The role of liposome–protamine–DNA nanoparticles containing CpG oligodeoxynucleotides in the course of infection induced by Leishmania major in BALB/c mice

Alavizadeh, Seyedeh Hoda; Badiee, Ali; Khamesipour, Ali; Jalali, Seyed Amir; Firouzmand, Hengameh; Abbasi, Azam; Jaafari, Mahmoud Reza

doi:10.1016/j.exppara.2012.06.014

Cited by 19 publications

(6 citation statements)

References 40 publications

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“…Authors claimed that the early IL-4 production did not hinder the Th1 response, which is crucial for the priming of long-term CD8 1 T-cell memory responses. The same cationic liposomes but complexed with cationic polymer (protamine) condensing CpG-ODN mixed with leishmania parasites induced a milder leishmania lesion and a minimum number of L. major in the spleen and lymph nodes, accompanied by a Th1 type of immune response with a preponderance of IgG2a isotype (Alavizadeh et al, 2012). These results suggested that immune modulation using NPs might be a practical approach to improve the safety of LZ.…”

Section: Nanomedical Prophylactic Strategiesmentioning

confidence: 80%

Nanomedical Therapeutic and Prophylaxis Strategies Against Intracellular Protozoa in the Americas

Morilla

Romero

2015

Nanotechnology in Diagnosis, Treatment and Prophylaxis of Infectious Diseases

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Section: Nanomedical Prophylactic Strategiesmentioning

confidence: 80%

Nanomedical Therapeutic and Prophylaxis Strategies Against Intracellular Protozoa in the Americas

Morilla

Romero

2015

Nanotechnology in Diagnosis, Treatment and Prophylaxis of Infectious Diseases

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“…OVA‐FITC protein was purchased from Invitrogen (Carlsbad, CA, USA). CpG‐ODN1826 (5‐TCCATGA CG TTCCTGA CG TT‐3) was purchased from Mycrosynth (Balgach, Switzerland) .…”

Section: Methodsmentioning

confidence: 99%

Enhanced immune response induced by P5 HER2/neu‐derived peptide‐pulsed dendritic cells as a preventive cancer vaccine

Gholizadeh

Tavakkol‐Afshari

Nikpoor

et al. 2017

J Cellular Molecular Medi

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Dendritic cells are special and powerful antigen‐presenting cells that can induce primary immune responses against tumour‐associated antigens. They can present antigens via both MHC‐I and MHC‐II, so they have the ability to stimulate both cytotoxic T lymphocytes and T helper cells. Furthermore, CD8+ cytotoxic T lymphocytes require activation by CD4+ T cells. This requires a CD4+T cell activator molecule, of which PADRE is one of the best. We chose an approach to use both of these important arms of the immune system. We prepared dendritic cells from mouse bone marrow, loaded them with our target peptides (P5 peptide alone or P5 + PADRE), and then injected these pulsed dendritic cells alone or in combination with CpG‐ODN (as adjuvant) into BALB/C mice. After the last boosting dose, mice were inoculated with TUBO cells, which overexpress HER2/neu. Two weeks after the tumour cell injection, immunological tests were performed on splenocyte suspensions, and the remaining mice were evaluated for tumour growth and survival. Our data indicate the formulation that contains PADRE plus P5 loaded onto DC in combination with CpG‐ODN was the most effective formulation at inducing immune responses. Interferon production in CD4+ and CD8+ gated cells, cytotoxicity rates of target cells and mice survival were all significantly greater in this group than in controls, and all the mice in this group were tumour‐free throughout the experiment. Based on our results and the role of HER2/neu as a candidate in human immunotherapy, this approach may be an effective cancer treatment.

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“…It has managed to invent nanoparticles, as carriers, for drug delivery; liposomal formulation of amphotericin B reduces its toxicity profile (Date et al, 2007). Nanotechnology has significantly enhanced the conventional leishmanization procedure by inducing a Th1-type immune response in BALB/ c during L. major infection using a liposome-protamine-DNA nanoparticle with immunostimulatory CpG (Alavizadeh et al, 2012), (Fakhraee et al, 2016). Nano-liposomal formulation of 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP) and soluble leishmanial antigen (SLA) causes protection against L. major infection and enhances Th1-type immune response.…”

Section: Nanotechnology As a Drug Delivery Systemmentioning

confidence: 99%

“…Immunomodulators Nicholls et al, 2010, Robledo et al, 1999Phytotherapy Da Silva et al, 1995, Awasthi et al, 2016Nanotechnology Date et al, 2007, Alavizadeh et al, 2012, Fakhraee et al, 2016 Crucial proteins in the signaling cascade Verlinde et al, 2001, Zhai et al, 1999, Ortiz et al, 2016 Frontiers in Molecular Biosciences frontiersin.org…”

Section: Novel Development In Therapeutics Purposementioning

confidence: 99%

Systems biology of autophagy in leishmanial infection and its diverse role in precision medicine

Guhe

Ingale

Tambekar

et al. 2023

Front. Mol. Biosci.

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Autophagy is a contentious issue in leishmaniasis and is emerging as a promising therapeutic regimen. Published research on the impact of autophagic regulation on Leishmania survival is inconclusive, despite numerous pieces of evidence that Leishmania spp. triggers autophagy in a variety of cell types. The mechanistic approach is poorly understood in the Leishmania parasite as autophagy is significant in both Leishmania and the host. Herein, this review discusses the autophagy proteins that are being investigated as potential therapeutic targets, the connection between autophagy and lipid metabolism, and microRNAs that regulate autophagy and lipid metabolism. It also highlights the use of systems biology to develop novel autophagy-dependent therapeutics for leishmaniasis by utilizing artificial intelligence (AI), machine learning (ML), mathematical modeling, network analysis, and other computational methods. Additionally, we have shown many databases for autophagy and metabolism in Leishmania parasites that suggest potential therapeutic targets for intricate signaling in the autophagy system. In a nutshell, the detailed understanding of the dynamics of autophagy in conjunction with lipids and miRNAs unfolds larger dimensions for future research.

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The role of liposome–protamine–DNA nanoparticles containing CpG oligodeoxynucleotides in the course of infection induced by Leishmania major in BALB/c mice

Cited by 19 publications

References 40 publications

Nanomedical Therapeutic and Prophylaxis Strategies Against Intracellular Protozoa in the Americas

Nanomedical Therapeutic and Prophylaxis Strategies Against Intracellular Protozoa in the Americas

Enhanced immune response induced by P5 HER2/neu‐derived peptide‐pulsed dendritic cells as a preventive cancer vaccine

Systems biology of autophagy in leishmanial infection and its diverse role in precision medicine

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