7 vectors, respectively.…”