The Role of Green Tea Extract on the Proliferation of Human Ovarian Cancer Cells (in vitro) Study

Green tea for ovarian cancer prevention and treatment: A systematic review of the in vitro, in vivo and epidemiological studies

“…Infused green tea would also lead to EOC growth inhibition, but the very concise methodology does not allow us to generalize results from this study [23]. On the other hand, Ravindranath et al reported that ECG has very potent EOC growth-inhibitory properties on four cells lines [15].…”

“…Otherwise, the IC 50 of epicatechin gallate (ECG) in HH450 and HH639 cells were 28.95 μM and 29.59 μM, respectively [15]. Infused green tea (12.5% w/v) also demonstrated growth inhibitory effects on a human ovarian cancer cell line [23]. However, the highest dose of a nutrient mixture including various bioactive compounds and approximately 184 μM of EGCG did not induce changes in the proliferation rate of SKOV3 cells following a 24 hours treatment [25].…”

“…Using a DNA chip targeting apoptosis-related genes, Kim et al reported that EGCG downregulated p55cdc, Cyclin B1 and Cyclin A; however, these results were not statistically significant [18,19]. EGCG treatment (10-80 μg/ml; 21.8-174.5 μM) for two days induced morphological cell changes typical of apoptosis in SKOV3 cells [20] as did infused green tea in human ovarian cancer cells after 24 h of incubation [23]. Lastly, evidence linking EOC cell growth inhibition to an EGCG-induced cell cycle arrest is weak [18][19][20].…”

“…The dose-dependent inhibitory effect of EGCG or green tea on EOC cell growth was consistently reported [14][15][16][17][18][19][20][21][22][23]40,62]. The half maximum inhibitory concentrations (IC 50 ) of EGCG on cell proliferation were calculated in 14 different EOC cell lines, including SKOV3 [14,15,17,40,62] (Fig.…”

“…The expression of ET-1 and its receptor ET A R was decreased by EGCG [25] No Δ in C proliferation or C morphology SKOV3/Nutrient mixture b at 0, 10, 50, 100, 500 and 1000 μg/ml/ 24 h Effects of nutrient mixture on MMP2: see Table 3 Spinella, 2006 [27] Effects of green tea and EGCG on ET A R, ET-1, COX1, COX2 and PGE 2 : see Table 3 Spinella, 2006 [40] Growth inhibition IC 50 Effects of green tea and EGCG on ET A R, ET-1, phosphorylated p38 and p42/p44 MAPKs, AKT, MMP2, MMP9, MMP14, uPA, uPAR, VEGF: see Table 3 Wilson-Simpson, 2007 [22] Inhibition of C growth, induction of cell membrane damage only at 24 h ES-2/EGCG 50, 500 μM/24, 48, 72 h Yin, 2009 [26] No Δ in C viability SKOV3/EGCG 2, 10, 50 μM/8-20 h Effects of EGCG on phospho-AKT, HER2, Raf-1, Hsp70 and Hsp90: see Table 3 Mahboub, 2010 [23] "Ideal growth inhibition concentration": 100 μg/ml "Human ovarian cancer C"/Tea from infused green tea leaves ( [24] Effect of EGCG on MMP2: see Table 3 Yan, 2011 [21] Inhibition of C growth and induction of apoptosis in a dose-and time-dependent manner SKOV3/EGCG 20, 40, 60, 100 μg/ml/24 h or EGCG 40 μg/ml/6, 12, 24, 48, 72 h Effects of EGCG on aquaporin 5, IκBα, NF-κB: see Table 3 Yunos, 2011 [17] Growth inhibition IC 50 and green tea, both at protein and mRNA levels [27,40]. The effect of EGCG on some of the downstream effectors of ET-1 was therefore tested [27,40].…”