The role of low-density lipoprotein receptors in sensitivity to killing by Photofrin-mediated photodynamic therapy in cultured human tumor cell lines

Misawa, Junko; Moriwaki, Shinichi; Kohno, Eiji; Hirano, Tôru; Tokura, Y.; Takigawa, Masaharu

doi:10.1016/j.jdermsci.2005.01.009

Cited by 14 publications

(8 citation statements)

References 9 publications

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“…In agreement with the proposed uptake mechanism, the fluorescence intensity at the site of the tumor increased over time as the 247/LDL complex was sequestered in tumor tissue. As the need for LDL-bound cholesterol is elevated in highly proliferative tumor cells over nontumorigenic cells, 247 has a potential application for any highly proliferative tumor (32)(33)(34)(35). Alternatively, 247 and other pzs bind to lipid vesicle models (14,22) that can act as delivery vehicles to tumors (36).…”

Section: Discussionmentioning

confidence: 99%

Chiral porphyrazine near-IR optical imaging agent exhibiting preferential tumor accumulation

Trivedi

Harney

Olive

et al. 2009

Proc. Natl. Acad. Sci. U.S.A.

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A chiral porphyrazine (pz), H 2 [pz( trans - A 2 B 2 )] (247), has been prepared that exhibits preferential in vivo accumulation in the cells of tumors. Pz 247 exhibits near-infrared (NIR) emission with λ > 700 nm in the required wavelength range for maximum tissue penetration. When MDA-MB-231 breast tumor cells are treated with 247, the agent shows strong intracellular fluorescence with an emission maximum, 704 nm, which indicates that it localizes within a hydrophobic microenvironment. Pz 247 is shown to associate with the lipophilic core of LDL and undergo cellular entry primarily through receptor-mediated endocytosis accumulating in lysosomes. Preliminary in vivo studies show that 247 exhibits preferential accumulation and retention in the cells of MDA-MB-231 tumors subcutaneously implanted in mice, thereby enabling NIR optical imaging with excellent contrast between tumor and surrounding tissue. The intensity of fluorescence from 247 within the tumor increases over time up to 48 h after injection presumably due to the sequestration of circulating 247/LDL complex by the tumor tissue. As the need for cholesterol, and thus LDL, is elevated in highly proliferative tumor cells over nontumorigenic cells, 247 has potential application for all such tumors.

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Section: Discussionmentioning

confidence: 99%

Chiral porphyrazine near-IR optical imaging agent exhibiting preferential tumor accumulation

Trivedi

Harney

Olive

et al. 2009

Proc. Natl. Acad. Sci. U.S.A.

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“…Another significant carrier molecule in the blood is LDL; it has been shown that many PS bind to LDL in circulation (65,66). Neovascular endothelial cells and tumor cells generally have a high expression of LDL receptors due to increased cell proliferation (67). Thus, hydrophilic PS bound to LDL can be preferentially accumulated into proliferating endothelial cells by the LDL receptor‐mediated endocytosis pathway.…”

Section: Enhancement Of Pdt By the Ps Conjugation To Carrier Moleculesmentioning

confidence: 99%

Strategies for Enhanced Photodynamic Therapy Effects^†

Verma

Watt

Mai

et al. 2007

Photochem & Photobiology

185

132

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Photodynamic therapy (PDT) is a treatment modality for the selective destruction of cancerous and nonneoplastic pathologies that involves the simultaneous presence of light, oxygen and a light-activatable chemical called a photosensitizer (PS) to achieve a cytotoxic effect. The photophysics and mechanisms of cell killing by PDT have been extensively studied in recent years, and PDT has received regulatory approval for the treatment of a number of diseases worldwide. As the application of this treatment modality expands with regard to both anatomical sites and disease stages, it will be important to develop strategies for enhancing PDT outcomes. This article focuses on two broad approaches for PDT enhancement: (1) mechanism-based combination treatments in which PDT and a second modality can be designed to either increase the susceptibility of tumor cells to PDT or nullify the treatment outcome-mitigating molecular responses triggered by PDT of tumors, and (2) the more recent approaches of PS targeting, either by specific cellular function-sensitive linkages or via conjugation to macromolecules.

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“…Fluence rate was measured by a UV radiometer (UVR‐2; Topcon, Tokyo, Japan). Cell survival was determined as described previously 12 . In brief, 2 × 10 4 cells were plated per 35‐mm dish and were treated with UV doses of 0–15 J/m 2 .…”

Section: Methodsmentioning

confidence: 99%

Prenatal diagnosis of xeroderma pigmentosum group A in Japan

Moriwaki¹,

Yamashita

Nakamura³

et al. 2011

The Journal of Dermatology

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We performed a prenatal diagnosis for 10 fetuses from nine unrelated Japanese xeroderma pigmentosum complementation group A (XP-A) families. All parents had at least one XP-A child (proband) with a homozygous founder mutation (IVS3-1G>C) in the XPA gene. A genetic analysis was performed by a restriction enzyme; AlwNI fragment length polymorphism of polymerase chain reaction (PCR)-amplified DNA, mostly from amniotic fluid (AF) and cultured cells established from AF. However, for the first family, we tried amniocentesis as well as chorionic villus sampling (CVS). Among the 10 cases, we confirmed the results of PCR-based genetic diagnosis by post-ultraviolet survival of amniotic cells in eight cases. Unfortunately, 6 weeks after CVS and 4 days after the amniocentesis in the first case we examined, the fetus died in utero, the reason for which remains unexplained. We prenatally determined two XP-A cases, six XP-A carriers and two wild-type fetuses, which appears to be consistent with Mendel's law.

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The role of low-density lipoprotein receptors in sensitivity to killing by Photofrin-mediated photodynamic therapy in cultured human tumor cell lines

Cited by 14 publications

References 9 publications

Chiral porphyrazine near-IR optical imaging agent exhibiting preferential tumor accumulation

Chiral porphyrazine near-IR optical imaging agent exhibiting preferential tumor accumulation

Strategies for Enhanced Photodynamic Therapy Effects^†

Prenatal diagnosis of xeroderma pigmentosum group A in Japan

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The role of low-density lipoprotein receptors in sensitivity to killing by Photofrin-mediated photodynamic therapy in cultured human tumor cell lines

Cited by 14 publications

References 9 publications

Chiral porphyrazine near-IR optical imaging agent exhibiting preferential tumor accumulation

Chiral porphyrazine near-IR optical imaging agent exhibiting preferential tumor accumulation

Strategies for Enhanced Photodynamic Therapy Effects†

Prenatal diagnosis of xeroderma pigmentosum group A in Japan

Contact Info

Product

Resources

About

Strategies for Enhanced Photodynamic Therapy Effects^†