The role of mast cells in lymphangiogenesis in psoriasis

Abstract

Introduction. Lymphangiogenesis is a complex process mediated by a wide spectrum of inflammatory cells including mast cells. Lymphatic vessels play an important role in chronic inflammatory disorders including psoriasis. Objective. Assessment of interaction between lymphatic vessels and inflammatory cells, including mast cells to determine the influence of inflammatory cells on lymphangiogenesis in psoriasis. Material and methods. Fifty patients with psoriasis and 10 healthy controls were included. D2-40 immunostaining was performed for delineation of lymphatic vessels, and Giemsa stain for highlighting mast cells. Results. Psoriatic skin lesions were characterized by a higher number of mast cells and inflammatory cells as well as denser and larger lymphatic vessels than normal skin. A significant positive correlation was detected between number of lymphatic vessels and mast cells as well as other inflammatory cells in psoriatic lesions. However, none of them emerged as an independent inducer of lymphangiogenesis on multilogistic regression analysis. Simple logistic regression analysis revealed that mast cells are significant influencers. One unit change in mast cells can explain 1.369 unit changes in lymphatic vessels, while one unit change in other inflammatory cells can only explain 0.099 unit change in lymphatic vessels. Conclusions. Mast cells and other inflammatory cells significantly and synergistically act in induction of lymphangiogenesis in psoriatic lesions, with a slightly higher participation for mast cells. streszczenie Wprowadzenie. Limfangiogeneza jest złożonym procesem, w którym uczestniczy szerokie spektrum komórek zapalnych, w tym komórki tuczne. Naczynia limfatyczne odgrywają istotną rolę w przewlekłych chorobach zapalnych, m.in. w łuszczycy. Cel pracy. Ocena interakcji pomiędzy naczyniami limfatycznymi a komórkami zapalnymi, w tym komórkami tucznymi, w celu określenia wpływu komórek zapalnych na proces limfangiogenezy w łuszczycy. Materiał i metody. Badanie objęło 50 pacjentów z łuszczycą i 10 zdrowych osób, które stanowiły grupę kontrolną. Do wizualizacji naczyń limfatycznych wykorzystano barwienie immunohistochemiczne z przeciwciałem D2-40, a do oznaczenia komórek tucznych-barwienie metodą Giemsy.

Assessing the conformity of single-use food plastic packaging products to the applicable standards: A seven-year study of tests conducted at the Ghana Standards Authority

“…3.1.4 Tensile Strength and Elongation at BreakPlastic lms have high mechanical properties such as tensile strength, tear strength, and impact resistance, which usually depend on the molecular structure, molecular mass, and molar mass distribution of the plastics (Abdel-Bary, 2003). Based on the study, it was observed that the HDPE, LDPE, and recycled PE Carrier Bag plastic products demonstrated overall conformity to the Tensile Strength and Elongation at Break requirements speci ed in the standard GS 173:2018.The average Tensile Strength (Machine) results of 44.3MPa (SD = 15.6), 41.6MPa (SD = 58.0), and 20.48MPa (SD = 11.7) for HDPE, LDPE, and recycled PE Carrier Bag plastic products, respectively, were recorded.…”