The Role of Histo-Blood Group Antigens and Microbiota in Human Norovirus Replication in Zebrafish Larvae

Cuvry, Arno; Gozalbo-Rovira, Roberto; Strubbe, Dufie; Neyts, Johan; Witte, Peter de; Rodrı́guez-Dı́az, Jesús; Rocha‐Pereira, Joana

doi:10.1128/spectrum.03157-22

Cited by 5 publications

(3 citation statements)

References 85 publications

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“…In addition, the zebrafish larvae model has been employed to investigate the role of HBGAs and the host microbiota during HuNoV infection. The findings showed that successful HuNoV infection in zebrafish larvae depends on the presence of terminal fucoses, an integral part of HBGAs [120]. This aligns with infections observed in humans and in other recently established in vitro HuNoV models, emphasizing the consistent requirement for fucose residues on intestinal cells and suggesting a shared entry mechanism for HuNoV infection in zebrafish.…”

Section: Zebrafish Model For Detecting Infectious Human Norovirussupporting

confidence: 83%

“…This aligns with infections observed in humans and in other recently established in vitro HuNoV models, emphasizing the consistent requirement for fucose residues on intestinal cells and suggesting a shared entry mechanism for HuNoV infection in zebrafish. Additionally, this study also demonstrated that neither the zebrafish microbiota nor the presence of HBGA-expressing bacteria in the zebrafish intestine amplified HuNoV replication during the early larval stages of their development [120]. This differs from the results seen in B-cells, where the presence of HBGA-expressing enteric bacteria was required for HuNoV infection [108].…”

Section: Zebrafish Model For Detecting Infectious Human Noroviruscontrasting

confidence: 77%

“…This model has demonstrated the replication of several strains belonging to the GII genogroup, along with a single strain from the GI genogroup (GI.7), by microinjecting filtered stool samples into the yolk of 3-days-post-fertilization (dpf) zebrafish larvae. The zebrafish larvae model has also been used in evaluating the efficacy of small molecule inhibitors, elucidating the role of HBGAs and microbiota in HuNoV infection, and assessing disinfection treatments for HuNoV inactivation [119][120][121][122]. More recently, a zebrafish embryo model with enhanced efficiency and robustness, compared to the larvae model, has been reported.…”

Section: Cell Culture and Animal Models Available For Detecting Infec...mentioning

confidence: 99%

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Improving the Detection and Understanding of Infectious Human Norovirus in Food and Water Matrices: A Review of Methods and Emerging Models

Chandran,

Gibson

2024

Viruses

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Human norovirus (HuNoV) is a leading global cause of viral gastroenteritis, contributing to numerous outbreaks and illnesses annually. However, conventional cell culture systems cannot support the cultivation of infectious HuNoV, making its detection and study in food and water matrices particularly challenging. Recent advancements in HuNoV research, including the emergence of models such as human intestinal enteroids (HIEs) and zebrafish larvae/embryo, have significantly enhanced our understanding of HuNoV pathogenesis. This review provides an overview of current methods employed for HuNoV detection in food and water, along with their associated limitations. Furthermore, it explores the potential applications of the HIE and zebrafish larvae/embryo models in detecting infectious HuNoV within food and water matrices. Finally, this review also highlights the need for further optimization and exploration of these models and detection methods to improve our understanding of HuNoV and its presence in different matrices, ultimately contributing to improved intervention strategies and public health outcomes.

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Section: Zebrafish Model For Detecting Infectious Human Norovirussupporting

confidence: 83%

Section: Zebrafish Model For Detecting Infectious Human Noroviruscontrasting

confidence: 77%

Section: Cell Culture and Animal Models Available For Detecting Infec...mentioning

confidence: 99%

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Improving the Detection and Understanding of Infectious Human Norovirus in Food and Water Matrices: A Review of Methods and Emerging Models

Chandran,

Gibson

2024

Viruses

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Utilizing Zebrafish Embryos for Replication of Tulane Virus: A Human Norovirus Surrogate

Chandran,

Gibson

2024

Food Environ Virol

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The zebrafish larvae/embryo model has been shown to support the replication of seven strains (G1.7[P7], GII.2[P16], GII.3[P16], GII.4[P4], GII.4[P16], GII.6[P7], and GII.17[P13]) of human norovirus (HuNoV). However, due to challenges in consistently obtaining HuNoV-positive stool samples from clinical sources, evaluating HuNoV surrogates in this model is highly valuable. This study assesses the potential of zebrafish embryos and larvae as a model for Tulane virus (TuV) replication. Three infection methods were examined: microinjection, immersion, and feeding. Droplet digital PCR was used to quantify viral RNA across all three infection methods. Microinjection of 3 nL of TuV into zebrafish embryos (< 6-h post-fertilization) resulted in significant replication, with viral RNA levels reaching 6.22 logs at 4-day post-infection. In contrast, the immersion method showed no replication after immersing 4-day post-fertilization (dpf) larvae in TuV suspension for 6 h. Similarly, no replication was observed with the feeding method, where Paramecium caudatum loaded with TuV were fed to 4 dpf larvae. The findings indicate that the zebrafish embryo model supports TuV replication through the microinjection method, suggesting that TuV may serve as a useful surrogate for studying HuNoV pathogenesis. Additionally, TuV can be utilized in place of HuNoV in method optimization studies using the zebrafish embryo model, circumventing the limited availability of HuNoV.

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Zebrafish as a model organism for virus disease research: Current status and future directions

Sofyantoro,

Sendi Priyono,

Indah Septriani

et al. 2024

Heliyon

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The Role of Histo-Blood Group Antigens and Microbiota in Human Norovirus Replication in Zebrafish Larvae

Cited by 5 publications

References 85 publications

Improving the Detection and Understanding of Infectious Human Norovirus in Food and Water Matrices: A Review of Methods and Emerging Models

Improving the Detection and Understanding of Infectious Human Norovirus in Food and Water Matrices: A Review of Methods and Emerging Models

Utilizing Zebrafish Embryos for Replication of Tulane Virus: A Human Norovirus Surrogate

Zebrafish as a model organism for virus disease research: Current status and future directions

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