2016
DOI: 10.1107/s2053230x16000030
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The role of mass transport in protein crystallization

Abstract: Mass transport takes place within the mesoscopic to macroscopic scale range and plays a key role in crystal growth that may affect the result of the crystallization experiment. The influence of mass transport is different depending on the crystallization technique employed, essentially because each technique reaches supersaturation in its own unique way. In the case of batch experiments, there are some complex phenomena that take place at the interface between solutions upon mixing. These transport instabiliti… Show more

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Cited by 17 publications
(27 citation statements)
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“…Data and information obtained from crystallographic investigations are important for macromolecular engineering to optimize biomolecules for various applications in biomedical research. Macromolecular transport in crystallization processes has been shown to directly affect crystal quality (García-Ruiz et al, 2016;McPherson et al, 1999;Vekilov, 1999). Gravity-dependent flow effects, including convection and sedimentation, affect the crystal growth processes on earth (Lee and Chernov, 2002;Otálora et al, 2001;Wilcox, 1983).…”
Section: Introductionmentioning
confidence: 99%
“…Data and information obtained from crystallographic investigations are important for macromolecular engineering to optimize biomolecules for various applications in biomedical research. Macromolecular transport in crystallization processes has been shown to directly affect crystal quality (García-Ruiz et al, 2016;McPherson et al, 1999;Vekilov, 1999). Gravity-dependent flow effects, including convection and sedimentation, affect the crystal growth processes on earth (Lee and Chernov, 2002;Otálora et al, 2001;Wilcox, 1983).…”
Section: Introductionmentioning
confidence: 99%
“…Figure 2 shows representative crystals of insulin (model protein) and oxy-hemoglobin II, II-III (target protein) grown both in space and on-ground. For structural biology studies, the best protein crystals are those producing the better diffraction data, in order to accurately determine the 3D structural model of the target protein [6,18]. As a rule of thumb, the quality of a crystal depends on two key factors: the coherent attachment of the molecules to the crystal lattice and the amount of impurity incorporated in the crystal volume, which can produce lattice strain [50].…”
Section: Resultsmentioning
confidence: 99%
“…As a rule of thumb, the quality of a crystal depends on two key factors: the coherent attachment of the molecules to the crystal lattice and the amount of impurity incorporated in the crystal volume, which can produce lattice strain [50]. The first factor is directly related to the supersaturation and supersaturation rate, and a correlation has been found between low supersaturation and better lattice For structural biology studies, the best protein crystals are those producing the better diffraction data, in order to accurately determine the 3D structural model of the target protein [6,18]. As a rule of thumb, the quality of a crystal depends on two key factors: the coherent attachment of the molecules to the crystal lattice and the amount of impurity incorporated in the crystal volume, which can produce lattice strain [50].…”
Section: Resultsmentioning
confidence: 99%
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