The role of Parvimonas micra in intestinal tumorigenesis in germ-free and conventional APCmin/+ mice.

Yu, Jun; Zhao, Liuyang; Zhao, Risheng; Long, Xiaohang; Coker, Olabisi Oluwabukola; Sung, Joseph J.Y.

doi:10.1200/jco.2019.37.4_suppl.531

Cited by 15 publications

(15 citation statements)

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“…It has also been reported that P. micra may be involved in gut bacterial translocation and the upregulation of interleukins in the tumor microenvironment (55). A previous study demonstrated that APC Min/+ mice gavaged with P. micra exhibited a significantly higher tumor burden and tumor load, and cell proliferation was significantly higher in the colon tissues of P. micra gavaged germ-free mice compared with control mice (56). Furthermore, the tumor promoting effect of P. micra has been reported to be associated with altered immune responses and increased inflammation in the gut (50,56).…”

Section: Discussionmentioning

confidence: 93%

Alteration of the abundance of Parvimonas micra in the gut along the adenoma‑carcinoma sequence

Yang²,

Wang³

et al. 2020

Oncol Lett

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Parvimonas micra (P. micra) is reported to be associated with colorectal cancer (CRC). However, its association with colorectal adenoma (CRA) and its role in the initiation of colorectal tumors remain unknown. The present study aimed to clarify the relationship between P. micra and CRA and CRC by exploring the changes of P. micra abundance in an adenoma-carcinoma sequence in a new cohort and 4 public sequencing datasets. To investigate the alterations of P. micra abundance in the gut along the adenoma-carcinoma sequence, quantitative PCR (qPCR) was conducted to measure the relative abundance of P. micra in fecal samples from 277 subjects (128 patients with CRA, 66 patients with CRC and 83 healthy individuals, as controls) who underwent colonoscopy as outpatients. Then, the relative abundance of P. micra was analyzed in fecal samples from 596 subjects (185 healthy controls, 158 CRC, 253 CRA) in four public 16S rRNA sequencing datasets. The qPCR results demonstrated that the CRA group had an abundance of P. micra (P=0.2) similar to that of the healthy control group, while the CRC group had a significantly increased abundance (P=8.2x10-11). The level of P. micra effectively discriminated patients with CRC from healthy controls, while it poorly discriminated patients with CRA from healthy controls; with an area under the receiver operating characteristic curve of 0.867 for patients with CRC and 0.554 for patients with CRA. The same pattern of the alteration of P. micra abundance, which was low in healthy controls and patients with CRA but elevated in patients with CRC, was found in all four public sequencing datasets. These results suggested that P. micra was closely associated with, and may serve as a diagnostic marker for, CRC but not CRA. Moreover, it was indicated that P. micra may be an opportunistic pathogen of CRC, which may promote CRC development but serve a limited role in tumorigenesis.

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Section: Discussionmentioning

confidence: 93%

Alteration of the abundance of Parvimonas micra in the gut along the adenoma‑carcinoma sequence

Yang²,

Wang³

et al. 2020

Oncol Lett

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“…Mice treated with azoxymethane had a higher incidence of intestinal dysplasia following exposure to P. anaerobius . APC Min/+ mice gavaged with P. micra exhibited higher tumor burden and tumor load . However, their carcinogenic mechanisms have not been fully elucidated.…”

Section: Identification Of Colorectal Cancer‐associated Microbiota Bamentioning

confidence: 99%

“…25 APC Min/+ mice gavaged with P. micra exhibited higher tumor burden and tumor load. 26 However, their carcinogenic mechanisms have not been fully elucidated. In contrast to gastric cancer, which is caused mostly by a single species, Helicobacter pylori, CRC can be affected by various gut bacteria and their coexistence networks.…”

Section: Identific Ation Of Colorec Tal C An Cer-a Sso Ciated Mi Crmentioning

confidence: 99%

“…Bacteria related to colitis-associated CRC Enterotoxigenic Bacteroides fragilis 8 Adherent-invasive Escherichia coli 9 Genotoxic compound producing bacteria Pks + Escherichia coli strains (colibactin) 13 Clostridium hylemonae (deoxycholic acid) 12 Clostridium hiranonis (deoxycholic acid) 12 Bacteria related to non-colitis-associated CRC Fusobacterium nucleatum 10,11,[14][15][16]19,20 Parvimonas micra 20,26 Peptostreptococcus stomatis 19,20 Peptostreptococcus anaerobius 20,25 Atopobium parvulum 28 Actinomyces odontolyticus 28,30 factors in carcinogenesis, or whether they are a result of microbial adaptation to the tumor environment. Nevertheless, CRC-associated microbiota are currently globally accepted as CRC screening biomarkers that can be used as an alternative for the fecal occult blood test.…”

