The role of hydrogen peroxide in chitosan-induced resistance to osmotic stress in rice (Oryza sativa L.)

Pongprayoon, Wasinee; Roytrakul, Sittiruk; Pichayangkura, Rath; Chadchawan, Supachitra

doi:10.1007/s10725-013-9789-4

Cited by 97 publications

(67 citation statements)

References 48 publications

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“…Four-day-old barley seedlings were transferred to a hydroponic growth medium containing either 0 or 360 mM mannitol for 0, 6, 12, 24 and 48 h, and the total protein including membrane protein was isolated from the roots. Solubilized samples were subjected to SD-PAGE and detected with anti-PIP1 antibody (a) or with anti-PIP2;1 antibody (b) previously proposed to be involved in the mechanisms of salinity/osmotic tolerance (Fedina et al 2009;Pongprayoon et al 2013). There are two possibilities, (1) aquaporins are not included in a pathway induced by H 2 O 2 to enhance salinity/osmotic tolerances, or (2) aquaporins are involved in a pathway induced by H 2 O 2 to enhance salinity/osmotic tolerances via translational or post-translational (but not transcriptional) regulation of PIPs activity by H 2 O 2 .…”

Section: Resultsmentioning

confidence: 99%

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Osmotic stress decreases PIP aquaporin transcripts in barley roots but H2O2 is not involved in this process

et al. 2014

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Previous reports indicate that salt stress reduces the root hydraulic conductance and the expression of plasmamembrane-type aquaporins (PIPs). As a molecular mechanism for this phenomenon, the present study found evidence that the osmotic component, but probably not an ion-specific component, decreases PIP transcripts. Eight of ten PIP transcripts were reduced to less than half by 360 mM mannitol treatment for 12 h in comparison with control samples. A large decrease of HvPIP2;1 protein was also recorded. This reduction of both transcripts and proteins of HvPIP2s should be physiologically effective for preventing or reducing dehydration at an initial phase of severe salt/osmotic stress. Root cell sap osmolality increased from 278 to 372 mOsm 24 h after 360 mM mannitol treatment. These steps can secure survival and growth recovery with water reabsorption in barley. Our data also suggest that H2O2 seems not to be the main cause of osmotic stress-induced transcriptional down-regulation within the concentrations (20-500 μM) and time periods (24 h) examined, although H2O2 was previously proposed to be involved in the mechanisms of salinity/osmotic tolerance.

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Section: Resultsmentioning

confidence: 99%

“…In addition, the amounts of HvPIP2 transcripts were determined after treatment with hydrogen peroxide (H 2 O 2 ) as this has been suggested to influence root water transport activity (Rhee et al 2007;Boursiac et al 2008;Benabdellah et al 2009) or salinity/osmotic tolerance (Fedina et al 2009;Pongprayoon et al 2013).…”

Section: Introductionmentioning

confidence: 99%

Osmotic stress decreases PIP aquaporin transcripts in barley roots but H2O2 is not involved in this process

et al. 2014

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“…Chitosan-induced drought tolerance was also demonstrated in the 'LPT123' rice line, although the same chitosan treatment did not induce drought tolerance in the somaclonal variegated 'LPT123-TC171' line. H 2 O 2 production after chitosan treatment was important for the induction of drought tolerance (Pongprayoon et al, 2013). Seed soaking with an appropriate chitosan concentration induced salt resistance in isabgol (Plantago ovata Forsk; Mahdavi, 2013), while seed priming with chitosan increased chill tolerance in maize (Guan et al, 2009) by enhancing the germination index and germination time at low temperatures, and producing greater seedling growth in treated seeds compared with untreated seeds.…”

Section: Physiological Biochemical and Growth Effectsmentioning

confidence: 99%

Biostimulant activity of chitosan in horticulture

Pichyangkura

Chadchawan

2015

Scientia Horticulturae

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“…Smaller (as in lower M W ) molecules of chitosan showed a greater induction of H 2 O 2 production than larger ones leading to a more effective triggering of defense responses in rice (Lin et al 2005). In addition, H 2 O 2 production was suggested to be required for the chitosan induced drought tolerance (Pongprayoon et al 2013). An oxidative burst together with rapid H 2 O 2 synthesis is a common response of plants to pathogens, elicitors and stresses.…”

Section: Chitosan Elicited Oxidative Burst and Detoxification Systemmentioning

confidence: 99%

“…As a consequence, the ROS scavenging system was activated in a chitosan dosedependent manner (Yusupova et al 2005). The induction of H 2 O 2 by chitosan was reported to be the crucial component in the chitosan stimulation of rice growth during drought stress (Pongprayoon et al 2013).…”

Section: Introductionmentioning

confidence: 99%

Chitosan enhances rice seedling growth via gene expression network between nucleus and chloroplast

et al. 2014

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Chitosan, a partially deacetylated form of the natural and biodegradable biopolymer chitin, has been used as plant growth promoter in agriculture. The aim of this work was to investigate the growth promoting responses induced by chitosan at the physiological and molecular level in rice (Oryza sativa L.) seedlings. The combination of the degree of deacetylation (DD), molecular weight and concentration of chitosan had differing effects on the rice seedling growth. For the best enhancement, oligomeric chitosan with an 80 % DD applied at 40 mg/L significantly enhanced the vegetative growth, in terms of the leaf and root fresh weights and dry weights of rice seedlings compared to the control. At the proteomics level, of the 352 rice leaf proteins that could be resolved using the Multi Experiment Viewer software, 105 showed a significantly different expression level in rice leaves treated with chitosan compared to the control. Co-expression network analysis revealed nine of these proteins had significant coexpression with other genes from the three main biochemical network systems of photosynthesis, carbohydrate metabolism and cell redox homeostasis. More than 90 % of the genes positively co-expressed with these nine chitosanresponsive proteins were localized in chloroplasts, suggesting that chitosan enhanced the plant growth of rice seedlings via multiple and complex networks between the nucleus and chloroplast.

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The role of hydrogen peroxide in chitosan-induced resistance to osmotic stress in rice (Oryza sativa L.)

Cited by 97 publications

References 48 publications

Osmotic stress decreases PIP aquaporin transcripts in barley roots but H2O2 is not involved in this process

Osmotic stress decreases PIP aquaporin transcripts in barley roots but H2O2 is not involved in this process

Biostimulant activity of chitosan in horticulture

Chitosan enhances rice seedling growth via gene expression network between nucleus and chloroplast

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