The role of IL-10 in regulating inflammation and gut microbiome in mice consuming milk kefir and orally challenged with <i>S</i>. Typhimurium

Pereira, Mariana de Fátima Albuquerque; Ávila, Larissa Gabriela; Cruz, Bruna Cristina dos Santos; Silva, Bruno Campos; Oliveira, Leandro Licursi de; Gonçalves, Reggiani Vilela; Ribon, Andréa de Oliveira Barros; Mendes, Tiago; Pelúzio, Maria do Carmo Gouveia

doi:10.1039/d2fo04063h

Cited by 6 publications

(3 citation statements)

References 46 publications

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“…They are signaling molecules in the immune system that regulate and modulate the occurrence and intensity of immune responses. IL-1β, IL-6, and TNF-α are important pro-inflammatory factors in the body ( Song et al, 2022 ), while IL-10 is an important anti-inflammatory and immune-regulating cytokine ( Albuquerque Pereira et al, 2023 ), playing an important role in alleviating immune damage caused by infections. IL-2and IL-4 are involved in regulating immune response reactions, playing important roles in host cellular and humoral immunity ( Ullrich et al, 2021 ; Kung et al, 2022 ), respectively; IFN-γ plays a key role in defending against intracellular bacterial infections ( Kak et al, 2018 ).…”

Section: Discussionmentioning

confidence: 99%

“…TNF-α plays a role in apoptosis, inflammation, and the immune response to cancer and infection. IFN-γ is critical for innate and adaptive immunity against viral and intracellular bacterial infections and for tumor control ( Kak et al, 2018 ; Ullrich et al, 2021 ; Behzadi et al, 2022 ; Kung et al, 2022 ; Song et al, 2022 ; Albuquerque Pereira et al, 2023 ; Mukherjee et al, 2023 ). Moreover, studies show that attenuated live vaccines of Salmonella are low in virulence to the host and can induce a strong and lasting mucosal and humoral immune response ( Tennant and Levine, 2015 ), effectively reducing bacterial adhesion and colonization.…”

Section: Introductionmentioning

confidence: 99%

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The peptidoglycan-associated lipoprotein gene mutant elicits robust immunological defense in mice against Salmonella enteritidis

Zhao,

Duan,

Zhang

et al. 2024

Front. Microbiol.

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BackgroundSalmonella enteritidis (S. enteritidis), a zoonotic pathogen with a broad host range, presents a substantial threat to global public health safety. Vaccination stands as an effective strategy for the prevention and control of S. enteritidis infection, highlighting an immediate clinical need for the creation of safe and efficient attenuated live vaccines.MethodsIn this study, a S. enteritidis peptidoglycan-associated lipoprotein (pal) gene deletion strain (Δpal), was constructed. To assess its virulence, we conducted experiments on biofilm formation capability, motility, as well as cell and mouse infection. Subsequently, we evaluated the immune-protective effect of Δpal.ResultsIt was discovered that deletion of the pal gene reduced the biofilm formation capability and motility of S. enteritidis. Cell infection experiments revealed that the Δpal strain exhibited significantly decreased abilities in invasion, adhesion, and intracellular survival, with downregulation of virulence gene expression, including mgtC, invH, spvB, sipA, sipB, ssaV, csgA, and pipB. Mouse infection experiments showed that the LD50 of Δpal increased by 104 times, and its colonization ability in mouse tissue organs was significantly reduced. The results indicated that the pal gene severely affected the virulence of S. enteritidis. Further, immunogenicity evaluation of Δpal showed a significant enhancement in the lymphocyte transformation proliferation capability of immunized mice, producing high titers of specific IgG and IgA, suggesting that Δpal possesses good immunogenicity. Challenge protection tests demonstrated that the strain could provide 100% immune protection against wild-type strains in mice.DiscussionThis study proves that the pal gene influences the virulence of S. enteritidis, and Δpal could serve as a candidate strain for attenuated live vaccines, laying the foundation for the development of attenuated live vaccines against Salmonella.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The peptidoglycan-associated lipoprotein gene mutant elicits robust immunological defense in mice against Salmonella enteritidis

Zhao,

Duan,

Zhang

et al. 2024

Front. Microbiol.

