The role of larval fat cells in adult<i>Drosophila melanogaster</i>

Aguila, Jerell R; Suszko, Justin; Gibbs, Allen G.; Hoshizaki, Deborah K.

doi:10.1242/jeb.001586

Cited by 186 publications

(168 citation statements)

References 32 publications

Supporting

Mentioning

154

Contrasting

Unclassified

Order By: Relevance

“…The larval and the adult fat body are composed of two different cell lineages. The larval fat body represents a contiguous organ of a fairly constant number of postmitotic, large endoreplicative cells, which undergo histolysis shortly after the adult fly ecloses (Aguila et al, 2013(Aguila et al, , 2007. In contrast, the adult fat body is believed to be composed of diploid cells derived from cell clusters in the larval body wall and from adepithelial cells of the imaginal discs (Hoshizaki et al, 1995).…”

Section: The Fat Body and Oenocytesmentioning

confidence: 99%

Drosophilaas a model to study obesity and metabolic disease

Musselman

Kühnlein

2018

Journal of Experimental Biology

181

130

View full text Add to dashboard Cite

Excess adipose fat accumulation, or obesity, is a growing problem worldwide in terms of both the rate of incidence and the severity of obesity-associated metabolic disease. Adipose tissue evolved in animals as a specialized dynamic lipid storage depot: adipose cells synthesize fat (a process called lipogenesis) when energy is plentiful and mobilize stored fat (a process called lipolysis) when energy is needed. When a disruption of lipid homeostasis favors increased fat synthesis and storage with little turnover owing to genetic predisposition, overnutrition or sedentary living, complications such as diabetes and cardiovascular disease are more likely to arise. The vinegar fly Drosophila melanogaster (Diptera: Drosophilidae) is used as a model to better understand the mechanisms governing fat metabolism and distribution. Flies offer a wealth of paradigms with which to study the regulation and physiological effects of fat accumulation. Obese flies accumulate triacylglycerols in the fat body, an organ similar to mammalian adipose tissue, which specializes in lipid storage and catabolism. Discoveries in Drosophila have ranged from endocrine hormones that control obesity to subcellular mechanisms that regulate lipogenesis and lipolysis, many of which are evolutionarily conserved. Furthermore, obese flies exhibit pathophysiological complications, including hyperglycemia, reduced longevity and cardiovascular functionsimilar to those observed in obese humans. Here, we review some of the salient features of the fly that enable researchers to study the contributions of feeding, absorption, distribution and the metabolism of lipids to systemic physiology.

show abstract

Section: The Fat Body and Oenocytesmentioning

confidence: 99%

Drosophilaas a model to study obesity and metabolic disease

Musselman

Kühnlein

2018

Journal of Experimental Biology

181

130

View full text Add to dashboard Cite

show abstract

“…The Drosophila larval fat body is the equivalent of the mammalian liver and adipose tissue, functioning to store nutrients and generate energy. During metamorphosis, the fat body is extensively remodelled dissociating from a single layer of cells to become individual cells which are then eliminated during early adulthood (Aguila et al, 2007, Nelliot et al, 2006. As well as undergoing autophagy in response to starvation, the Drosophila fat body undergoes ecdysone-mediated autophagy during the transition from larval to pupal development via downregulation of the PI3K pathway (Lindmo et al, 2006, Liu et al, 2013, Rusten et al, 2004, Scott et al, 2004.…”

Section: Fat Body and Neuronal Remodellingmentioning

confidence: 99%

Ecdysone-mediated programmed cell death in Drosophila

Nicolson

Denton²,

Kumar³

2015

Int. J. Dev. Biol.

