The role of heterocytes in the physiology and ecology of bloom-forming harmful cyanobacteria

Yema, Lilen; Litchman, Elena; Pinto, Paula de Tezanos

doi:10.1016/j.hal.2016.11.007

Cited by 31 publications

(19 citation statements)

References 39 publications

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“…Furthermore, Dolichospermum had heterocytes at the start of these two experiments, further suggesting that ambient nitrate was not available, because Dolichospermum will not produce heterocytes if it is growing on nitrate (Yema et al, 2016). (Callieri, Bertoni, Contesini, & Bertoni, 2014;Carey, Weathers, & Cottingham, 2008;Salmaso, Capelli, Shams, & Cerasino, 2015).…”

Section: Discussionmentioning

confidence: 98%

“…Increased Fe alleviated the Fe limitation of green algae and diatoms, and allowed them to increase chl a concentration. Furthermore, Dolichospermum had heterocytes at the start of these two experiments, further suggesting that ambient nitrate was not available, because Dolichospermum will not produce heterocytes if it is growing on nitrate (Yema et al., 2016). The colimitations of Fe and N could have selected for Dolichospermum dominance over eukaryotic algae and Microcystis in the central basin of Lake Erie.…”

Section: Discussionmentioning

confidence: 99%

“…Diazotrophic cyanobacteria have an additional need for Fe as a co-factor for the nitrogenase enzyme used in N fixation. Nitrogen fixation occurs in specialised cells called heterocytes (Yema, Litchman, & de Tezanos, 2016), and heterocyte differentiation is dependent on boron (B) for the synthesis of the cell wall (Bonilla, Garcia-González, & Mateo, 1990). Furthermore, B is required by diatoms for cell wall formation (Lewin, 1966).…”

Section: Introductionmentioning

confidence: 99%

“…The goal of this research was to provide insights into why diazotrophic cyanobacterium blooms in the N‐rich, P‐poor waters of the central basin of Lake Erie, with specific attention to the role of nutrients beside P constraining phytoplankton growth, ambient nitrate assimilation, and heterocyte number (heterocyte expression in Dolichospermum is a good proxy for N‐fixation; Yema et al [2016]). Nutrient enrichment bioassays were conducted with enrichments of P and combination enrichments of P, N (nitrate and ammonium), Fe, Mo, and B.…”

Section: Introductionmentioning

confidence: 99%

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Nutrient addition effects on chlorophyll a, phytoplankton biomass, and heterocyte formation in Lake Erie’s central basin during 2014–2017: Insights into diazotrophic blooms in high nitrogen water

et al. 2020

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1. Phosphorus (P) usually is the primary limiting nutrient of phytoplankton biomass, but attention towards nitrogen (N) and trace nutrients, such as iron (Fe), has surfaced. Additionally, N-fixing cyanobacterial blooms have been documented to occur in N-rich, P-poor waters, which is counterintuitive from the paradigm that low N and high P promotes blooms. For example, Lake Erie's central basin has Dolichospermum blooms when nitrate concentrations are high, which raises questions about which nutrient(s) are selecting for Dolichospermum over other phytoplankton and why an N-fixer is present in high N waters? 2. We conducted a 4-year (2014-2017) study in Lake Erie's central basin to determine which nutrient (P, N, or trace nutrients such as Fe, molybdenum [Mo], and boron [B]) constrained chlorophyll concentration, phytoplankton biovolume, and nitrate assimilation using nutrient enrichment bioassays. The enriched lake water was incubated in 1-L bottles in a growth chamber programmed at light and temperatures of in situ conditions for 4-7 days. We also quantified heterocytes when N-fixing cyanobacteria were present. 3. Compared to the non-enriched control, the P-enriched (+P) treatment had significantly higher chlorophyll and phytoplankton biovolume in c. 75% of experiments. Combination enrichments of P with ammonium-N, nitrate-N, Fe, Mo, and B were compared to the +P treatment to determine secondary limitations. +P and ammonium-N and +P nitrate-N resulted in higher chlorophyll in 50% of experiments but higher phytoplankton biovolume in only 25% of experiments. These results show that P was the primary limiting nutrient, but there were times when N was secondarily limiting. 4. Chlorophyll concentration indicated N secondary limitation in half of the experiments, but biovolume indicated only N secondary limitation in 25% of the experiments. To make robust conclusions from nutrient enrichment bioassays, both chlorophyll and phytoplankton biovolume should be measured. 5. The secondary effects of Fe, Mo, and B on chlorophyll were low (<26% of experiments), and no secondary effects were observed on phytoplankton biovolume | 2155 CHAFFIN et Al.