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“…Other inflammatory cytokines, IL-1β, IL-1RA, Il-10, IL-12p70, IL-13, TNF-a, and IFN-γ not only changed in levels after burn injury but also were trended downward when the burn severity increased in the remodeling phase. IL-10 and IFN-γ, as anti-inflammatory cytokines, were reported to promote regenerative healing and prevent scar formation by in vivo and in vitro experiments [ 34 , 35 ].…”

Section: Discussionmentioning

confidence: 99%

Characteristics of Serum Exosomes after Burn Injury and Dermal Fibroblast Regulation by Exosomes In Vitro

Ding,

Pan,

Raj

et al. 2023

Cells

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(1) Background: Exosomes (EXOs) have been considered a new target thought to be involved in and treat wound healing. More research is needed to fully understand EXO characteristics and the mechanisms of EXO-mediated wound healing, especially wound healing after burn injury. (2) Methods: All EXOs were isolated from 85 serum samples of 29 burn patients and 13 healthy individuals. We characterized the EXOs for morphology and density, serum concentration, protein level, marker expression, size distribution, and cytokine content. After a confirmation of EXO uptake by dermal fibroblasts, we also explored the functional regulation of primary human normal skin and hypertrophic scar fibroblast cell lines by the EXOs in vitro, including cell proliferation and apoptosis. (3) Results: EXOs dynamically changed their morphology, density, size, and cytokine level during wound healing in burn patients, which were correlated with burn severity and the stages of wound healing. EXOs both from burn patients and healthy individuals stimulated dermal fibroblast proliferation and apoptosis. (4) Conclusions: EXO features may be important signals that influence wound healing after burn injury; however, to understand the mechanisms by which EXOs regulates the fibroblasts in healing wounds, further studies will be required.

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Effects of kefir consumption on gut microbiota and health outcomes in women with polycystic ovary syndrome

Çıtar Dazıroğlu,

Acar Tek,

Cevher Akdulum

et al. 2024

Food Science & Nutrition

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Polycystic Ovary Syndrome (PCOS), which is common among women of reproductive age, is characterized by low‐grade chronic inflammation and is associated with several health problems and dysbiosis. Kefir has been shown to have many beneficial health effects; however, its effect on PCOS is unknown. This study aimed to examine the effect of kefir on the intestinal microbiota and health outcomes in PCOS. In this intervention study, 17 women with PCOS consumed 250 mL/day of kefir (containing Lactobacillus kefiranofaciens subsp. kefiranofaciens, Lactobacillus kefiranofaciens subsp. kefirgranum, Lactobacillus kefiri, Lactobacillus acidophilus, Lactobacillus parakefiri, Lactobacillus bulgaricus, Lactobacillus reuteri, Lactobacillus casei, Lactobacillus fermentum, Lactobacillus helveticus, Lactococcus lactis, Leuconostoc mesentereoides, Bifidobacterium bifidum, Streptococcus thermophilus, Kluyveromyces marxianus, Kluyveromyces lactis, Acetobacter pasteurianus, and Saccharomyces cerevisiae) for 8 weeks. Food consumption and physical activity records, anthropometrical measurements, quality of life, and fecal and blood samples were taken at the study's beginning and end. Quality of life in mental health (58.8 ± 15.08; 64.0 ± 15.23, respectively) and physical function (95.00 and 100.00, respectively) categories showed a significant increase after kefir intervention (p < .05). Additionally, Interleukin‐6 (IL‐6), one of the inflammatory cytokines, significantly decreased (174.00 and 109.10 ng/L, respectively) (p < .05). The intestinal barrier permeability was evaluated with zonulin, and no significant change was observed. Gut microbiota analysis showed that while the relative abundance of the class Bacilli and genus Lactococcus significantly increased, the genus Holdemania decreased with kefir consumption (p < .05). In conclusion, kefir appears to be beneficial for improving the microbiota and some health outcomes, like reducing inflammation and improving quality of life in PCOS. Therefore, kefir may be useful in the treatment of PCOS.

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The role of IL-10 in regulating inflammation and gut microbiome in mice consuming milk kefir and orally challenged with S. Typhimurium

Abstract: Kefir has been suggested as possible bacterial prophylaxis against Salmonella and IL-10 production seems to be crucial in the mice pathogenesis disease. This study aimed to evaluate the role of...

Cited by 6 publications

References 46 publications

The peptidoglycan-associated lipoprotein gene mutant elicits robust immunological defense in mice against Salmonella enteritidis

The peptidoglycan-associated lipoprotein gene mutant elicits robust immunological defense in mice against Salmonella enteritidis

Characteristics of Serum Exosomes after Burn Injury and Dermal Fibroblast Regulation by Exosomes In Vitro

Effects of kefir consumption on gut microbiota and health outcomes in women with polycystic ovary syndrome

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