The role of intestinal immune cells and matrix metalloproteinases in inflammatory bowel disease

Abstract

Inflammatory bowel disease (IBD) has become globally intractable. MMPs play a key role in many inflammatory diseases. However, little is known about the role of MMPs in IBD. In this study, IBD expression profiles were screened from public Gene Expression Omnibus datasets. Functional enrichment analysis revealed that IBD-related specific functions were associated with immune pathways. Five MMPS-related disease markers, namely MMP-9, CD160, PTGDS, SLC26A8, and TLR5, were selected by machine learning and the correlation between each marker and immune cells was evaluated. We then induced colitis in C57 mice using sodium dextran sulfate and validated model construction through HE staining of the mouse colon. WB and immunofluorescence experiments confirmed that the expression levels of MMP-9, PTGDS, SLC26A8, and CD160 in colitis were significantly increased, whereas that of TLR5 were decreased. Flow cytometry analysis revealed that MMPs regulate intestinal inflammation and immunity mainly through CD8 in colitis. Our findings reveal that MMPs play a crucial role in the pathogenesis of IBD and are related to the infiltration of immune cells, suggesting that MMPs may promote the development of IBD by activating immune infiltration and the immune response. This study provides insights for further studies on the occurrence and development of IBD.

Kumquat pomace removal of free polyphenol alleviates induced acute enteritis and restores gut microbiota in dextran sodium sulphate‐treated mice

“…KNEP eased acute enteritis in DSS‐induced IBD mice in a dose‐dependent manner (Lang‐Schwarz et al., 2021). We also found that the colon tissue of mice with acute enteritis was devoid of mucosa, the crypts contained several neutrophils, the structure was incomplete, and the inflammatory cell infiltration was severe (Mei et al., 2023). KNEP improved anti‐inflammatory activity in colon, inhibited the expression of inflammatory factors, promoted repair of the damage to the intestinal flora structure caused by IBD, and improved and protected the intestinal structure stimulated by DSS in mice.…”

Qingre Jiangni formula improves esophageal epithelial barrier function in gastroesophageal reflux rats through male hormones and the p38/MAPK pathway

“…GERD occurs when stomach contents flow back into the esophagus due to attenuated esophageal clearance and antireflux barrier mechanisms. The resulting mucosal injury is a result of both direct damage from gastric juice and an immune and inflammatory response, wherein inflammatory cytokines released by the esophageal mucosal epithelium induce neutrophil migration, leading to inflammation [23]. The onset and progression of GERD are multifaceted.…”

Metalloproteinases between History, Health, Disease, and the Complex Dimension of Social Determinants of Health

“…MMPs are involved in several types of diseases and generally related to three main mechanisms: tissue destruction, such as cancer (e.g., acute myeloid leukemia and bladder, brain, breast, colorectal, endometrial, gastric, head and neck, liver, lung, ovarian, pancreas, prostate, renal, skin, and thyroid cancer), diabetes, inflammatory diseases (e.g., psoriasis, osteoarthritis, rheumatoid arthritis, inflammatory bowel disease), chronic wounds (e.g., pressure ulcers, vascular ulcers), periodontal diseases, hypertension, kidney diseases (e.g., chronic kidney disease, glomerular disease), myocardial infarction, and neurogenerative disease (e.g., multiple sclerosis, Parkinson's disease, Alzheimer's disease, amyotrophic lateral sclerosis); fibrosis, such as liver cirrhosis, otosclerosis, gynecological disorders (e.g., polycystic ovarian syndrome, spontaneous abortion, preeclampsia), and atherosclerosis-related diseases; and weakening of the ECM, such as chronic venous disease, pulmonary embolism, aneurysms, and dilated cardiomyopathy. Often, more than one mechanism overlaps in determining the disease [2,3,7,11,19,22,[37][38][39][40][41][42][43][44][45][46][47][48][49][50].…”