The Role of Human Umbilical Vein Endothelial Cells in Osteogenic Differentiation of Dental Follicle-Derived Stem Cells in In Vitro Co-cultures

Abstract

Angiogenesis and vascularization are essential for the growth and survival of most tissues. Engineered bone tissue requires an active blood vessel network for survival and integration with mature host tissue. Angiogenesis also has an effect on cell growth and differentiation in vitro. However, the effect of angiogenic factors on osteoprogenitor cell differentiation remains unclear. We studied the effects of human umbilical vein endothelial cells (HUVECs) on osteogenic differentiation of dental follicle-derived stem cells (DFSCs) in vitro by co-culturing DFSCs and HUVECs. Cell viability, based on metabolic activity and DNA content, was highest for co-cultures with a DFSC/HUVEC ratio of 50:50 in a 1:1 mixture of mesenchymal stem cell growth medium and endothelial cell growth medium. Osteoblastic and angiogenic phenotypes were enhanced in co-cultures with a DFSC/HUVEC ratio of 50:50 compared with DFSC monocultures. Increased expression of angiogenic phenotypes and vascular endothelial growth factor (VEGF) levels were observed over time in both 50:50 DFSC/HUVEC co-cultures and DFSC monocultures during culture period. Our results showed that increased angiogenic activity in DFSC/HUVEC co-cultures may stimulate osteoblast maturation of DFSCs. Therefore, the secretion of angiogenic factors from HUVECs may play a role in the osteogenic differentiation of DFSCs.

Human Umbilical Vein Endothelial Cells (HUVECs) Co-Culture with Osteogenic Cells: From Molecular Communication to Engineering Prevascularised Bone Grafts

“…This study also suggested that a HUVECs:MSCs co-culture ratio of 1:1 is the best combination to obtain both osteogenic and angiogenic differentiation. These results are consistent with that obtained in the other studies, where 1:1 ratio provided robust and stable vascular networks while enabling bone-like tissue formation [15,117].…”

AFF4 regulates osteogenic differentiation of human dental follicle cells

“…Given their remarkable properties, DFCs have been considered pivotal seed cells in periodontal tissue engineering and hopefully provide a novel approach to regeneration therapy. 7,28 Previous studies have emphasized the accurate transfer of genetic information, which is essential for life. RNA Pol II can accelerate translation by the enzymatic machinery to transcribe protein-coding genes into mRNA.…”

Isogenic-Induced Endothelial Cells Enhance Osteogenic Differentiation of Mesenchymal Stem Cells on Silk Fibroin Scaffold

“…However, there was no significant difference of osteogenic differentiation seen between predifferentiated MSC co-culture group and nonpredifferentiated MSC (data not shown). Therefore, it was deduced that osteogenic differentiation of MSCs before co-culture does not have any effect on their metabolic activity, which has been reported earlier [63,64]. This shows that osteogenic preinduction of MSCs before co-culture did not help in increase of osteogenic differentiation.…”