The role of GW/P-bodies in RNA processing and silencing

Abstract

There was an error published in J. Cell Sci. 120, 1317-1323.We apologise for two errors that occurred in the online and pdf versions of this article. The printed version is correct.On p. 1317, in the Summary, the sentence 'Formation of GW bodies appears to depend on both specific protein factors and RNA, in particular, microRNA.' appeared twice. The correct version of the summary is shown below. Summary GW bodies, also known as mammalian P-bodies, are cytoplasmic foci involved in the post-transcriptional regulation of eukaryotic gene expression. Recently, GW bodies have been linked to RNA interference and demonstrated to be important for short-interfering-RNA-and microRNA-mediated mRNA decay and translational repression. Evidence indicates that both passenger and guide strands of short-interfering RNA duplexes can localize to GW bodies, thereby indicating that RNA-induced silencing complexes may be activated within these cytoplasmic centers. Work over the past few years has significantly increased our understanding of the biology of GW bodies, revealing that they are specialized cell components that spatially regulate mRNA turnover in various biological processes. Formation of GW bodies appears to depend on both specific protein factors and RNA, in particular, microRNA. Here, we propose a working model for GW body assembly in terms of its relationship to RNA interference. In this process, one or more heteromeric protein complexes accumulate in successive steps into larger ribonucleoprotein structures.On p. 1319, right column, first paragraph, the word order of the penultimate sentence was incorrect and should read:In particular, studies in Drosophila indicate that GW182 interacts with Ago1 and promotes miRNA-mediated degradation of a subset of mRNA targets (Behm-Ansmant et al., 2006).

Exogenous nucleic acids aggregate in non-P-body cytoplasmic granules when transfected into cultured cells

“…Similarly, GFP-fused DCP1 (a P-body component) was constructed and co-transfected into cultured cells with siRNA, displaying the spatial relationship between transfected siRNA and P bodies. In contrast to studies showing that transfected siRNA colocalizes with RISC and P bodies (Liu et al 2005a(Liu et al ,2005bBerezhna et al, 2006;Pauley et al, 2006;Jakymiw et al, 2007), confocal imaging as well as quantitative analysis in the present study revealed that the siRNA aggregated into cytoplasmic granules that are distinct from RISC or P bodies (Fig. 1).…”

Integrated analysis of mRNA and microRNA expression in mature neurons, neural progenitor cells and neuroblastoma cells

“…MicroRNAs bind to complementary sequences in the 3′UTRs of target mRNAs, (Bartel, 2004(Bartel, , 2009 resulting in negative regulation (transcript degradation and post-translational suppression) (Jakymiw et al, 2007;Lim et al, 2005;Wu et al, 2006). MicroRNAs may also bind to the non-coding regulatory regions in gene promoters, sometimes leading to positive regulation (transcriptional and translational activation) (Li et al, 2006;Mao et al, 2008;Place et al, 2008;Suzuki and Kelleher, 2009).…”

“…These percentages of mRNAs as predicted targets of miRNA are within the range reported in the literature (10-60%). (Friedman et al, 2009) Although translational repression is the main mechanism by which miRNA suppresses expression in mammalian cells, the suppressed target mRNA is often eventually degraded in cellular Processing bodies (P-bodies) (Jakymiw et al, 2007;Lim et al, 2005;Wu et al, 2006). Thus increases of miRNAs often lead to decreases of potential target mRNAs.…”

Update on autoantibodies in systemic sclerosis

“…Glycine/tryptophan/processing bodies Glycine/tryptophan (GW) bodies (GWBs) are 30-100 mm cytoplasmic structures containing a GW-rich mRNA binding protein GW182 (reviewed in [107]). They are sites for mRNA processing and degradation, and are important in the RNA interference (RNAi) pathway (reviewed in [107,108]).…”

“…They are sites for mRNA processing and degradation, and are important in the RNA interference (RNAi) pathway (reviewed in [107,108]). The most common diagnoses of patients with autoantibodies to components of GWBs included neuropathies ($33%), SjS ($30%), and SSc ($15%), including an overlap with PBC (R. Bhanji, T. Eystathioy, E.K.L.…”