The role of heat shock proteins in inflammatory injury induced by cold stress in chicken hearts

Abstract

The aim of this study was to investigate the effects of cold stress on the expression levels of heat shock proteins (Hsps90, 70, 60, 40, and 27) and inflammatory factors (iNOS, COX-2, NF-κB, TNF-α, and PTGEs) and oxidative indexes in hearts of chickens. Two hundred forty 15-day-old male chickens were randomly divided into 12 groups and kept at the temperature of 12±1°C for acute and chronic cold stress. There were one control group and five treatment groups for acute cold stress, three control groups, and three treatment groups for chronic cold stress. After cold stress, malondialdehyde level increased in chicken heart; the activity of superoxide dismutase and glutathione peroxidase in the heart first increased and then decreased. The inflammatory factors mRNA levels were increased in cold stress groups relative to control groups. The histopathological analysis showed that heart tissues were seriously injured in the cold stress group. Additionally, the mRNA levels of Hsps (70, 60, 40, and 27) increased significantly (P<0.05) in the cold stress groups relative to the corresponding control group. Meanwhile, the mRNA level and protein expression of Hsp90 decreased significantly (P<0.05) in the stress group, and showed a gradually decreasing tendency. These results suggested that the levels of inflammatory factors and Hsps expression levels in heart tissues can be influenced by cold stress. Hsps commonly played an important role in the protection of the heart after cold stress.

Effects of Arsenic Trioxide Exposure on Heat Shock Protein Response in the Immune Organs of Chickens

“…Hsp40 cooperates with Hsp70 to facilitate protein folding. Zhao et al reported that Hsp40 increased in chicken hearts in cold-stress groups relative to control groups [43]. In our study, the mRNA level of Hsp40 increased significantly (Fig.…”

Expression analysis of NOS family and HSP genes during thermal stress in goat (Capra hircus)

“…Probably, the most pivotal enzymes involved in maintaining inflammation are the inducible enzymes: iNOS and cyclooxygenase-2 (COX-2), which are responsible for the catalysis of nitric oxide (NO) and prostaglandin E2 (PGE2), respectively (Surh et al 2001). In a recent study, Zhao et al (2013) showed the increased (P<0.05) iNOS mRNA levels of heart tissue in acute cold stressed (1 to 24 h) and chronic cold stressed (5 to 20 days) groups compared with control groups. Teshfam et al (2006) indicated that cold exposure could significantly increase eNOS and iNOS gene expressions in the lungs of broiler chickens.…”

Influence of seasonal changes on physiological variables, haematology and serum thyroid hormones profile in male Red Sokoto and Sahel goats

“…Similarly, these seasonal differences also affect the availability of feed since the growth of plants occur, mainly during the RAS, and the animals must be supplemented with hay and/or concentrate during the dry seasons (Scogings et al 2015). High (Gaughan et al 2013) or low (Zhao et al 2013) ambient temperatures (ATs) as an aftermath of seasonal changes are well known to induce stress, with negative consequences on the physiology and productivity of livestock. Over the years, scientists have put intense efforts into understanding how domestic animals respond to climate stressors.…”