The role of MAP-kinase p38 in the m. soleus slow myosin mRNA transcription regulation during short-term functional unloading

Sharlo, Kristina A.; Mochalova, Ekaterina P.; Belova, Svetlana P.; Lvova, Irina; Nemirovskaya, T. L.; Шенкман, Борис

doi:10.1016/j.abb.2020.108622

Cited by 11 publications

(9 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…NFATc1 exit from muscle nuclei was shown to be controlled by phosphorylation by GSK3beta and/or MAP kinase p38 [53,59,60]. The observed GSK3beta Ser 9 phosphorylation decrease followed by the kinase activation in the 7HS group as well as the prevention of this effect by PMS, corresponds to our previous data [26].…”

Section: Calcineurin/nfat Signalingsupporting

confidence: 87%

Plantar Mechanical Stimulation Maintains Slow Myosin Expression in Disused Rat Soleus Muscle via NO-Dependent Signaling

Sharlo

Paramonova

Lvova

et al. 2021

IJMS

View full text Add to dashboard Cite

It was observed that gravitational unloading during space missions and simulated microgravity in ground-based studies leads to both transformation of slow-twitch muscle fibers into fast-twitch fibers and to the elimination of support afferentation, leading to the “switching-off” of postural muscle motor units electrical activity. In recent years, plantar mechanical stimulation (PMS) has been found to maintain the neuromuscular activity of the hindlimb muscles. Nitric oxide (NO) was shown to be one of the mediators of muscle fiber activity, which can also promote slow-type myosin expression. We hypothesized that applying PMS during rat hindlimb unloading would lead to NO production upregulation and prevention of the unloading-induced slow-to-fast fiber-type shift in rat soleus muscles. To test this hypothesis, Wistar rats were hindlimb suspended and subjected to daily PMS, and one group of PMS-subjected animals was also treated with nitric oxide synthase inhibitor (L-NAME). We discovered that PMS led to sustained NO level in soleus muscles of the suspended animals, and NOS inhibitor administration blocked this effect, as well as the positive effects of PMS on myosin I and IIa mRNA transcription and slow-to-fast fiber-type ratio during rat hindlimb unloading. The results of the study indicate that NOS activity is necessary for the PMS-mediated prevention of slow-to-fast fiber-type shift and myosin I and IIa mRNA transcription decreases during rat hindlimb unloading.

show abstract

Section: Calcineurin/nfat Signalingsupporting

confidence: 87%

Plantar Mechanical Stimulation Maintains Slow Myosin Expression in Disused Rat Soleus Muscle via NO-Dependent Signaling

Sharlo

Paramonova

Lvova

et al. 2021

IJMS

View full text Add to dashboard Cite

show abstract

“…Transcription of myh7b mRNA (as well as MyHC I) is significantly reduced in rat soleus muscles after 24 h of HS [ 121 ] and remains decreased after 3 and 7-day HS. SOX6 mRNA elevates after the 3rd day of HS and remains increased after 7-day unloading [ 66 , 122 ]. It was shown that overexpression of microRNA-499 in C2C12 myotubes leads to an increased expression of the slow isoform of troponin (TNNI1), myoglobin, and PGC-1α, thus this microRNA plays an important role in the realization of the oxidative phenotype of muscle fiber (Xu, Chen et al 2018).…”

Section: Impact Of Gsk-3β Activity On Fiber-type Transitions and Oxidative Capacity In Skeletal Muscles Under Mechanical Unloading And Sumentioning

confidence: 99%

The Role of GSK-3β in the Regulation of Protein Turnover, Myosin Phenotype, and Oxidative Capacity in Skeletal Muscle under Disuse Conditions

