The Role Of Limited Respiration In The Incomplete Oxidation Of Glucose By Saccharomyces Cerevisiae

Rieger, Martin; Käppeli, Othmar; Fiechter, Armin

doi:10.1099/00221287-129-3-653

Cited by 121 publications

(164 citation statements)

References 18 publications

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“…Both fermentative and oxidative metabolic pathways co-exist (De Deken 1966;Mazzoni et al 1992) and thus we propose to classify K. laais as a short-term Crabtree-negative and a long-term Crabtree-, or Warburg-positive yeast. De Deken (1966) has suggested that the Crabtree effect is related to, or mediated by, glucose repression, while other authors have suggested that the effect is attributable to a limited capacity of the respiratory chain (Rieger et al 1983; K/ippeli and Sonnleitner 1986). Neither view is likely to be correct.…”

Section: Discussionmentioning

confidence: 99%

Carbon catabolite regulation of transcription of nuclear genes coding for mitochondrial proteins in the yeast Kluyveromyces lactis

1995

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Disclaimer/Complaints regulationsIf you believe that digital publication of certain material infringes any of your rights or (privacy) interests, please let the Library know, stating your reasons. In case of a legitimate complaint, the Library will make the material inaccessible and/or remove it from the website. Please Ask the Library: http://uba.uva.nl/en/contact, or a letter to: Library of the University of Amsterdam, Secretariat, Singel 425, 1012 WP Amsterdam, The Netherlands. You will be contacted as soon as possible. Carbon catabolite regulation of transcription of nuclear genes coding for mitochondrial proteins in the yeast Kluyveromyces lactis Received: 7 February 1995 Abstract Promoter regions of the K1QCR7, KlQCR8 and K1CYC1 genes, coding for subunits of the bclcomplex and cytochrome c respectively, in the shortterm Crabtree-negative yeast Kluyveromyces Iactis differ markedly in sequence from their Saccharomyces cerevisiae counterparts. They have, however, conserved very similar configurations of binding-site motifs for various transcription factors known to be involved in global and carbon-source regulation in S. cerevisiae. To investigate the carbon source-dependent expression of these genes in K. lactis, we have carried out mediumshift experiments and determined transcript levels during the shifts. In sharp contrast to the situation in S. cerevisiae, the level of expression in K. lactis is not affected when glucose is added to a non-fermentable carbon-source medium. However, the genes are not constitutively expressed, but become significantly induced when the cells are shifted from glucose to a nonfermentable carbon source. Finally, induction of transcriptional activation does not occur in media containing both glucose and non-fermentable carbon sources.

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Section: Discussionmentioning

confidence: 99%

Carbon catabolite regulation of transcription of nuclear genes coding for mitochondrial proteins in the yeast Kluyveromyces lactis

1995

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“…This so-called 'short-term Crabtree-effect' has been ascribed to the inability of such yeasts to instantaneously enhance their respiration rate (Petrik et al 1983;Rieger et al 1983;Kfippeli 1986). This would leave them unable to completely respire the increased flux of sugar entering the cell (and the glycolytic pathway) as a consequence of the increased extracellular sugar concentration.…”

