The Role of Lectins in Finfish: A Review

Elumalai, Preetham; Rubeena, Abdul Salam; Arockiaraj, Jesu; Wongpanya, Ratree; Cammarata, Matteo; Ringø, Einar; Vaseeharan, Baskaralingam

doi:10.1080/23308249.2018.1520191

Cited by 46 publications

(27 citation statements)

References 142 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Fish lectins are reported to possess antimicrobial effects. In the presence of Ca 2+ , C-type lectins initiate a broad range of biological processes such as adhesion, endocytosis, and pathogen neutralization ( 102 ). The skin mucus of fish fed 25IM diet uniquely expressed superoxide dismutase, which is an antioxidant enzyme ( 103 , 104 ) and a marker of stress response ( 32 ).…”

Section: Discussionmentioning

confidence: 99%

Dietary Inclusion of Black Soldier Fly (Hermetia Illucens) Larvae Meal and Paste Improved Gut Health but Had Minor Effects on Skin Mucus Proteome and Immune Response in Atlantic Salmon (Salmo Salar)

et al. 2021

View full text Add to dashboard Cite

The present study investigated effects of dietary inclusion of black soldier fly larvae (BSFL) (Hermetia illucens) meal and paste on gut health, plasma biochemical parameters, immune response and skin mucus proteome in pre-smolt Atlantic salmon (Salmo salar). The seven-week experiment consisted of seven experimental diets: a control diet based on fishmeal and plant protein (Control-1); three BSFL meal diets, substituting 6.25% (6.25IM), 12.5% (12.5IM) and 25% (25IM) of protein; two BSFL paste diets, substituting 3.7% (3.7IP) and 6.7% (6.7IP) of protein and an extra control diet with 0.88% of formic acid (Control-2). The 6.25IM diet reduced enterocyte steatosis in pyloric caeca, improved distal intestine histology, and reduced IgM in distal intestine. The fish fed 12.5IM diet reduced enterocyte steatosis in pyloric caeca, improved distal intestine histology, had a higher plasma lysozyme content compared to 6.25IM, and tend to increase phagocytic activity in head-kidney macrophages-like cells. On the other hand, 25IM diet improved distal intestine histology, but showed mild-moderate enterocyte steatosis in pyloric caeca, increased IFNγ and reduced IgM in distal intestine. In the case of BSFL paste diets, 3.7IP diet caused mild inflammatory changes in distal intestine, although it reduced enterocyte steatosis in pyloric caeca. The 6.7IP diet reduced enterocyte steatosis in pyloric caeca and improved distal intestine histology. Increasing level of BSFL meal in the diet linearly decreased plasma C-reactive protein, whereas increasing level of BSFL paste linearly increased plasma antioxidant capacity. Dietary inclusion of BSFL meal and paste had minor effects on the expression profile of proteins in skin mucus and no effects on immune markers in splenocytes. BSFL meal showed no negative effect on liver and muscle health as indicated by plasma alanine aminotranseferase, asparate aminotransferase and creatine kinase. The present study showed that replacing conventional protein sources with low to moderate levels of BSFL meal (6.25% and 12.5%) or paste (3.7% and 6.7%) reduced enterocyte steatosis in pyloric caeca, while replacing up to 25% with BSFL meal or 6.7% with paste improved distal intestine histology. Further, dietary inclusion of BSFL meal and paste had minor effects on skin mucus proteome and immune response in Atlantic salmon.

show abstract

Section: Discussionmentioning

confidence: 99%

Dietary Inclusion of Black Soldier Fly (Hermetia Illucens) Larvae Meal and Paste Improved Gut Health but Had Minor Effects on Skin Mucus Proteome and Immune Response in Atlantic Salmon (Salmo Salar)

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Fundamentally, the glycosylated membrane is a biomimetic membrane with glycocalyx-like surface. , As is well-known, glycocalyx plays extremely important roles in living cells. Carbohydrate–protein interactions are fundamental scientific issues in many cellular processes involving normal physiology as well as disease. , In particular, carbohydrate–microbial lectin interaction is the critical recognition factor for bacterial infection and bacterial adhesion. , Thus, unsurprisingly, the glycosylated membranes not only have excellent surface hydrophilicity and a biocompatible microenvironment, but they have specific recognition to target biomacromolecules, including proteins and microbe species. , And this has largely led to enhanced microbes adhering and biofilm formation due to the unique chemo–biological properties of carbohydrates.…”

Section: Results and Discussionmentioning

confidence: 99%

Biofilm-Integrated Glycosylated Membrane for Biosuccinic Acid Production

Gao

Wang

et al. 2021

ACS Appl. Bio Mater.

