The role of intestinal dendritic cells subsets in the establishment of food allergy

Abstract

Sensitization to PE in this mouse model was accompanied by a shift in DC subsets in intestinal tissues towards more CD11b(+) DC and less CD103(+) DC. No significant changes in the plasmacytoid DC (pDC) numbers were observed. Flt3L treatment, resulting in the expansion of all DC subtypes, inhibited allergic manifestations in our model, including Th2 cytokine production, PE-specific IgE and PE-induced mast cell degranulation. pDC depletion reversed Flt3L-induced inhibition of IgE responses and mast cell degranulation. conclusions and clinical relevance: The establishment of food allergy is accompanied by profound changes in DC subsets in the intestine towards more inflammatory CD11b(+) DC. In addition, expansion of DC numbers by Flt3L, in particular pDC, inhibits the establishment of allergic manifestations in the intestine. These findings are of relevance for understanding the role of DC subsets early during the process of allergic sensitization, and may lead to new therapeutic or prophylactic opportunities to prevent food allergy.

The Mucosal Factors Retinoic Acid and TGF-β1 Induce Phenotypically and Functionally Distinct Dendritic Cell Types

“…Increased numbers of CD11b+ DCs and decreased numbers of CD103+ DCs were observed after sensitization with peanut allergens in a mouse peanut allergy model [14]. Tolerogenic CD103+ DCs are known for their different migratory capacity (homing to mesenteric lymph node; MLN) and capacity to induce peripheral tolerance by inducing FoxP3+ CD4+ Tregs [1,5,15].…”

Mechanisms of Allergic Sensitization to Foods: Bypassing Immune Tolerance Pathways

“…Therefore, OX40L on this DC subset Allergic Sensitization to Foods seemed to be selectively responsible for the allergic sensitization component of the adjuvant activity of CT. 40 Within the lamina propria it has been found that allergic sensitization is associated with an expansion of CD11b 1 DCs and a relative reduction of CD103 1 DCs. 41 These data indicate that the phenotype of the gastrointestinal DC determines the outcome of sensitization versus tolerance to foods.…”

Intestinal Microbiocenosis, Food Tolerance and Food Allergy. Current State of a Problem

“…Одновременное введение токсинов бактери-ального происхождения с антигенами влияет на экспрес-сию рецепторов главного комплекса гистосовместимо-сти (MHC II) и костимуляторных молекул на дендритных клетках и индуцирует T х2 -ответ с повышением продукции ИЛ 4 и интенсификацией синтеза антигенспецифиче-ских IgE и IgG 2a [50,51]. В экспериментальных исследо-ваниях показано, что воздействие холерных токсинов индуцирует аллергическую сенсибилизацию к экстракту арахиса, вызывая сдвиг подвидов дендритных клеток в собственной пластинке и пейеровых бляшках с более провоспалительных CD11b(+) на менее толерогенные CD103(+) клетки [52]. Более того, обнаружена повышен-ная экспрессия Т-клеточной (иммуноглобулин и муцин) доменовой молекулы (TIM-4) в дендритных клетках кост-ного мозга мышей после конкурентного воздействия холерных токсинов и аллергенов арахиса по сравнению с таковыми, обработанными только одними аллергенами [53].…”