Section: Transient State Kinetics Of Sugar Metabolismmentioning

confidence: 99%

Kinetics of growth and sugar consumption in yeasts

Dijken¹,

Weusthuis²,

Pronk³

1993

Antonie van Leeuwenhoek

187

112

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An overview is presented of the steady-and transient state kinetics of growth and formation of metabolic byproducts in yeasts. Saccharomyces cerevisiae is strongly inclined to perform alcoholic fermentation. Even under fully aerobic conditions, ethanol is produced by this yeast when sugars are present in excess. This so-called 'Crabtree effect' probably results from a multiplicity of factors, including the mode of sugar transport and the regulation of enzyme activities involved in respiration and alcoholic fermentation. The Crabtree effect in S. cerevisiae is not caused by an intrinsic inability to adjust its respiratory activity to high glycolytic fluxes. Under certain cultivation conditions, for example during growth in the presence of weak organic acids, very high respiration rates can be achieved by this yeast. S. cerevisiae is an exceptional yeast since, in contrast to most other species that are able to perform alcoholic fermentation, it can grow under strictly anaerobic conditions.'Non-Saccharomyces' yeasts require a growth-limiting supply of oxygen (i.e. oxygen-limited growth conditions) to trigger alcoholic fermentation. However, complete absence of oxygen results in cessation of growth and therefore, ultimately, of alcoholic fermentation. Since it is very difficult to reproducibly achieve the right oxygen dosage in large-scale fermentations, non-Saccharornyces yeasts are therefore not suitable for largescale alcoholic fermentation of sugar-containing waste streams. In these yeasts, alcoholic fermentation is also dependent on the type of sugar. For example, the facultatively fermentative yeast Candida utilis does not ferment maltose, not even under oxygen-limited growth conditions, although this disaccharide supports rapid oxidative growth.

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“…The method developed by von Meyenburg (1969) involved the construction of gas balances for S. cerevisiae at a number of different dilution rates, both in the fully respiratory range of (Barford & Hall 1979;Rieger et al 1983). Furthermore, it is highly unlikely that YA'r• is constant at all growth rates, since it is well known that the protein content of S. cerevisiae increases significantly with increasing growth rate (Furukawa et al 1983;Verduyn et al 1990a;Fiechter & v o n Meyenburg 1969), both under anaerobic and aerobic conditions.…”

Section: Calculation Of P/o-ratios For Growth On Glucosementioning

confidence: 99%

A theoretical evaluation of growth yields of yeasts

Verduyn

Stouthamer

Scheffers

et al. 1991

Antonie van Leeuwenhoek

216

176

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Growth yields of Saccharomyces cerevisiae and Candida utilis in carbon-limited chemostat cultures were evaluated. The yields on ethanol and acetate were much lower in S. cerevisiae, in line with earlier reports that site I phosphorylation is absent in this yeast. However, during aerobic growth on glucose both organisms had the same cell yield. This can be attributed to two factors: -S. cerevisiae had a lower protein content than C. utilis; -uptake of glucose by C. utilis requires energy whereas in S. cerevisiae it occurs via facilitated diffusion. Theoretical calculations showed that, as a result of these two factors, the ATP requirement for biomass formation in C. utilis is 35% higher than in S. cerevisiae (theoretical YATP values of 20.8 and 28.1, respectively). The experimental YATP for anaerobic growth of S. cerevisiae on glucose was 16 g biomass-mol ATP -1.In vivo P/O-ratios can be calculated for aerobic growth on ethanol and acetate, provided that the gap between the theoretical and experimental ATP requirements as observed for growth on glucose is taken into account. This was done in two ways: -via the assumption that the gap is independent of the growth substrate (i.e. a fixed amount of ATP bridges the difference between the theoretical and experimental values). -alternatively, on the assumption that the difference is a fraction of the total ATP expenditure, that is dependent on the substrate. Calculations of P/O-ratios for growth of both yeasts on glucose, ethanol, and acetate made clear that only by assuming a fixed difference between theoretical and experimental ATP requirements, the P/O-ratios are more or less independent of the growth substrate. These P/O-ratios are approximately 30% lower than the calculated mechanistic values.

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The Role Of Limited Respiration In The Incomplete Oxidation Of Glucose By Saccharomyces Cerevisiae

Cited by 121 publications

References 18 publications

Carbon catabolite regulation of transcription of nuclear genes coding for mitochondrial proteins in the yeast Kluyveromyces lactis

Carbon catabolite regulation of transcription of nuclear genes coding for mitochondrial proteins in the yeast Kluyveromyces lactis

Kinetics of growth and sugar consumption in yeasts

A theoretical evaluation of growth yields of yeasts

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