The role of heat shock protein 70 (Hsp 70) in male infertility: is it a line of defense against sperm DNA fragmentation?

Erata, Gül Özdemirler; Toker, Nezir Yaşar; Durlanık, Özgür; Kadıoğlu, Ateş; Aktan, Gülşen; Toker, Gülçin

doi:10.1016/j.fertnstert.2007.06.021

Cited by 53 publications

(28 citation statements)

References 46 publications

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“…In our present study, a slight increase in HSP70 in transported mice was observed. The increased HSP70 expression may be a protective mechanism against apoptosis in spermatozoa (Erata et al, 2008).…”

Section: Discussionmentioning

confidence: 98%

“…HSP70 has been found to be present in the spermatozoa of mature bulls, and redistribution of the protein occurs during capacitation and the acrosome reaction (Kamaruddin et al, 2004). Significantly increased expression of HSP70 in infertile male may indicate that HSP70 is a protective protein against apoptosis in spermatozoa (Erata et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

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Differential expression of luteinizing hormone receptor, androgen receptor and heat-shock protein 70 in the testis of long-distance transported mice

et al. 2015

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ABSTRACT. Spermatogenesis, is a complex process of precisely regulated intracellular events, where it is affected by many factors. Long-distance transport of animals is one of the stressors that may influence spermatogenesis and sperm quality. The present study chose luteinizing hormone receptor (LHR), androgen receptor (AR), and heatshock protein 70 (HSP70) as our target genes to investigate their mRNA and protein expression in the testes of long-distance transported (about 1000 km) mice. Histological analysis showed that there was a reduction in the thickness of the seminiferous epithelium in the transported mice, and a significant decrease in body weight and sperm count in the epididymis was also observed. mRNA expression was determined by QPCR in the testis of transported and control mice. The levels for AR decreased significantly in transported mice. LHR and HSP70 expression in the testes of the transported mice was slightly higher than that of control mice but did not reach a significant level. A similar tendency of protein expression was also observed by Western blot analysis. The levels of LHR and HSP70 increased slightly after transportation. However, none of the changes were statistically significant compared with the control mice. In conclusion, long-distance transport has an adverse effect on reproductive organs and spermatozoa in adult mice.

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Section: Discussionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Differential expression of luteinizing hormone receptor, androgen receptor and heat-shock protein 70 in the testis of long-distance transported mice

et al. 2015

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“…Specifically, HSP70 plays an important role in spermatogenesis and sperm maturation in several species, such as mice [55] and chicken [56]. Dysregulation of hsp70 is positively correlated with infertility in human sperm samples [57]. In this study, 31 hsps were detected in our transcriptome dataset (Table S11), including small hsps, hsp40, hsp60, hsp70, and hsp90 families, which is similar to the results of studies in the male Bactrocera dorsalis [32], Eriocheir sinensis [58], and Oryctolagus cuniculus [53], which indicates the high conservation of hsp gene families among organisms.…”

Section: Heat Shock Protein Genes (Hsps)mentioning

confidence: 99%

Transcriptome Sequencing Reveals the Traits of Spermatogenesis and Testicular Development in Large Yellow Croaker (Larimichthys crocea)

Luo

Gao

Ding

et al. 2019

Genes

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Larimichthys crocea is an economically important marine fish in China. To date, the molecular mechanisms underlying testicular development and spermatogenesis in L. crocea have not been thoroughly elucidated. In this study, we conducted a comparative transcriptome analysis between testes (TES) and pooled multiple tissues (PMT) (liver, spleen, heart, and kidney) from six male individuals. More than 54 million clean reads were yielded from TES and PMT libraries. After mapping to the draft genome of L. crocea, we acquired 25,787 genes from the transcriptome dataset. Expression analyses identified a total of 3853 differentially expressed genes (DEGs), including 2194 testes-biased genes (highly expressed in the TES) and 1659 somatic-biased genes (highly expressed in the PMT). The dataset was further annotated by blasting with multi-databases. Functional genes and enrichment pathways involved in spermatogenesis and testicular development were analyzed, such as the neuroactive ligand-receptor interaction pathway, gonadotropin-releasing hormone (GnRH) and mitogen-activated protein kinase (MAPK) signaling pathways, cell cycle pathway, and dynein, kinesin, myosin, actin, heat shock protein (hsp), synaptonemal complex protein 2 (sycp2), doublesex-and mab-3-related transcription factor 1 (dmrt1), spermatogenesis-associated genes (spata), DEAD-Box Helicases (ddx), tudor domain-containing protein (tdrd), and piwi genes. The candidate genes identified by this study lay the foundation for further studies into the molecular mechanisms underlying testicular development and spermatogenesis in L. crocea.Genes 2019, 10, 958 2 of 22 remains for somatic growth; these changes eventually reduce the culture benefit of L. crocea [3] and hinder the conservation of its germplasm resources. Therefore, the molecular mechanisms underlying reproduction regulation must be investigated, and the genes involved in gametogenesis and gonadal development must be identified, to provide a theoretical reference for further studies on the above problems in L. crocea.Directly correlated with sexual maturation and reproduction, in various animal species, including L. crocea, the testis is an essential basic component of the animal reproductive system and responsible for the production of male gametes via spermatogenesis [4]. During the process of spermatogenesis, diploid spermatogonia slowly evolve into many highly specialized spermatozoa through mitosis, meiosis, and spermiogenesis. Stringent spatial and temporal expression of genes during both transcriptional and translational processes are fundamental to ensure the precise processes of spermatogenesis [5]. Previous studies have focused on the reproductive and developmental biology of L. crocea based on anatomical/histological aspects [6,7], whereas few studies have focused on genes related to spermatogenesis and testicular development. To the best of our knowledge, only a small number of reproduction-related genes have been identified in L. crocea [8][9][10][11]. To further comprehensively explore the mol...

