The role of intracellular β-glucosidase in cellulolytic response induction in filamentous fungus Penicillium verruculosum

Kislitsin, Valeriy Yu; Chulkin, Andrey M.; Dotsenko, Anna S.; Sinelnikov, Igor G.; Sinitsyn, Arkady P.; Rozhkova, Aleksandra M.

doi:10.1016/j.resmic.2023.104178

Cited by 1 publication

(1 citation statement)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As in bacteria, catabolite repression in fungi is a common mechanism of gene expression regulation that prevents the synthesis of enzymes and secondary metabolites unnecessary for the cell under certain conditions. CCR is exerted on many genes encoding glycoside hydrolases such as fungal β-1,3-glucanase and β-1,6-glucanase [36], intracellular β-glucosidase in P. verruculosum [37], or the penicillin-producing enzyme cluster in P. chrysogenum [38].…”

Section: Discussionmentioning

confidence: 99%

Glucose Catabolite Repression Participates in the Regulation of Sialidase Biosynthesis by Antarctic Strain Penicillium griseofulvum P29

Abrashev,

Krumova,

Petrova

et al. 2024

JoF

View full text Add to dashboard Cite

Sialidases (neuraminidases) catalyze the removal of terminal sialic acid residues from glycoproteins. Novel enzymes from non-clinical isolates are of increasing interest regarding their application in the food and pharmaceutical industry. The present study aimed to evaluate the participation of carbon catabolite repression (CCR) in the regulation of cold-active sialidase biosynthesis by the psychrotolerant fungal strain Penicillium griseofulvum P29, isolated from Antarctica. The presence of glucose inhibited sialidase activity in growing and non-growing fungal mycelia in a dose- and time-dependent manner. The same response was demonstrated with maltose and sucrose. The replacement of glucose with glucose-6-phosphate also exerted CCR. The addition of cAMP resulted in the partial de-repression of sialidase synthesis. The CCR in the psychrotolerant strain P. griseofulvum P29 did not depend on temperature. Sialidase might be subject to glucose repression by both at 10 and 25 °C. The fluorescent assay using 4MU-Neu5Ac for enzyme activity determination under increasing glucose concentrations evidenced that CCR may have a regulatory role in sialidase production. The real-time RT-PCR experiments revealed that the sialidase gene was subject to glucose repression. To our knowledge, this is the first report that has studied the effect of CCR on cold-active sialidase, produced by an Antarctic strain.

show abstract

Section: Discussionmentioning

confidence: 99%