The role of<i>Caenorhabditis elegans</i>in the discovery of natural products for healthy aging

Kirchweger, Benjamin; Zwirchmayr, Julia; Grienke, Ulrike; Rollinger, Judith M.

doi:10.1039/d3np00021d

Cited by 9 publications

(8 citation statements)

References 356 publications

(526 reference statements)

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“…Furthermore, results of the heat stress are sensitive to temperature fluctuations and thus require accurate temperature control of the incubator, along with proper handling 33 . Considering the importance of stress in the ageing process and its related diseases, stress resistance has evolved as an essential indicator for healthy ageing 14 , 24 that has accordingly been incorporated into the elaborated workflow.…”

Section: Resultsmentioning

confidence: 99%

“…C. elegans screenings are outstanding for their feasibility, versatile applicability, time-saving opportunities, and ethical compliance 12 , 13 . Its short life expectancy enables the detection of age-related changes and direct effects on life span within 2 – 3 weeks 14 , which is remarkably short compared to the use of other organisms, constituting a large advantage in ageing studies 15 . Even simple model organisms such as fruit flies or zebrafish can live for 3 and 60 months, respectively 15 .…”

Section: Introductionmentioning

confidence: 99%

“…Due to several similarities in processes like autophagy, apoptosis, fat storage, and neuronal regeneration, it is not surprising that C. elegans has become a key organism in drug discovery 18 , 19 , 20 . Thus, applying the roundworm as a model organism allows for the disclosure of pathways and targets, crucial for the biological effect 14 . In natural products research, C. elegans- based screenings are a convenient and straightforward method to identify health span-promoting compounds, and thus to bridge the gap between in vitro assays and in vivo trials.…”

Section: Introductionmentioning

confidence: 99%

“…A large number of natural compounds from almost all structural classes as well as multicomponent mixtures have already been investigated using the simple roundworm. When deciphering the bioactive constituents of an extract, C. elegans assays are valuable tools in the process of bioactivity-guided fractionation 14 . The miniaturized 96-well plate format enables the bioactivity assessment of various fractions of an extract in a time- and sample-saving manner 21 .…”

Section: Introductionmentioning

confidence: 99%

“…The miniaturized 96-well plate format enables the bioactivity assessment of various fractions of an extract in a time- and sample-saving manner 21 . The variety of assays using both wild-type animals (N2) and mutants seems to be almost unlimited, ranging from phenotypic assessment of life span, age, and disease-specific changes to detailed experiments focusing on various transcription factors 14 .…”

Section: Introductionmentioning

confidence: 99%

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Application of 3Rs in Caenorhabditis elegans Research for the Identification of Health-Promoting Natural Products

Redl,

Shayegan,

Rollinger

2024

Planta Med

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The average age of the population is increasing worldwide, which has a profound impact on our society. This leads to an increasing demand for medicines and requires the development of new strategies to promote health during the additional years. In the search for resources and therapeutics for improved health during an extended life span, attention has to be paid to environmental exposure and ecosystem burdens that inevitably emerge with the extended consumption of medicines and drug development, even in the preclinical stage. The hereby introduced sustainable strategy for drug discovery is built on 3Rs, “Robustness, Reliability, and saving Resources”, inspired by both the 3Rs used in animal experiments and environmental protection, and centers on the usefulness and the variety of the small model organism Caenorhabditis elegans for detecting health-promoting natural products. A workflow encompassing a multilevel screening approach is presented to maximize the amount of information on health-promoting samples, while considering the 3Rs. A detailed, methodology- and praxis-oriented compilation and discussion of proposed C. elegans health span assays and more disease-specific assays are presented to offer guidance for scientists intending to work with C. elegans, thus facilitating the initial steps towards the integration of C. elegans assays in their laboratories.

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Section: Resultsmentioning

confidence: 99%