The Role of Leukocyte-Platelet-Rich Fibrin in Promoting Wound Healing in Diabetic Foot Ulcers

Abstract

To explore the effect of leukocyte-platelet-rich fibrin (L-PRF) on promoting wound healing in diabetic foot ulcers. A total of 42 patients with diabetic foot ulcers at our hospital from January 2017 to July 2020 were retrospectively analyzed. A control group and a PRF group were established. The two groups of patients underwent debridement. In the platelet-rich fibrin (PRF) group, autologous L-PRF was used to cover ulcer wounds. One time each week, Vaseline gauze was used to cover the ulcer wounds. In contrast, the control group was treated with the external application of mupirocin ointment and recombinant human epidermal growth factor gel (yeast). Two times each week, the sterile Vaseline gauze was covered with a bandage. Both groups were treated for 5 weeks. The wound recovery of the two groups was observed. During the early stage of treatment (first and second weeks) for diabetic foot ulcers, the wound healing rate was significantly better with L-PRF treatment than traditional treatment. For later-stage treatment (third to fifth weeks), the overall cure rate was higher with L-PRF than the traditional treatment method. L-PRF can effectively promote wound healing in diabetic foot ulcers.

Quantitative Association of Platelet-Rich Fibrin (PRF) and Hyperacute Serum (HAS) with Glycemic Control (HbA1c) in Chronic Periodontitis Patients: An Ex vivo Study

“…PRF is a safe, appropriate, easy-to-use adjunctive treatment modality with substantial possibility for the management of chronic skin wounds like pressure, diabetic foot, or venous-leg ulcers without any adversative effects. [ 27 , 28 ] However, studies have suggested that the reduced or compromised wound healing capacity in uncontrolled diabetic patients may be due to a reduction in growth factors and prolonged inflammatory responses due to increased proinflammatory cytokines in T2DM patients. [ 22 , 29 , 30 ] Thus, a modification in the protocol (rpm and time) for obtaining PRF in diabetic patients may be investigated.…”

Fibrinogen Concentrations in Liquid PRF Using Various Centrifugation Protocols

“…It is relevant, however, to determine how much fibrinogen is in each fraction and the yield of clottable components present to predict the ability of liquid PRF to generate PRF membranes that are presumably easier to handle than standard PRF where the time between vein puncture and centrifugation is a critical determinant of the size of solid PRF [ 24 ]. These liquid PRF-derived membranes may be prepared in larger quantities and even stored in a cooling environment until their use, which may be more ideal for the treatment of diabetic wounds [ 25 ]. PRF membranes can be customized in shape into larger-sized membranes by pooling of PPP and BC from various tubes.…”

Antimicrobial effect of platelet‐rich fibrin: A systematic review of in vitro evidence‐based studies

“…Recently, PRF has been used as an adjuvant in treating wounds (e.g., diabetic foot) to form a protective barrier to increase local cellular activity, causing tissue recovery. 3 This action is due to PRF's tissue regeneration potential. 4 PRF's involvement in wound healing may go beyond acting as a protective barrier.…”