The role of L-type amino acid transporters in the uptake of glyphosate across mammalian epithelial tissues

Xu, Jiaqiang; Li, Gao; Wang, Zhuoyi; Si, Luqin; He, Sijie; Cai, Jialing; Huang, Jiangeng; Donovan, Maureen D.

doi:10.1016/j.chemosphere.2015.11.062

Cited by 38 publications

(21 citation statements)

References 40 publications

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“…Furthermore, the deletion of the gltT gene also confers resistance to the herbicide glufosinate resembling the structure of glutamate. Recently, it has been reported that amino acid transporters are involved in the transport of glyphosate across mammalian epithelial tissues (Xu et al, 2016). GltP is the major glutamate transporter in E. coli during aerobic growth (Schellenberg and Furlong, 1977).…”

Section: Discussionmentioning

confidence: 99%

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Identification of the first glyphosate transporter by genomic adaptation

Wicke

Schulz

Lentes

et al. 2019

Environmental Microbiology

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Summary The soil bacterium Bacillus subtilis can get into contact with growth‐inhibiting substances, which may be of anthropogenic origin. Glyphosate is such a substance serving as a nonselective herbicide. Glyphosate specifically inhibits the 5‐enolpyruvyl‐shikimate‐3‐phosphate (EPSP) synthase, which generates an essential precursor for de novo synthesis of aromatic amino acids in plants, fungi, bacteria and archaea. Inhibition of the EPSP synthase by glyphosate results in depletion of the cellular levels of aromatic amino acids unless the environment provides them. Here, we have assessed the potential of B. subtilis to adapt to glyphosate at the genome level. In contrast to Escherichia coli, which evolves glyphosate resistance by elevating the production and decreasing the glyphosate sensitivity of the EPSP synthase, B. subtilis primarily inactivates the gltT gene encoding the high‐affinity glutamate/aspartate symporter GltT. Further adaptation of the gltT mutants to glyphosate led to the inactivation of the gltP gene encoding the glutamate transporter GltP. Metabolome analyses confirmed that GltT is the major entryway of glyphosate into B. subtilis. GltP, the GltT homologue of E. coli also transports glyphosate into B. subtilis. Finally, we found that GltT is involved in uptake of the herbicide glufosinate, which inhibits the glutamine synthetase.

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Section: Discussionmentioning

confidence: 99%

“…Thus, GltP could also be involved in glyphosate uptake in E. coli. Recently, it has been reported that amino acid transporters are involved in the transport of glyphosate across mammalian epithelial tissues (Xu et al, 2016). Thus, in prokaryotic as well as in eukaryotic cells glyphosate may enter the cell via amino acid transporters.…”

Section: Discussionmentioning

confidence: 99%

Identification of the first glyphosate transporter by genomic adaptation

Wicke

Schulz

Lentes

et al. 2019

Environmental Microbiology

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“…One possible glyphosate‐resistance mechanism is the non‐recognition of the active uptake carrier system, because the inhibition of glyphosate‐loading into the phloem was observed in some glyphosate‐resistant weeds . Furthermore, a recent study shows that L‐type amino acid transporters (LAT1/LAT2) play major roles in the uptake of glyphosate across mammalian epithelial tissues . Phosphite, the active metabolite of fosetyl‐Al, is a highly ambimobile fungicide .…”

Section: Carrier‐mediated Transport Of Agrochemicals In Plantsmentioning

confidence: 99%

“…31 Furthermore, a recent study shows that L-type amino acid transporters (LAT1/LAT2) play major roles in the uptake of glyphosate across mammalian epithelial tissues. 32 Phosphite, the active metabolite of fosetyl-Al, is a highly ambimobile fungicide. 33 This agrochemical which can also be used as a fertilizer and weedicide is translocated by both high-and low-affinity phosphate transporters.…”

Section: Carrier-mediated Transport Of Agrochemicals In Plantsmentioning

confidence: 99%

Vectorizing agrochemicals: enhancing bioavailability via carrier‐mediated transport

Marivingt-Mounir

et al. 2019

Pest Management Science

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Systemicity of agrochemicals is an advantageous property for controlling phloem sucking insects, as well as pathogens and pests not accessible to contact products. After the penetration of the cuticle, the plasma membrane constitutes the main barrier to the entry of an agrochemical into the sap flow. The current strategy for developing systemic agrochemicals is to optimize the physicochemical properties of the molecules so that they can cross the plasma membrane by simple diffusion or ion trapping mechanisms. The main problem with current systemic compounds is that they move everywhere within the plant, and this non‐controlled mobility results in the contamination of the plant parts consumed by vertebrates and pollinators. To achieve the site‐targeted distribution of agrochemicals, a carrier‐mediated propesticide strategy is proposed in this review. After conjugating a non‐systemic agrochemical with a nutrient (α‐amino acids or sugars), the resulting conjugate may be actively transported across the plasma membrane by nutrient‐specific carriers. By applying this strategy, non‐systemic active ingredients are expected to be delivered into the target organs of young plants, thus avoiding or minimizing subsequent undesirable redistribution. The development of this innovative strategy presents many challenges, but opens up a wide range of exciting possibilities. © 2018 Society of Chemical Industry

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“…Glyphosate has recently been shown to be taken up by nasal and gastrointestinal mucosal cells via active transport across L-type amino acid channels, LAT1/ LAT2 [51]. This finding is not altogether unexpected, since glyphosate is an amino acid.…”

Section: 1mentioning

confidence: 92%