View full text Add to dashboard Cite

During Drosophila development, the steroid hormone ecdysone plays a key role in the transition from embryo into larva and then into pupa. It is during larval-pupal metamorphosis that extensive programmed cell death occurs to remove large obsolete larval tissues. During this transition, ecdysone pulses control the expression of specific transcription factors which drive the expression of key genes involved in cell death, thus spatially and temporally controlling programmed cell death. Ecdysone also controls cell death in specific larval and adult tissues. This review focuses on the current knowledge of ecdysone-mediated cell death in Drosophila.

show abstract

“…A striking exception to the loss of larval tissues is the fat body, which undergoes tissue remodeling into individual cells during the pre-pupal stages of metamorphosis (Nelliot et al, 2006). These cells survive metamorphosis (Aguila et al, 2007) and are present in the newly eclosed adult. The larval fat cells presumably support the D. melanogaster pupae, which do not feed, and the newly eclosed adult, which remains inactive for approximately 8h until the wings expand and the cuticle tans (Chiang, 1963;Edgecomb et al, 1994).…”

Section: Introductionmentioning

confidence: 99%

“…Surprisingly, newly eclosed adult females are three times more starvation resistant than mature females (3 or 10days old). This difference is not age dependent per se, because retention of the larval fat cells by inhibition of PCD increases the starvation resistance of older adults (Aguila et al, 2007). Thus, larval fat cells represent an important and accessible energy reservoir in the adult female, yet once these reserves are released (and presumably taken up by adult tissues) they are not available to maintain the adult during starvation.…”

Section: Introductionmentioning

confidence: 99%

“…Briefly, ectopic expression of the cell death inhibitor gene, Drosophila inhibitor of apoptosis 1 (diap1), in the larval fat body was achieved using the GAL4/UAS system (Brand and Perrimon, 1993). Construction of flies with the genotype y w; ) is described by Aguila et al (Aguila et al, 2007). A stock carrying the UAS-diap1 transgene, P{w[+mC]={UAS-DIAP1.H}1 (abbreviated UAS-diap), was obtained from the Bloomington Stock Center (Bloomington, IN, USA) and crossed to Lsp2-GAL4 to drive ectopic expression of diap1 specifically in the larval fat cells, thereby inhibiting PCD in these cells (Aguila et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Contribution of larval nutrition to adult reproduction inDrosophila melanogaster

Aguila

Hoshizaki

Gibbs

2012

Journal of Experimental Biology

Self Cite

View full text Add to dashboard Cite

SUMMARYWithin the complex life cycle of holometabolous insects, nutritional resources acquired during larval feeding are utilized by the pupa and the adult. The broad features of the transfer of larval resources to the pupae and the allocation of larval resources in the adult have been described by studies measuring and tracking macronutrients at different developmental stages. However, the mechanisms of resource transfer from the larva and the factors regulating the allocation of these resources in the adult between growth, reproduction and somatic maintenance are unknown. Drosophila melanogaster presents a tractable system in which to test cellular and tissue mechanisms of resource acquisition and allocation because of the detailed understanding of D. melanogaster development and the experimental tools to manipulate its tissues across developmental stages. In previous work, we demonstrated that the fat body of D. melanogaster larvae is important for survival of starvation stress in the young adult, and suggested that programmed cell death of the larval fat cells in the adult is important for allocation of resources for female reproduction. Here, we describe the temporal uptake of larval-derived carbon by the ovaries, and demonstrate the importance of larval fat-cell death in the maturation of the ovary and in fecundity. Larvae and adults were fed stable carbon isotopes to follow the acquisition of larval-derived carbon by the adult ovaries. We determined that over half of the nutrients acquired by the ovaries in 2-day-old adult females are dependent upon the death of the fat cells. Furthermore, when programmed cell death is inhibited in the larval fat cells, ovarian development was depressed and fecundity was reduced.

show abstract

The role of larval fat cells in adultDrosophila melanogaster

Cited by 186 publications

References 32 publications

Drosophilaas a model to study obesity and metabolic disease

Drosophilaas a model to study obesity and metabolic disease

Ecdysone-mediated programmed cell death in Drosophila

Contribution of larval nutrition to adult reproduction inDrosophila melanogaster

Contact Info

Product

Resources

About