The Role of MHC Class I Expression in Rat NK Cell-Mediated Lysis of Syngeneic Tumor Cells and Virus-Infected Cells

Giezeman‐Smits, Katinka M.; Kuppen, Peter J.K.; Ensink, N. G.; Eggermont, Alexander M.M.; Stals, Frans; Wonigeit, K.; Fleuren, Gert Jan

doi:10.1016/s0171-2985(96)80046-5

Cited by 12 publications

(6 citation statements)

References 29 publications

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“…A-NK cells lysed CC531 tumor cells up to about 50% in vitro in a 19 hr 51 Cr-release assay in the presence of 1,000 CU IL-2/ml at an effector-to-target ratio of 20:1 (Giezeman- Smits et al, 1996). To test whether such in vitro lytic capacity was maintained in vivo, a Winn-type assay was performed in which both A-NK cells and CC531 tumor cells were injected subcapsularly into the liver at an effector-to-target-ratio of 10:1.…”

Section: Anti-tumor Effects Of Bolus A-nk Cells Injectionmentioning

confidence: 99%

Regional administration of natural killer cells in a rat hepatic metastasis model results in better tumor infiltration and anti-tumor response than systemic administration

et al. 1998

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Section: Anti-tumor Effects Of Bolus A-nk Cells Injectionmentioning

confidence: 99%

Regional administration of natural killer cells in a rat hepatic metastasis model results in better tumor infiltration and anti-tumor response than systemic administration

et al. 1998

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“…NK cells, derived from WAG rats (Charles River), were isolated and cultured as previously described [14,15]. NK cell line NKbr11.3 and four NK clones were established and maintained as previously described [16].…”

Section: Cell Culturementioning

confidence: 99%

“…Nylon wool nonadherent cells (NWNAC) were isolated as previously described [14,15]. They were cultured for 9 days in CM in the presence of 1000 U/mL human recombinant IL-2 (Chiron, Amsterdam, The Netherlands) and 50 µM 2-ME.…”

Section: In Vitro Induction Of Nk Cell Surface Antigens By Il-2mentioning

confidence: 99%

“…The expression of cell surface antigens on freshly isolated lymphocytes was measured using flow cytometry by double staining of freshly isolated splenocytes using 3.2.3 (IgG2b) for NK cells; R73 (IgG2b) for T cells; and goat-anti-rat-IgG (SBA, Birmingham, AL) for B cells. ␥␦T cells were freshly isolated by immunomagnetic separation [15] using the antibody V65.…”

Section: Immunostainingmentioning

confidence: 99%

“…NK cell-mediated lysis of target cells was measured with a 51 Cr release assay as described [15]. To test the effect of the antibodies on lysis, the antibodies were added to the assay in 50 µL in the final concentration of 4 µg/mL.…”

Section: Cr Release Assaymentioning

confidence: 99%

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Novel monoclonal antibodies against membrane structures that are preferentially expressed on IL-2-activated rat NK cells

Giezeman‐Smits

Jonges

Chambers

et al. 1998

Journal of Leukocyte Biology

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Interleukin-2 (IL-2)-activated natural killer (NK) cells are known to mediate specific functions such as cytolytic activity and tumor infiltration more efficiently than nonactivated NK cells. To investigate whether these characteristics are associated with induction or up-regulation of expression of membrane structures after IL-2 activation, we selected four hybridomas (mAbs ANK44, ANK66, ANK7, and ANK123) derived from mice immunized with rat IL-2-activated NK cells and compared the expression of the epitopes recognized on IL-2-activated NK cells versus unstimulated NK cells. We found that ANK44-expression was induced after activation with IL-2. The antigens recognized by ANK66, ANK7, and ANK123 were expressed selectively on subsets of nonactivated NK cells. After activation with IL-2 the level of expression of these antigens was increased. Moreover, the majority of NK cells then expressed these antigens after IL-2 activation. Biochemical characterization of the membrane structures recognized on IL-2-activated NK cells showed that they have not previously been described. The precise function of these membrane structures is not yet known, however, the selective nature of their expression implies their involvement in the specific functions of IL-2-activated NK cells.

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Rat Hepatic Natural Killer Cells (Pit Cells) Express mRNA and Protein Similar to in Vitro Interleukin-2 Activated Spleen Natural Killer Cells

Luo

Vanderkerken

Chen

et al. 2001

Cellular Immunology

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The Role of MHC Class I Expression in Rat NK Cell-Mediated Lysis of Syngeneic Tumor Cells and Virus-Infected Cells

Cited by 12 publications

References 29 publications

Regional administration of natural killer cells in a rat hepatic metastasis model results in better tumor infiltration and anti-tumor response than systemic administration

Regional administration of natural killer cells in a rat hepatic metastasis model results in better tumor infiltration and anti-tumor response than systemic administration

Novel monoclonal antibodies against membrane structures that are preferentially expressed on IL-2-activated rat NK cells

Rat Hepatic Natural Killer Cells (Pit Cells) Express mRNA and Protein Similar to in Vitro Interleukin-2 Activated Spleen Natural Killer Cells

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