The Role of Immune Microenvironment in Maxillofacial Bone Homeostasis

Li, Na; Fu, Lin; Li, Zehan; Ke, Yanxiong; Wang, Yanqiu; Wu, Jintao; Yu, Jinhua

doi:10.3389/fdmed.2021.780973

Cited by 2 publications

(2 citation statements)

References 127 publications

(127 reference statements)

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“…Cytokines are important in regulating bone homeostasis and immune functions ( Horowitz, 1998 ). The differentiation and activation of the bone cells including the osteoclasts and osteoblasts are driven by cytokines and immune cells ( Li et al, 2021 ). Thus, the role of various cytokines on bone metabolism during LPS-induced immune challenge was investigated.…”

Section: Discussionmentioning

confidence: 99%

“…In addition, it was found that the mRNA expression of TNF-α , IL-17, and IFN-γ tended to increase in the tibia of LCA-fed hens challenged with LPS injection. In a recent review, these immune cytokines (TNF-α, IL-17, and IFN-γ) were identified as particularly significant in stimulating bone resorption and suppressing bone formation, which ultimately leads to inflammatory bone loss ( Li et al, 2021 ). Thus, it is evident that low Ca supply during immune challenge would exacerbate the incidence of bone loss and dysregulate bone homeostasis in aged laying hens.…”

Section: Discussionmentioning

confidence: 99%

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Effects of low dietary calcium and lipopolysaccharide challenges on production performance, eggshell quality, and bone metabolism of laying hens

Li,

Uyanga,

Jiao

et al. 2024

Front. Physiol.

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Dietary calcium supply is essential for bone development and egg production in laying hens. This study investigated the effects of low dietary calcium and lipopolysaccharide (LPS) induced immune challenge in aged laying hens. A total of thirty-two Hy-Line Brown laying hens at 80 weeks old with an average laying rate of 62% were randomly divided into two groups and fed a normal calcium diet (3.57% Ca, NCA) or low calcium diet (2.08% Ca, LCA). At 88 weeks, the experiment was designed using a 2 × 2 factorial arrangement, and hens were intraperitoneally injected with saline (SAL) or LPS (0.5 mg/kg, 0.5 mg/kg, or 1.5 mg/kg body weight) once every 48 h intervals over 5 days. Production performance, egg quality, and bone physiology were evaluated. Results showed that LPS challenge decreased the hen-day egg production, egg mass, and eggshell traits (p < 0.05), but increased (p < 0.05) the calcium content of the tibia compared to SAL-injected hens. LCA diet decreased (p < 0.05) the hen-day egg production, and eggshell traits such as weight, percentage, strength, and thickness compared to the NCA diet. LCA diet increased the serum alkaline phosphatase (ALP) activity (p < 0.01) and tibial expression of ALP (p < 0.05) compared to NCA diet. LPS injection suppressed both the serum ALP activity (p < 0.05) and tibial expression of ALP (p < 0.001) compared to SAL injection. Furthermore, LPS injection increased (p < 0.05) the expression of both pro and anti-inflammatory cytokines in the spleen and tibia. The expression of cathepsin K (Cts K) and matrix metalloproteinase 9 (MMP-9) were downregulated by LPS injection (p < 0.001). Broken and shell-less egg production and calcium content of eggshell, as well as tibial mRNA expression of osteocalcin (Ocn), tumor necrosis factor-alpha (TNF-α) and tartrate-resistant acid phosphatase (TRAP) were affected by the interaction (p < 0.05) of diet and injection. Therefore, this study demonstrated that to certain extents, low dietary calcium and LPS challenge dysregulated bone homeostasis and metabolism, with detrimental effects on the performance and eggshell quality of aged laying hens.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Effects of low dietary calcium and lipopolysaccharide challenges on production performance, eggshell quality, and bone metabolism of laying hens

Li,

Uyanga,

Jiao

et al. 2024

Front. Physiol.

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Expression features of T-lymphocytes, B-lymphocytes and macrophages in the post-traumatic regenerate of the mandible rats under conditions of filling a bone defect with hydroxyapatite-containing osteotropic material and thymalin injecting the surrounding soft tissues

Boiko,

Malanchuk,

Myroshnychenko

et al. 2024

Polski Merkuriusz Lekarski

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Aim: The purpose of the study was to determine the features of the expression of T-lymphocytes, B-lymphocytes, macrophages in the post-traumatic regenerate of the mandible rats under conditions of filling a bone defect with hydroxyapatite-containing osteotropic material and thymalin injecting the surrounding soft tissues. Materials and Methods: An experiment was conducted on 48 mature rats of the WAG population weighing 160-180 grams. Four groups were formed. Group 1 included 12 rats with a simulated holey defect in the lower jaw. Group 2 included 12 rats with a simulated holey defect in the lower jaw followed by its closure with hydroxyapatite-containing osteotropic material (bone graft “Biomin GT”). Group 3 included 12 rats with a simulated holey defect in the lower jaw with injecting the surrounding soft tissues with thymalin. Group 4 included 12 rats with a simulated holey defect in the lower jaw followed by its closure with hydroxyapatite-containing osteotropic material (bone graft “Biomin GT”) and injecting the surrounding soft tissues with thymalin. The material for the morphological study was a fragment of the lower jaw from the area of the simulated holey defect. An immunohistochemical study was performed using monoclonal antibodies to CD68, CD20, CD163, CD86, CD3. Results: A comprehensive experimental and morphological study conducted by the authors revealed that thymalin injection of the soft tissues surrounding the bone defect of the lower jaw, filled with hydroxyapatite-containing osteotropic material “Biomin GT”, stimulates local immune reactions in the post-traumatic regenerate, which is manifested, firstly, by an increase in the number T-lymphocytes on the 3rd day of the experiment and their increase up to the 28th day; secondly, by increasing the number of B-lymphocytes on the 14th day of the experiment with their further increase up to the 28th day; thirdly, by increasing the number of macrophages on the 3rd day of the experiment and their growth up to the 28th day; fourth, changes in macrophages phenotypes (decrease in the number of M1-macrophages and increase in the number of M2-macrophages). Conclusions: Stimulation of local immune reactions in the post-traumatic regenerate can be one of the mechanisms that activate reparative osteogenesis in the lower jaw of rats under the conditions of filling bone defects with hydroxyapatite-containing osteotropic material “Biomin GT” and thymalin injecting the surrounding soft tissues.

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The Role of Immune Microenvironment in Maxillofacial Bone Homeostasis

Cited by 2 publications

References 127 publications

Effects of low dietary calcium and lipopolysaccharide challenges on production performance, eggshell quality, and bone metabolism of laying hens

Effects of low dietary calcium and lipopolysaccharide challenges on production performance, eggshell quality, and bone metabolism of laying hens

Expression features of T-lymphocytes, B-lymphocytes and macrophages in the post-traumatic regenerate of the mandible rats under conditions of filling a bone defect with hydroxyapatite-containing osteotropic material and thymalin injecting the surrounding soft tissues

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