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Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Nutrient addition effects on chlorophyll a, phytoplankton biomass, and heterocyte formation in Lake Erie’s central basin during 2014–2017: Insights into diazotrophic blooms in high nitrogen water

et al. 2020

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“…A previous study has suggested that heterocyst-to-vegetative cell ratio could be an adequate indicator of N fixation in cyanobacteria [ 36 ]. An increased heterocyst frequency exhibited by A. variabilis in this study indicated that N fixation occurred to meet the N demand after its adaptation to various N-limiting conditions.…”

Section: Discussionmentioning

confidence: 99%

Heterocyst Development and Diazotrophic Growth of Anabaena variabilis under Different Nitrogen Availability

Zulkefli

Hwang

2020

Life

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Nitrogen is globally limiting primary production in the ocean, but some species of cyanobacteria can carry out nitrogen (N) fixation using specialized cells known as heterocysts. However, the effect of N sources and their availability on heterocyst development is not yet fully understood. This study aimed to evaluate the effect of various inorganic N sources on the heterocyst development and cellular growth in an N-fixing cyanobacterium, Anabaena variabilis. Growth rate, heterocyst development, and cellular N content of the cyanobacteria were examined under varying nitrate and ammonium concentrations. A. variabilis exhibited high growth rate both in the presence and absence of N sources regardless of their concentration. Ammonium was the primary source of N in A. variabilis. Even the highest concentrations of both nitrate (1.5 g L−1 as NaNO3) and ammonium (0.006 g L−1 as Fe-NH4-citrate) did not exhibit an inhibitory effect on heterocyst development. Heterocyst production positively correlated with the cell N quota and negatively correlated with vegetative cell growth, indicating that both of the processes were interdependent. Taken together, N deprivation triggers heterocyst production for N fixation. This study outlines the difference in heterocyst development and growth in A. variabilis under different N sources.

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Drivers of cyanobacteria dominance, composition and nitrogen fixing behavior in a shallow lake with alternative regimes in time and space, Laguna del Sauce (Maldonado, Uruguay)

et al. 2018

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Laguna del Sauce, one of the main drinking water sources in Uruguay, is an eutrophic shallow lake with high temporal variation of inorganic turbidity caused by extreme wind events. During low turbidity periods, high phytoplankton biomass can be reached, frequently associated to cyanobacteria blooms, which can cause interferences in the water supply. In this study, we assessed the environmental drivers of cyanobacteria dominance, composition, and nitrogen-fixation behavior. For this, we analyzed the spatial and temporal phytoplankton composition, physical and chemical variables performing weekly samplings during two summers: 2015-2016 and 2016-2017. When inorganic turbidity was high (above 30 NTU), phytoplankton biomass was controlled, below this threshold, temperature, secchi depth and nutrients played key factors controlling cyanobacteria biomass and composition. Blooms of N 2 -fixing cyanobacteria (Dolichospermum crassum, Aphanizomenon gracile, and Cuspidothrix issatschenkoi) were promoted by low N:P ratios (average 11.5) and wide TN range (286-1300 lg l -1 ). Non-heterocystous cyanobacteria blooms occurred above TN

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The role of heterocytes in the physiology and ecology of bloom-forming harmful cyanobacteria

Cited by 31 publications

References 39 publications

Nutrient addition effects on chlorophyll a, phytoplankton biomass, and heterocyte formation in Lake Erie’s central basin during 2014–2017: Insights into diazotrophic blooms in high nitrogen water

Nutrient addition effects on chlorophyll a, phytoplankton biomass, and heterocyte formation in Lake Erie’s central basin during 2014–2017: Insights into diazotrophic blooms in high nitrogen water

Heterocyst Development and Diazotrophic Growth of Anabaena variabilis under Different Nitrogen Availability

Drivers of cyanobacteria dominance, composition and nitrogen fixing behavior in a shallow lake with alternative regimes in time and space, Laguna del Sauce (Maldonado, Uruguay)

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