Mirzoev

Sharlo

Шенкман

2021

IJMS

View full text Add to dashboard Cite

Skeletal muscles, being one of the most abundant tissues in the body, are involved in many vital processes, such as locomotion, posture maintenance, respiration, glucose homeostasis, etc. Hence, the maintenance of skeletal muscle mass is crucial for overall health, prevention of various diseases, and contributes to an individual’s quality of life. Prolonged muscle inactivity/disuse (due to limb immobilization, mechanical ventilation, bedrest, spaceflight) represents one of the typical causes, leading to the loss of muscle mass and function. This disuse-induced muscle loss primarily results from repressed protein synthesis and increased proteolysis. Further, prolonged disuse results in slow-to-fast fiber-type transition, mitochondrial dysfunction and reduced oxidative capacity. Glycogen synthase kinase 3β (GSK-3β) is a key enzyme standing at the crossroads of various signaling pathways regulating a wide range of cellular processes. This review discusses various important roles of GSK-3β in the regulation of protein turnover, myosin phenotype, and oxidative capacity in skeletal muscles under disuse/unloading conditions and subsequent recovery. According to its vital functions, GSK-3β may represent a perspective therapeutic target in the treatment of muscle wasting induced by chronic disuse, aging, and a number of diseases.

show abstract

“…P38 MAPK plays a critical role in muscle regeneration [ 34 ]. It was first described as a transducer of the response to environmental stress conditions, and in muscle was found to regulate muscle fiber formation via satellite cell differentiation, slow myosin heavy chain gene repression [ 35 ], and is involved in muscle pathologies [ 36 ]. When taken into context with our observed increase in fast MyHC, centrally located nuclei, and RAGE signaling, use of p38 MAPK as a marker of regeneration in a VML model becomes more intriguing.…”

Section: Discussionmentioning

confidence: 99%

Human Adipose-Derived Stromal Cells Delivered on Decellularized Muscle Improve Muscle Regeneration and Regulate RAGE and P38 MAPK

et al. 2022

View full text Add to dashboard Cite

Volumetric muscle loss (VML) is the acute loss of muscle mass due to trauma. Such injuries occur primarily in the extremities and are debilitating, as there is no clinical treatment to restore muscle function. Pro-inflammatory advanced glycation end-products (AGEs) and the soluble receptor for advanced glycation end-products (RAGE) are known to increase in acute trauma patient’s serum and are correlated with increased injury severity. However, it is unclear whether AGEs and RAGE increase in muscle post-trauma. To test this, we used decellularized muscle matrix (DMM), a pro-myogenic, non-immunogenic extracellular matrix biomaterial derived from skeletal muscle. We delivered adipose-derived stromal cells (ASCs) and primary myoblasts to support myogenesis and immunomodulation (N = 8 rats/group). DMM non-seeded and seeded grafts were compared to empty defect and sham controls. Then, 56 days after surgery muscle force was assessed, histology characterized, and protein levels for AGEs, RAGE, p38 MAPK, and myosin heavy chains were measured. Overall, our data showed improved muscle regeneration in ASC-treated injury sites and a regulation of RAGE and p38 MAPK signaling, while myoblast-treated injuries resulted in minor improvements. Taken together, these results suggested that ASCs combined with DMM provides a pro-myogenic microenvironment with immunomodulatory capabilities and indicates further exploration of RAGE signaling in VML.

show abstract

The role of MAP-kinase p38 in the m. soleus slow myosin mRNA transcription regulation during short-term functional unloading

Cited by 11 publications

References 26 publications

Plantar Mechanical Stimulation Maintains Slow Myosin Expression in Disused Rat Soleus Muscle via NO-Dependent Signaling

Plantar Mechanical Stimulation Maintains Slow Myosin Expression in Disused Rat Soleus Muscle via NO-Dependent Signaling

The Role of GSK-3β in the Regulation of Protein Turnover, Myosin Phenotype, and Oxidative Capacity in Skeletal Muscle under Disuse Conditions

Human Adipose-Derived Stromal Cells Delivered on Decellularized Muscle Improve Muscle Regeneration and Regulate RAGE and P38 MAPK

Contact Info

Product

Resources

About