The Role of HMGB1 in Pial Arteriole Dilating Reactivity following Subarachnoid Hemorrhage in Rats

Xu, Huiping; Changyaleket, Benjarat; Vályi-Nagy, Tibor; Dull, Randal O.; Pelligrino, Dale A.; Schwartz, David E.; Chong, Zhao Zhong

doi:10.1159/000452412

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“…Interestingly, a previous study reported that H 2 only improves neuronal apoptosis at 24 h after SAH, but not 48 h. However, the present study found significant improvements in neuronal apoptosis in the vmPFC, which was different from previous studies ( 10 , 25 ). As indicated by a previous study ( 49 ), 7 day post-SAH was the most severe period of cerebral vasospasm after SAH. In the present study, it was noted that there was no change of cerebral blood flow indicated by CBF-ASL after H 2 administration 7 day post-SAH, which was consistent with previous study ( 50 ).…”

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confidence: 56%

Hydrogen gas post‑conditioning alleviates cognitive dysfunction and anxiety‑like behavior in a rat model of subarachnoid hemorrhage

et al. 2021

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Subarachnoid hemorrhage (SAH) results in high rates of mortality and lasting disability. Hydrogen gas (H 2 ) is an antioxidant with demonstrated neuroprotective efficacy. The present study examined the therapeutic efficacy of H 2 inhalation on early brain injury following experimental SAH in rats and the potential underlying molecular mechanisms. The rats were randomly separated into three groups (n=36 per group): Sham, SAH and SAH + H 2 . Endovascular perforation of the right internal carotid artery was used to establish SAH. After perforation, rats in the SAH + H 2 group inhaled 2.9% H 2 with regular oxygen for 2 h. Then, 24 h post-SAH, TUNEL staining was used to detect apoptotic neurons, and both immunostaining and western blotting were conducted to examine changes in p38 MAPK activity and the expression levels of apoptotic regulators (Bcl-2, Bax and cleaved caspase-3) in the ventromedial prefrontal cortex. Then, 30 day post-SAH, Nissl staining was performed to detect neuronal injury, brain MRI was conducted to detect gross changes in brain structure and metabolism, the open field test was used to assess anxiety and the novel object recognition test was performed to assess memory. H 2 inhalation following experimental SAH stabilized brain metabolites, improved recognition memory and reduced anxiety-like behavior, the neuronal apoptosis rate, phosphorylated p38 MAPK expression, cleaved caspase-3 expression and the Bax/Bcl-2 ratio. Collectively, the present results suggested that H 2 inhalation can alleviate SAH-induced cognitive impairment, behavioral abnormalities and neuronal apoptosis in rats, possibly via inhibition of the p38 MAPK signal pathway.

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