The role of gut microbiota in the regulation of standard metabolic rate in femalePeriplaneta americana

Abstract

Insect gut microbiota contribute significantly to host nutritional ecology. Disrupting insect gut microbial assemblages impacts nutrient provisioning functions, and can potentially affect host standard metabolic rate (SMR), a measure of host energy balance. In this study, we evaluated the effect of disrupting gut microbial assemblages on the SMR of female Periplaneta americana cockroaches fed dog food (DF, high protein/carbohydrate (p/c) ratio), and cellulose-amended dog food (CADF, 30% dog food, 70% cellulose, low p/c ratio) diets, supplemented with none, low, or high antibiotic doses. Bacterial loads decreased significantly between diet types (P = 0.04) and across antibiotic doses (P = 0.04). There was a significant diet type x antibiotic dose interaction on SMR of females on both diets (P = 0.05) by the end of the seven-day experimental period. In CADF-fed females, SMR decreased linearly with decreasing bacterial load. However, SMR of DF-fed females on the low dose was significantly higher than those in the control and high dose groups. This is interpreted as a diet-dependent response by low dose DF-fed females to the loss of nutritional services provided by gut bacteria. Severe reductions in bacterial load at high doses reduced SMR of females on both diet types. This study provides insights into the potential role of gut bacteria as modulators of host energy expenditure under varying dietary conditions.

Variation in the pH of experimental diets affects the performance ofLymantria dispar asiaticalarvae and its gut microbiota

“…Similarly, numerous investigations have been devoted to elucidating the role of intestinal microflora in mammals (Pasinetti et al, 2018;Roselli et al, 2017) and fishes (Gómez & José Luis, 2010;Zhou, Ringø, Olsen, & Song, 2018). Although studying entomic intestinal microflora remains relatively novel, its development and metabolism, even behavior is shown to be similar to the gut microbiota of the other animals (Ayayee, Muñoz-Garcia, & Keeney, 2018;Qiao, Keesey, Hansson, & Knaden, 2019;Xia et al, 2018).…”

Disruption of the microbiota affects physiological and evolutionary aspects of insecticide resistance in the German cockroach, an important urban pest

“…That is, while we and others [23] show that Blattabacterium endosymbionts do not appear to be killed by antibiotic exposure except during their transit to the developing oocyte, it is difficult to rule out sub-lethal antibiotic effects on these microbes that may impact their metabolic status. It is highly probable that the microbial community in the gut is involved in regulating metabolic rate, as in the American cockroach [50]. Because this community is efficiently targeted by doxycycline treatment, it is a more likely contributor to life history.…”

Ambient temperature‐mediated enzymic activities and intestinal microflora in Lymantria dispar larvae

“…The previous study suggested that intestinal microflora was involved in human's physical and mental health (Petersen, Skov, Thyssen, & Jensen, ; Stricoff & Robinson, ). Similarly, intestinal microflora plays diverse functions in invertebrate, such as olfactory behavior regulation (Qiao, Keesey, Hansson, & Knaden, ), metabolism (Ayayee, Muñoz‐Garcia, & Keeney, ; B. Chen et al, ), and immune (Lee, Lee, & Lee, ; Martemyanov et al, ; Xia et al, ). In addition, consistent changes of gut microbial community and host development indicated that the gut microbiota might be involved in the entomic development (B. Chen et al, ).…”

“…The previous study suggested that intestinal microflora was involved in human's physical and mental health (Petersen, Skov, Thyssen, & Jensen, 2019;Stricoff & Robinson, 2018). Similarly, intestinal microflora plays diverse functions in invertebrate, such as olfactory behavior regulation (Qiao, Keesey, Hansson, & Knaden, 2019), metabolism (Ayayee, Muñoz-Garcia, & Keeney, 2018;B. Chen et al, 2016), and immune (Lee, Lee, & Lee, 2017;Martemyanov et al, 2016;Xia et al, 2018).…”