The Role of Interleukin-10 in Systemic Inflammatory Response Syndrome with Sepsis

Kawai, Shinya; Sakayori, Susumu; Watanabe, Hidehiro; Nakagawa, Tomoyuki; Inoue, Gen; Kobayashi, Hiroyoshi

doi:10.1007/bf02491513

Cited by 8 publications

(3 citation statements)

References 21 publications

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“…[ 41 ]. IL-10 pretreatment could prevent the TNF response to LPS in mice [ 42 ]. In a human study, the IL-10/lymphocyte ratio was significantly higher in non-surviving patients than in surviving patients, indicating sepsis-induced IL-10-related immunosuppression [ 43 ].…”

Section: Discussionmentioning

confidence: 99%

Ex Vivo Evaluation of the Sepsis Triple Therapy High-Dose Vitamin C in Combination with Vitamin B1 and Hydrocortisone in a Human Peripheral Blood Mononuclear Cells (PBMCs) Model

et al. 2021

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Sepsis is an extremely complex clinical syndrome, usually involving an excessive inflammatory response including an overshooting cytokine release that damages tissue and organs of the patient. Due to the severity of this condition, it is estimated that over 11 million people die from sepsis each year. Despite intensive research in the field, there is still no specific therapy for sepsis. Many sepsis patients show a marked deficiency of vitamin C. 9 out of 10 sepsis patients have a hypovitaminosis C, and every third patient even shows a clinical deficiency in the scurvy range. In addition, low vitamin C levels of intensive care sepsis patients correlate with a higher need for vasopressors, higher Sequential Organ Failure Assessment (SOFA) scores, and increased mortality. Based on this observation and the conducted clinical trials using vitamin C as sepsis therapy in intensive care patients, the aim of the present ex vivo study was to evaluate the effects of high-dose vitamin C alone and in a triple combination supplemented with vitamin B1 (thiamine) and hydrocortisone on the lipopolysaccharide (LPS)-induced cytokine response in peripheral blood mononuclear cells (PBMCs) from healthy human donors. We found that all corticosteroid combinations strongly reduced the cytokine response on RNA- and protein levels, while high-dose vitamin C alone significantly diminished the PBMC mediated secretion of the cytokines interleukin (IL)-10, IL-23, and monocyte chemo-attractant protein (MCP-1), which mediate the inflammatory response. However, vitamin C showed no enhancing effect on the secretion of further cytokines studied. This data provides important insights into the possible immunomodulatory function of vitamin C in an ex vivo setting of human PBMCs and the modulation of their cytokine profile in the context of sepsis. Since vitamin C is a vital micronutrient, the restoration of physiologically adequate concentrations should be integrated into routine sepsis therapy, and the therapeutic effects of supraphysiological concentrations of vitamin C in sepsis patients should be further investigated in clinical trials.

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Section: Discussionmentioning

confidence: 99%

Ex Vivo Evaluation of the Sepsis Triple Therapy High-Dose Vitamin C in Combination with Vitamin B1 and Hydrocortisone in a Human Peripheral Blood Mononuclear Cells (PBMCs) Model

et al. 2021

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“…20,21 It was observed that, with the ICR mouse, inflammatory changes in the lung were extremely rare, while in the nude mouse, strong inflammatory change was seen. It was also observed that a reduction in lung tissue injury was apparent in the cohort with the simultaneous administration of LPS and IL-10.…”

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confidence: 99%

Effect of interleukin-10 (IL-10) on experimental LPS-induced acute lung injury

Inoue

2000

Journal of Infection and Chemotherapy

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“…It would suggest that both anti-inflammatory cytokines for IL-5 and IL-10 play a role in various kidney diseases by activating an anti-inflammatory response, immunoregulation, and relieving kidney tissue fibrosis. IL-10 has also been shown to cause immunosuppression associated with various forms of trauma by attenuating TNF-α and IL-1β while enhancing IFN-γ production in plasma and urine [ 101 ]. These cytokines are released into the blood or urine and may serve as biomarkers of early AKI.…”

Section: Discussionmentioning

confidence: 99%

Acute phase reactions in Daboia siamensis venom and fraction-induced acute kidney injury: the role of oxidative stress and inflammatory pathways in in vivo rabbit and ex vivo rabbit kidney models

Chaiyabutr,

Noiprom,

Promruangreang

et al. 2024

J. Venom. Anim. Toxins incl. Trop. Dis

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Background: This study examines the direct nephrotoxic effects of Daboia siamensis venom (RVV) and venom fractions in in vivo and isolated perfused kidneys (IPK) to understand the role of inflammation pathways and susceptibility to oxidative stress in venom or fraction-induced acute renal failure. Methods: We administered RVV and its venom fractions (PLA 2 , MP, LAAO, and PDE) to rabbits in vivo and in the IPK model. We measured oxidative stress biomarkers (SOD, CAT, GSH, and MDA) in kidney tissue, as well as inflammatory cytokines (TNF-α, IL-1β, IFN-γ, IL-4, IL-5, and IL-10), MDA and GSH levels in plasma and urine. We also calculated fractional excretion (FE) for pro-/anti-inflammatory cytokines and oxidative stress biomarkers, including the ratios of pro-/anti-inflammatory cytokines in urine after envenomation. Results: In both kidney models, significant increases in MDA, SOD, CAT, and GSH levels were observed in kidney tissues, along with elevated concentrations of MDA and GSH in plasma and urine after injecting RVV and venom fractions. Moreover, RVV injections led to progressive increases in FE MDA and decreases in FE GSH. The concentrations of IL-4, IL-5, IL-10, IFN-γ, and TNF-α in plasma increased in vivo , as well as in the urine of the IPK model, but not for IL-1β in both plasma and urine after RVV administrations. Urinary fractional excretion of TNF-α, IL-1β, IFN-γ, IL-4, IL-5, and IL-10 tended to decrease in vivo but showed elevated levels in the IPK model. A single RVV injection in vivo disrupted the balance of urinary cytokines, significantly reducing either the TNF-α/IL-10 ratio or the IFN-γ/IL-10 ratio. Conclusion: RVV induces renal tubular toxicity by increasing oxidative stress production and elevating inflammatory cytokines in urine. During the acute phase of acute kidney injury, the balance of urine cytokines shifts toward anti-inflammatory dominance within the first two hours post-RVV and venom fractions.

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The Role of Interleukin-10 in Systemic Inflammatory Response Syndrome with Sepsis

Cited by 8 publications

References 21 publications

Ex Vivo Evaluation of the Sepsis Triple Therapy High-Dose Vitamin C in Combination with Vitamin B1 and Hydrocortisone in a Human Peripheral Blood Mononuclear Cells (PBMCs) Model

Ex Vivo Evaluation of the Sepsis Triple Therapy High-Dose Vitamin C in Combination with Vitamin B1 and Hydrocortisone in a Human Peripheral Blood Mononuclear Cells (PBMCs) Model

Effect of interleukin-10 (IL-10) on experimental LPS-induced acute lung injury

Acute phase reactions in Daboia siamensis venom and fraction-induced acute kidney injury: the role of oxidative stress and inflammatory pathways in in vivo rabbit and ex vivo rabbit kidney models

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