The Role of Host‐derived Dentinal Matrix Metalloproteinases in Reducing Dentin Bonding of Resin Adhesives

Abstract

Dentin matrix metalloproteinases (MMPs) are a family of host-derived proteolytic enzymes trapped within mineralized dentin matrix, which have the ability to hydrolyze the organic matrix of demineralized dentin. After bonding with resins to dentin there are usually some exposed collagen fibrils at the bottom of the hybrid layer owing to imperfect resin impregnation of the demineralized dentin matrix.Exposed collagen fibrils might be affected by MMPs inducing hydrolytic degradation, which might result in reduced bond strength.Most MMPs are synthesized and released from odontoblasts in the form of proenzymes, requiring activation to degrade extracellular matrix components. Unfortunately, they can be activated by modern self-etch and etch-and-rinse adhesives. The aim of this review is to summarize the current knowledge of the role of dentinal host-derived MMPs in dentin matrix degradation. We also discuss various available MMP inhibitors, especially chlorhexidine, and suggest that they could provide a potential pathway for inhibiting collagen degradation in bonding interfaces thereby increasing dentin bonding durability.

Pulpa perforasyonu ve kanama durdurucu bir ajanın kendinden pürüzlendirmeli bir adeziv sistemin dentine bağlanma dayanımı üzerine etkisi

“…26,27 MMPS'ler adeziv sitemlerle aktive olabilmektedirler ve hibrit tabakanın alt kısımlarında kalabilen rezin infilitre olamamış kollajen fibrillerin degredasyonuna neden olarak bağlantının zamanla bozulmasına ve bağlanma dayanımının düşmesine neden olabilmektedirler. [28][29][30] CPB'nin içindeki MDPB kollajen fibrillerin bu enzimlerle degredasyonunu inhibe ederek bağlanma dayanımının stabilitesine yardımcı olmuş olabilir.…”

Effects of matrix metalloproteinase enzyms on adhesive bonding

“…yaptıkları çalışmada hibrit tabakasında gerçekleşen bozulmayı incelemişler ve hibrit tabakasında MMP-2 ile MMP-9 aktivitesini göstermişle-rdir. 16 19 MMP'ler, diş gelişimi sırasında mineralize dentin matriksi içinde bulunur. Restoratif işlemler sırasında MMP enzimlerinin ortaya çıkması, dentin-adeziv bağlanmasında başarısızlık sebebi olarak görülmektedir.…”

Evaluation of Hybrid Layer and Bonding Interface after Water Storage with and without the Usage of 2% Chlorhexidine: A Scanning Electron Microscope Study

“…1 Long-term dentin bonding is threatened by the disaggregation of the hybrid layer owing mainly to the activation of dentin MMPs. 3 Host-derived MMPs, found both in saliva and etched dentin, have been shown to be involved in the degradation of the unprotected collagen fibrils within the hybrid layer. 3 These proteases are secreted by odontoblasts during dentinogenesis and remain inactive within the dentin extracellular matrix.…”

“…3 Host-derived MMPs, found both in saliva and etched dentin, have been shown to be involved in the degradation of the unprotected collagen fibrils within the hybrid layer. 3 These proteases are secreted by odontoblasts during dentinogenesis and remain inactive within the dentin extracellular matrix. The acidic environment, resulting from adhesive systems or the biologic carious process, activates different dentinal MMPs.…”

“…7 Besides its antimicrobial properties, CHX is applied to treat dentin before the use of etch-and-rinse adhesives to reduce the breakdown of collagen fibrils. 3 The CHX has been applied in different sequences, including before etching, after etching (with or without rinsing), or CHX-containing phosphoric acid. Even at low concentration (0.2%), CHX functions as MMPs inhibitor that can prevent bond degradation of collagen and disintegration of the bonding interface.…”