The role of Mannheimia species in ovine mastitis

Omaleki, Lida; Browning, Glenn F.; Allen, Joanne L.; Barber, Stuart

doi:10.1016/j.vetmic.2011.03.024

Cited by 31 publications

(23 citation statements)

References 41 publications

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“…haemolytica, M. glucosida and M. ruminalis have been isolated from cases of acute mastitis in sheep (Omaleki et al, 2010(Omaleki et al, , 2011, with M. haemolytica confirmed as the most common cause of mastitis in flocks in meat type producing systems (Arsenault et al, 2008;Koop et al, 2010;Omaleki et al, 2010). In contrast, in dairy sheep, only 11% of cases of intramammary infections were found to be caused by M. haemolytica .…”

Section: Mannheimia Sppmentioning

confidence: 99%

“…Although several virulence factors of M. haemolytica have been recognised, their role in the pathogenesis of mastitis is not yet fully understood (Omaleki et al, 2011). Among these, leucotoxin produced by M. haemolytica at its logarithmic phase of growth (Zecchinon et al, 2005) has been characterised in great detail.…”

Section: Pathogenetic Processesmentioning

confidence: 99%

“…Among these, leucotoxin produced by M. haemolytica at its logarithmic phase of growth (Zecchinon et al, 2005) has been characterised in great detail. Less studied virulence factors include sialoglycerase, neuraminidase, immunoglobulin peptidase, lipopolysaccharides capsule and membrane proteins (Omaleki et al, 2011). The ruminant-specific pathogenetic action of this organism could be attributed to the selective cytotoxic action of leucotoxin against ruminant leucocytes (Zecchinon et al, 2005), causing their apoptosis (Atapattu and Czuprynski, 2005).…”

Section: Pathogenetic Processesmentioning

confidence: 99%

See 2 more Smart Citations

Mastitis in sheep – The last 10 years and the future of research

Gelasakis

Mavrogianni

Petridis

et al. 2015

Veterinary Microbiology

154

126

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Section: Mannheimia Sppmentioning

confidence: 99%

Section: Pathogenetic Processesmentioning

confidence: 99%

Section: Pathogenetic Processesmentioning

confidence: 99%

See 1 more Smart Citation

Mastitis in sheep – The last 10 years and the future of research

Gelasakis

Mavrogianni

Petridis

et al. 2015

Veterinary Microbiology

154

126

View full text Add to dashboard Cite

“…Clinical mastitis is a painful condition and can lead to the death of the animal (Arsenault et al, 2008;Omaleki et al, 2011). In dairy sheep, mastitis leads to direct losses due to reduced milk production (Cuccuru et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Comparison of bacteriological culture and PCR for detection of bacteria in ovine milk—Sheep are not small cows

Zadoks

Tassi

Martín

et al. 2014

Journal of Dairy Science

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Mastitis, inflammation of the mammary gland, is an important cause of disease, mortality, and production losses in dairy and meat sheep. Mastitis is commonly caused by intramammary infection with bacteria, which can be detected by bacterial culture or PCR. PathoProof (Thermo Fisher Scientific Ltd., Vantaa, Finland) is a commercially available real-time PCR system for the detection of bovine mastitis pathogens. Sheep differ from cattle in the bacterial species or bacterial strains that cause mastitis, as well as in the composition of their milk. The aim of this study was to evaluate whether the PathoProof system was suitable for detection of mastitis pathogens in sheep milk. Milk samples were collected aseptically from 219 udder halves of 113 clinically healthy ewes in a single flock. Aliquots were used for bacteriological culture and real-time PCR-based detection of bacteria. For species identified by culture, the diagnosis was confirmed by species-specific conventional PCR or by sequencing of a housekeeping gene. The majority of samples were negative by culture (74.4% of 219 samples) and real-time PCR (82.3% of 192 samples). Agreement was observed for 138 of 192 samples. Thirty-four samples were positive by culture only, mostly due to presence of species that are not covered by primers in the PCR system (e.g., Mannheimia spp.). Two samples were positive for Streptococcus uberis by culture but not by PCR directly from the milk samples. This was not due to inability of the PCR primers to amplify ovine Streptococcus uberis, as diluted DNA extracts from the same samples and DNA extracts from the bacterial isolates were positive by real-time PCR. For samples containing Staphylococcus spp., 11 samples were positive by culture and PCR, 9 by culture only, and 20 by PCR only. Samples that were negative by either method had lower bacterial load than samples that were positive for both methods, whereas no clear relation with species identity was observed. This study provides proof of principle that real-time PCR can be used for detection of mastitis pathogens in ovine milk. Routine use in sheep may require inclusion of primer sets for sheep-specific mastitis pathogens.