Section: Ta B L E 1 Association Between Gut Bacteria and Colorectal Cmentioning

confidence: 99%

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Significance of the gut microbiome in multistep colorectal carcinogenesis

2020

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Colorectal cancer (CRC) is highly prevalent worldwide. In 2018, there were over 1.8 million new cases. Most sporadic CRC develop from polypoid adenomas and are preceded by intramucosal carcinoma (stage 0), which can progress into more malignant forms. This developmental process is known as the adenoma‐carcinoma sequence. Early detection and endoscopic removal are crucial for CRC management. Accumulating evidence suggests that the gut microbiota is associated with CRC development in humans. Comprehensive characterization of this microbiota is of great importance to assess its potential as a diagnostic marker in the very early stages of CRC. In this review, we summarized recent studies on CRC‐associated bacteria and their carcinogenic mechanisms in animal models, human cell lines and human cohorts. High‐throughput technologies have facilitated the identification of CRC‐associated bacteria in human samples. We have presented our metagenome and metabolome studies on fecal samples collected from a large Japanese cohort that revealed stage‐specific phenotypes of the microbiota in CRC. Furthermore, we have discussed the potential carcinogenic mechanisms of the gut microbiota, from which we can infer whether changes in the gut microbiota are a cause or effect in the multi‐step process of CRC carcinogenesis.

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“…It is a producer of collagenase and of limited elastolytic and hemolytic activity [Ota-Tsuzuki and Alves Mayer 2010]. In a mouse CRC model, P. micra elicited increased Th2 and Th17 cells, decreased Th1 cells and increased inflammation [Yu et al 2019].…”

Section: Cohort Specific Speciesmentioning

confidence: 99%

The Presence of Periodontal Pathogens in Gastric Cancer

Leeuw¹,

Duval²

2020

Preprint

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BackgroundThe microbiome is thought to play a role in the development of gastric cancer (GC). Several studies have put forward putatively carcinogenic species in addition to Helicobacter pylori, but are not in perfect alignment, possibly due to variable parameters in the experiments, including downstream processing. Meta-analyses have not been published so far, so there is lack in clinical guidance beyond H. pylori eradication therapy.MethodsHere, we analysed gastric mucosa samples from nine public data sets, including GC samples. Using both unsupervised and supervised learning, we defined fine grain bacterial networks of gastric mucosa and identified species associated with tumor status of samples.ResultsWe found anatomic locations and cohort regions among the possible factors leading to the observation of study specific gastric microbiomes. Despite this variability, the periodontal species Fusobacterium nucleatum, Parvimonas micra and Peptostreptococcus stomatis were found in association with tumor status in several datasets. The three species were predicted to be in interaction by ecological network analysis and also formed the intersection of tumor associated species between four GC data sets and five colorectal cancer (CRC) data sets we reanalyzed. We formulated a probiotic composition putatively competing with the GC pathogen spectrum, from correlation analysis in a large superset of gut samples (n=17,800) from clinical- and crowd-sourced studies.ImplicationsThe overlapping bacterial pathogen spectrum between two gastrointestinal tumor types, GC and CRC, has implications for etiology, treatment and prevention. In vitro testing results reported in literature suggest H. pylori eradication treatment should be efficient against the GC pathogen spectrum, yet the existence of an upstream periodontal reservoir is of concern. To address this, we propose longer term use of the formulated probiotics composition.

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The role of Parvimonas micra in intestinal tumorigenesis in germ-free and conventional APC^min/+ mice.

Cited by 15 publications

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Alteration of the abundance of <em>Parvimonas micra</em> in the gut along the adenoma‑carcinoma sequence

Alteration of the abundance of <em>Parvimonas micra</em> in the gut along the adenoma‑carcinoma sequence

Significance of the gut microbiome in multistep colorectal carcinogenesis

The Presence of Periodontal Pathogens in Gastric Cancer

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The role of Parvimonas micra in intestinal tumorigenesis in germ-free and conventional APCmin/+ mice.

Cited by 15 publications

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Alteration of the abundance of <em>Parvimonas&nbsp;micra</em> in the gut along the adenoma‑carcinoma sequence

Alteration of the abundance of <em>Parvimonas&nbsp;micra</em> in the gut along the adenoma‑carcinoma sequence

Significance of the gut microbiome in multistep colorectal carcinogenesis

The Presence of Periodontal Pathogens in Gastric Cancer

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Product

Resources

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The role of Parvimonas micra in intestinal tumorigenesis in germ-free and conventional APC^min/+ mice.

Alteration of the abundance of <em>Parvimonas micra</em> in the gut along the adenoma‑carcinoma sequence

Alteration of the abundance of <em>Parvimonas micra</em> in the gut along the adenoma‑carcinoma sequence