The role of TFPI2 hypermethylation in the detection of gastric and colorectal cancer

Hu, Haochang; Chen, Xiaoying; Wang, Cheng; Jiang, Yuting; Li, Jingjing; Ying, Xiuru; Yang, Yong; Li, Bin; Zhou, Cong; Zhong, Jie; Wu, Dongping; Ying, Jieer; Duan, Shiwei

doi:10.18632/oncotarget.21097

Cited by 33 publications

(23 citation statements)

References 49 publications

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“…As the bridge between the genetic and environmental aspects, epigenetic modification is proved to play an important role in carcinogenesis 7,8 . DNA methylation, the most studied epigenetic regulatory mechanism, could behave as a powerful biomarker for early detection of lung cancer 9,10 .…”

Section: Introductionmentioning

confidence: 99%

Clinicopathological Significance and Diagnostic Value of DLEC1 Hypermethylation in Lung Cancer: A Meta-analysis

Mao

Guo

et al. 2019

J Nippon Med Sch

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Background: DLEC1 is a tumor-suppressor gene which plays a role in carcinogenesis. The purpose of the current study was to help establish the diagnostic performance of DLEC1 methylation in lung cancer. Methods: PubMed, Embase, CNKI, and Wanfang databases were searched to obtain eligible studies. The pooled odds ratios (ORs) and 95% confidence intervals (CIs) were calculated to estimate the strength of the associations. The diagnostic value was assessed by the summary receiver operating characteristics test. Results: A total of 7 articles, with 8 studies that included 673 lung cancer and 581 control samples, were collected in this meta-analysis. Our results showed a significant association of DLEC1 hypermethylation with lung cancer (P < 0.00001, OR = 13.93, 95% CI = 9.44-20.55). The frequency of DLEC1 methylation was significantly higher in squamous cell carcinoma (SCC) than adenocarcinoma (AC). Moreover, DLEC1 was more frequently methylated in patients with lung cancer aged 60 years or over, patients with lymphatic metastasis, or patients with stage III/IV lung cancer. In addition, there was a sensitivity value of 0.90 (95% CI = 0.86-0.93) and a specificity value of 0.60 (95% CI = 0.56-0.63), a pooled positivelikelihood ratio (PLR) of 2.27 (95% CI = 2.08-2.48), a pooled negative-likelihood ratio (NLR) of 0.17 (95% CI = 0.12-0.23), a diagnostic odds ratio (DOR) of 14.72 (10.09-21) and an area under the curve (AUC) of 0.8146 using DLEC1 methylation in the prediction of lung cancer risk. Conclusion: This meta-analysis confirms that DLEC1 methylation is a promising biomarker for lung cancer.

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Section: Introductionmentioning

confidence: 99%

Clinicopathological Significance and Diagnostic Value of DLEC1 Hypermethylation in Lung Cancer: A Meta-analysis

Mao

Guo

et al. 2019

J Nippon Med Sch

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“…qMSP was performed as described in our previous studies (20)(21)(22). The percent of methylated reference (PMR) was used to represent gene methylation (23,24). The genomic position and function annotations of EED were obtained from the university of California Santa Cruz genome browser (GRCh37/hg19; http://genome.ucsc.edu/index.…”

Section: Methodsmentioning

confidence: 99%

“…The empty pGL3 basic vector was used as the negative control and the pGL3 promoter vector (both Promega Cooperation, Madison, WI, uSA) was used as the positive control, which contained an SV40 promoter upstream of the luciferase gene. The plasmid transfection and the detection of luciferase activity were performed as previously described (23,27).…”

Section: Methodsmentioning

confidence: 99%

Significant association of EED promoter hypomethylation with colorectal cancer

et al. 2019

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Colorectal cancer (CRC) is one of the most common and serious types of malignancy worldwide. The embryonic ectoderm development (EED) gene is important to maintain transcriptional repressive states of genes over successive cell generations. The present study aimed to investigate the association between EED methylation and CRC. A total of 111 CRC tissue samples, 111 paired para-tumor tissues and 20 colorectal normal tissues were obtained for EED methylation assay, which was performed using a quantitative methylation-specific polymerase chain reaction. The percentage of methylated reference was calculated to represent the DNA methylation level. A dual-luciferase reporter gene assay was used to detect the gene promoter activity of a EED fragment. The current results revealed a significant difference in the EED methylation levels among tumor, para-tumor and normal colorectal tissues (tumor vs. para-tumor vs. normal, 5.03±4.61 vs. 8.65±11.50 vs. 40.12±45.31; F=45.014; P<0.0001). The dual-luciferase reporter gene assay demonstrated that the transcriptional activity of recombinant pGL3-EED plasmid was significantly higher compared with that of the pGL3-Basic control vector (fold-change, 3.15; P=0.014), which suggests the EED fragment can promote gene expression. In conclusion, the present study demonstrated that EED hypomethylation may be an important factor associated with CRC.

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“…The details of genomic DNA extraction and bisulfite conversion were as described in our previous study (20). The details of the qMSP and the validation of the qMSP products were the same as previously described (21)(22)(23)(24). The primer sequences for the six apoptosis-associated genes [TGFB1, BCL2 associated X, apoptosis regulator (BAX), IGFBP3, PRKC1, PSEN2 and CCL2] are presented in Table II.…”

Section: Subjectsmentioning

confidence: 99%

Association between the methylation of six apoptosis‑associated genes with autism spectrum disorder

Zhao

Zhou

et al. 2018

Mol Med Report

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Excessive apoptosis hinders the process of brain maturation and is regarded as one of the principal risk factors for the development of autism spectrum disorder (ASD). The aim of the present study was to investigate the association between the methylation of six apoptosis‑associated genes [transforming growth factor β 1 (TGFB1), BCL2 associated X, apoptosis regulator, insulin like growth factor binding protein 3, protein kinase C β 1, presenilin 2 and C‑C motif chemokine ligand 2] and ASD. Using quantitative methylation‑specific polymerase chain reaction technology, DNA methylation levels were detected in 42 autistic and 26 control subjects. The logistic regression analysis results demonstrated that of the six genes, only TGFB1 was significantly hypomethylated in peripheral blood samples from children with autism compared with control samples (mean percentage of methylated reference, 0.011% vs. 0.019%; age‑adjusted P=0.028). In addition, TGFB1 methylation was identified to be positively associated with the interaction ability score from the Autism Behavior Checklist (r=0.452; P=0.035). These data suggested that decreased TGFB1 methylation may contribute to the development of ASD.

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The role of TFPI2 hypermethylation in the detection of gastric and colorectal cancer

Cited by 33 publications

References 49 publications

Clinicopathological Significance and Diagnostic Value of <i>DLEC1</i> Hypermethylation in Lung Cancer: A Meta-analysis

Clinicopathological Significance and Diagnostic Value of <i>DLEC1</i> Hypermethylation in Lung Cancer: A Meta-analysis

Significant association of EED promoter hypomethylation with colorectal cancer

Association between the methylation of six apoptosis‑associated genes with autism spectrum disorder

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