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“…These fastidious Gram-negative organisms were previously categorized within the genus Pasteurella but were reclassified in 1999 (11). Mannheimia haemolytica and M. glucosida are significant pathogens in livestock and have been associated with ovine mastitis (8,12) and pneumonia in ruminants (13). Mannheimia haemolytica has been isolated from oral and respiratory mucosae of healthy cattle and sheep.…”

mentioning

confidence: 99%

Human Wound Infection with Mannheimia glucosida following Lamb Bite

et al. 2015

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e Mannheimia spp. are veterinary pathogens that can cause mastitis and pneumonia in domestic cattle and sheep. While Mannheimia glucosida can be found as normal flora in oral and respiratory mucosa in sheep, there have been no reported cases of human infection with this organism. CASE REPORTA 64-year-old man sustained a bite on the right thumb from a 10-month-old lamb. The lamb's teeth punctured his thumbnail while he was holding the lamb's mouth open during anthelminthic treatment with a malfunctioning dosing device. He applied epoxy resin to the nail to prevent the shattered edges from catching. Following this application, he developed pain around the wound, and 10 days after the injury, he presented to the hospital. On examination, the nail was discolored, with localized wound tenderness but no discharge, and there was erythema tracking from the right thumbnail up to the axilla. The wound was explored surgically, the nail plate was removed, and pus was washed out and sent to the laboratory for microscopy and culture. The patient was treated with intravenous flucloxacillin for 24 h, followed by oral cephalexin for 7 days. On review 1 week later, the erythema had resolved and the wound had healed well.Microscopy revealed large numbers of polymorphonuclear cells, but no organisms were seen on Gram staining. After 1 day of incubation on horse blood agar, there was heavy growth of a Gram-negative bacillus that was catalase positive, oxidase positive, and indole negative. It was hemolytic on 5% horse blood agar and Mueller-Hinton agar containing 5% sheep blood. After a second day of incubation, a lighter growth of Escherichia coli, Enterococcus faecalis, coagulase-negative staphylococci, and mixed anaerobes was apparent.With the use of the Vitek 2 GN card (bioMérieux, Marcy l'Etoile, France), the Gram-negative bacillus was identified as Sphingomonas paucimobilis (97% match). Notably, the trehalose reaction was negative, the beta-glucosidase reaction was positive, and the ornithine decarboxylase reaction was negative on the Vitek card. Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS)-based identification was performed using the Microflex LT mass spectrometer (Bruker Daltonik), and the results were analyzed using the MALDI Biotyper software program (version 4.0.0.1). The top score for this Gram-negative organism was Mannheimia haemolytica, with a score of 2.177 and the accompanying comment "species of this genus have very similar patterns, therefore distinguishing their species is difficult." Other Mannheimia species appeared in the top 10 identifications listed, including Mannheimia glucosida, the highest score for which was 1.305. Partial 16S rRNA gene sequencing was performed using the MicroSeq 500 bacterial identification kit (Perkin-Elmer/Applied Biosystems, Foster City, CA), with sequence analysis performed on 500 nucleotides using MicroSeq 500 (version 2.2). A result for M. haemolytica was reported, with a specimen score of 43 and a 98.4% match (consensus length of 48...

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The role of Mannheimia species in ovine mastitis

Cited by 31 publications

References 41 publications

Mastitis in sheep – The last 10 years and the future of research

Mastitis in sheep – The last 10 years and the future of research

Comparison of bacteriological culture and PCR for detection of bacteria in ovine milk—Sheep are not small cows

Human Wound Infection with Mannheimia glucosida following Lamb Bite

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