The role of mesenchymal stromal cells in the repair of acute organ injury

Temnov, A. A.; Вагабов, А. В.; Sklifas, A. N.; Новоселов, В. И.; Belyi, Yu. A.

doi:10.1002/9781118907474.ch35

Cited by 2 publications

(1 citation statement)

References 203 publications

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“… 10 As has been shown in previous experiments, paracrine factors secreted by cultured stem cells are effective in treating acute chemical and thermal burns of the skin and mucous membranes. 11 , 12 Keeping in adherence to this, the aim of our experiment was to study the effect/impact of paracrine factors taken from stem cells from the bone marrow of rats in the treatment of local radiation burns.…”

Section: Introductionmentioning

confidence: 99%

Use of paracrine factors from stem cells to treat local radiation burns in rats

Temnov

Астрелина

Rogov

et al. 2018

SCCAA

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BackgroundMesenchymal stem cells based paracrine bioactive factors that deploy their task as an essential mechanism, but their efficiency for skin regeneration still requires clarification.MethodsThe mesenchymal stem cell-based paracrine factors were administered by subcutaneous injection of 0.5 mL peptides (general protein 8 mg/mL). These were performed after radiation on different days like the first, third, sixth, eighth, and 10th. To determine the consequences, we performed photography, planimetry, and preclinical test each week after 15 days of radiation. MSC-based peptides were injected into a rat that had radiation burns, and its observation encouraged cell-free therapeutic remedies to regenerate skin. Both control and experimental groups were exposed to 110 Gy of X-rays, which resulted in the formation of localized radiation burns on the skin (S=6 cm2) 15 days later. Thirty days after radiation, the wound stabilized (surface of the wound was S=2.2±0.2 cm2) and fluctuated throughout the course of the pathological process.ResultsThe wounded area on the skin from the 15th to the 29th day after radiation was practically the same in both groups. The wounded area gradually reduced by 6.1±0.4 cm2 (experimental group) and 5.9±0.6 cm2 (control group) 15 days after radiation up to 2.2±0.3 cm2 (in both control and experimental groups) on the 29th day after radiation. However, starting from the 36th day, there was a constant reduction in the burn area in the experimental group up to 0.2±0.1 cm2 till the 71st day after radiation.ConclusionIn the control group, the area of the lesion ranged from 1.4±0.6 cm2 on the 50th day to 1.9±0.8 cm2 on the 71st day. During the 57th to the 71st day, the difference between the affected area in the experimental and control groups was 1:8. The experimental group has a significantly higher level of skin regeneration and significant decrease in the level of leukocyte infiltration, thereby reducing necrosis.

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Section: Introductionmentioning

confidence: 99%

Use of paracrine factors from stem cells to treat local radiation burns in rats

Temnov

Астрелина

Rogov

et al. 2018

SCCAA

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Effect of conditioned medium from mesenchymal stem cells on regeneration of endothelium at HCl-induced damage trachea in rats

Temnov

Волкова

Melerzanov

et al. 2017

ZHurnal «Patologicheskaia Fiziologiia I Eksperimental`naia Terapiia»

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Цель - изучение регенерации респираторного эпителия крысы при ингаляционной травме трахеи парами соляной кислоты. Методика. Активация процессов регенерации проводилась после химического ожога путем аппликации в трахею кондиционированной среды, полученной при культивировании аллогенных мезенхимальных стволовых клеток (кМСК). Результаты. Показано, что на 3-и 7-е сут. после аппликации препарата кМСК эпителий трахеи практически полностью восстанавливается. При этом в процессе регенерации в подслизистом слое эпителия образуются замкнутые структуры, содержащие цилиарные клетки, аналогичные реснитчатым клеткам респираторного эпителия трахеи. Эти структуры мигрируют в сторону эпителия и встраиваются в пораженный эпителий. Заключенние. Показано, что препарат кМСК, введенный непосредственно в трахею в ранние сроки после повреждения, способствует восстановлению нормального подслизистого слоя. Было отмечено снижение выраженности воспаления, ускорение темпов миграции цилиарных клеток к поверхности трахеи и формирование реснитчатого эпителия de novo. The purpose. Respiratory epithelium regeneration is studied in rats with tracheal damage induced by inhaling hydrochloric acid vapor. Method. Regeneration process after the chemical burn was activated by intratracheal administration of preparations obtained from the same-species mesenchymal stem cells (MSC). Results. Tracheal epithelium is shown to recover almost completely on day 3-7 after applying MSC compositions (MSCs). Closed structures containing ciliated cells similar to ciliated cells of the respiratory epithelium lining the trachea are formed in the submucosal epithelium during regeneration. These structures migrate towards epithelium and get incorporated into the damaged epithelium. This phenomenon is apparently indicative of the special mechanism of respiratory epithelium regeneration after HCl-induced injury. Conclusion. It is demonstrated in this study that cell-free MSCs instilled intratracheally promote the recovery of normal submucosal epithelium by either preventing or reducing necrosis and inflammation. Such topical MSCs administration significantly accelerates migration of ciliated cell towards the surface and de novo formation of the ciliary epithelium.

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The role of mesenchymal stromal cells in the repair of acute organ injury

Cited by 2 publications

References 203 publications

Use of paracrine factors from stem cells to treat local radiation burns in rats

Use of paracrine factors from stem cells to treat local radiation burns in rats

Effect of conditioned medium from mesenchymal stem cells on regeneration of endothelium at HCl-induced damage trachea in rats

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