View full text Add to dashboard Cite

Biofilm-based cell-immobilized fermentation technology is regarded as the technique with the most potential for biobased product (chemicals, biofuelss materials, etc.) production in industry. Glycosylated membrane can mimic natural extracellular matrix (ECM) and improve cell adhesion and biofilm formation based on carbohydrate−microbial lectin interaction. Here, we applied glycosylated membrane with rhamnose modified surface for constructing Actinobacillus succinogenes biofilm and producing biosuccinic acid. Polymer hollow fiber (PHF) membrane surface was first modified by glycosylation based on physical adsorption approach. The approach is simple, green, and suitable for scaleamplification. Then, the microbial biofilm formed dramatically on the modified membrane surface. And for subsequent biosuccinic acid production, the maximum titer of succinic acid reached 67.3 g/L, and the yield was 0.82 g/g. Compared with free cell fermentation, the titer and yield increased by 18% and 9% in this biofilm-based cell-immobilized fermentation system, respectively. Importantly, the production efficiency of biosuccinic acid increased obviously for subsequent biofilm-based cell-immobilized fermentation. In addition, the biofilm-integrated glycosylated membrane showed high reusability for succinic acid production. This result is important for developing biofilms for a wide range of applications in bioproduct (chemicals, biofuels, materials, etc.) production.

show abstract

“…Lectins were identified as part of the barramundi immune response to foreign antigens (LPS) as early as 35 days posthatch (Xia and Yue, 2010). Lectins are assumed to mediate pathogen recognition, cell adhesion, the activation of complement pathway, and facilitate pathogen clearance by phagocytosis, thus playing an important role in innate immunity and disease resistance in fish (Elumalai et al, 2019). While the phagosome was the most enriched immunerelated pathway in the livers of SDD barramundi, there was an overall downregulation of C3 and C1q, key complement proinflammatory genes in sick fish.…”

Section: Discussionmentioning

confidence: 99%

Scale Drop Disease Virus (SDDV) and Lates calcarifer Herpes Virus (LCHV) Coinfection Downregulate Immune-Relevant Pathways and Cause Splenic and Kidney Necrosis in Barramundi Under Commercial Farming Conditions

et al. 2021

View full text Add to dashboard Cite

Marine farming of barramundi (Lates calcarifer) in Southeast Asia is currently severely affected by viral diseases. To better understand the biological implications and gene expression response of barramundi in commercial farming conditions during a disease outbreak, the presence of pathogens, comparative RNAseq, and histopathology targeting multiple organs of clinically “sick” and “healthy” juveniles were investigated. Coinfection of scale drop disease virus (SDDV) and L. calcarifer herpes virus (LCHV) were detected in all sampled fish, with higher SDDV viral loads in sick than in healthy fish. Histopathology showed that livers in sick fish often had moderate to severe abnormal fat accumulation (hepatic lipidosis), whereas the predominant pathology in the kidneys shows moderate to severe inflammation and glomerular necrosis. The spleen was the most severely affected organ, with sick fish presenting severe multifocal and coalescing necrosis. Principal component analysis (PC1 and PC2) explained 70.3% of the observed variance and strongly associated the above histopathological findings with SDDV loads and with the sick phenotypes, supporting a primary diagnosis of the fish being impacted by scale drop disease (SDD). Extracted RNA from kidney and spleen of the sick fish were also severely degraded likely due to severe inflammation and tissue necrosis, indicating failure of these organs in advanced stages of SDD. RNAseq of sick vs. healthy barramundi identified 2,810 and 556 differentially expressed genes (DEGs) in the liver and muscle, respectively. Eleven significantly enriched pathways (e.g., phagosome, cytokine-cytokine-receptor interaction, ECM-receptor interaction, neuroactive ligand-receptor interaction, calcium signaling, MAPK, CAMs, etc.) and gene families (e.g., tool-like receptor, TNF, lectin, complement, interleukin, chemokine, MHC, B and T cells, CD molecules, etc.) relevant to homeostasis and innate and adaptive immunity were mostly downregulated in sick fish. These DEGs and pathways, also previously identified in L. calcarifer as general immune responses to other pathogens and environmental stressors, suggest a failure of the clinically sick fish to cope and overcome the systemic inflammatory responses and tissue degeneration caused by SDD.

show abstract

The Role of Lectins in Finfish: A Review

Cited by 46 publications

References 142 publications

Dietary Inclusion of Black Soldier Fly (Hermetia Illucens) Larvae Meal and Paste Improved Gut Health but Had Minor Effects on Skin Mucus Proteome and Immune Response in Atlantic Salmon (Salmo Salar)

Dietary Inclusion of Black Soldier Fly (Hermetia Illucens) Larvae Meal and Paste Improved Gut Health but Had Minor Effects on Skin Mucus Proteome and Immune Response in Atlantic Salmon (Salmo Salar)

Biofilm-Integrated Glycosylated Membrane for Biosuccinic Acid Production

Scale Drop Disease Virus (SDDV) and Lates calcarifer Herpes Virus (LCHV) Coinfection Downregulate Immune-Relevant Pathways and Cause Splenic and Kidney Necrosis in Barramundi Under Commercial Farming Conditions

Contact Info

Product

Resources

About