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“…This protein changes its distribution patterns during capacitation and acrosome reaction [65] and is involved in spermatogenesis, fertilisation and post-fertilisation events [66]–[68]. Up to now, studies relating HSP70 and sperm quality have been focused on the amounts of this protein and its correlation with sperm quality parameters, heat-stress response and thermotolerance of freshly ejaculated rather than FT spermatozoa [23], [66], [69]. However, the main finding of our work is that the involvement of this protein in boar-sperm cryotolerance seems to occur, at least in part, via signal transduction pathways that involve phosphorylation-related mechanisms, as confirmed by our immunoprecipitation studies and in a similar fashion to that observed in osmotic stress response of rhesus macaque sperm [17].…”

Section: Discussionmentioning

confidence: 99%

The Increase in Phosphorylation Levels of Serine Residues of Protein HSP70 during Holding Time at 17°C Is Concomitant with a Higher Cryotolerance of Boar Spermatozoa

et al. 2014

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Boar-sperm cryopreservation is not usually performed immediately after semen collection, but rather a holding time (HT) of 4 h–30 h at 17°C is spent before starting this procedure. Taking this into account, the aim of this study was to go further in-depth into the mechanisms underlying the improving effects of HT at 17°C on boar-sperm cryotolerance by evaluating the effects of two different HTs (3 h and 24 h) on overall boar-sperm function and survival before and after cryopreservation. Given that phospho/dephosphorylation mechanisms are of utmost importance in the overall regulation of sperm function, the phosphorylation levels of serine residues (pSer) in 30 different sperm proteins after a 3 h- or 24 h-HT period were also assessed. We found that a HT of 24 h contributed to a higher sperm resistance to freeze-thawing procedures, whereas mini-array protein analyses showed that a HT of 24 h induced a significant (P<0.05) increase in pSer (from 100.0±1.8 arbitrary units in HT 3 h to 150.2±5.1 arbitrary units in HT 24 h) of HSP70 and, to a lesser extent, in protein kinases GSK3 and total TRK and in the cell-cycle regulatory protein CDC2/CDK1. In the case of HSP70, this increase was confirmed through immunoprecipation analyses. Principal component and multiple regression analyses indicated that a component explaining a percentage of variance higher than 50% in sperm cryotolerance was significantly correlated with pSer levels in HSP70. In addition, from all the parameters evaluated before freeze-thawing, only pSer levels in HSP70 resulted to be able to predict sperm cryotolerance. In conclusion, our results suggest that boar spermatozoa modulate its function during HT, at least partially, by changes in pSer levels of proteins like HSP70, and this is related to a higher cryotolerance.

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The role of heat shock protein 70 (Hsp 70) in male infertility: is it a line of defense against sperm DNA fragmentation?

Cited by 53 publications

References 46 publications

Differential expression of luteinizing hormone receptor, androgen receptor and heat-shock protein 70 in the testis of long-distance transported mice

Differential expression of luteinizing hormone receptor, androgen receptor and heat-shock protein 70 in the testis of long-distance transported mice

Transcriptome Sequencing Reveals the Traits of Spermatogenesis and Testicular Development in Large Yellow Croaker (Larimichthys crocea)

The Increase in Phosphorylation Levels of Serine Residues of Protein HSP70 during Holding Time at 17°C Is Concomitant with a Higher Cryotolerance of Boar Spermatozoa

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