The role of microbiota-derived postbiotic mediators on biofilm formation and quorum sensing-mediated virulence of Streptococcus mutans: A perspective on preventing dental caries

OmerOglou, Emine; Karaca, Başar; Kibar, Hazal; Haliscelik, Ozan; Kıran, Fadime

doi:10.1016/j.micpath.2022.105390

Cited by 26 publications

(41 citation statements)

References 42 publications

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“…Dental caries can adversely impact the quality of life of affected patients, resulting in complications including pain and, in severe cases, tooth loss (Aldosari et al, 2021;Amarasena et al, 2021;Santos et al, 2021). Streptococcus mutans (S. mutans) is an essential cariogenic bacterium that metabolizes carbohydrates within the oral cavity and thereby lowers the local pH at the tooth surface (Loesche, 1986;OmerOglou et al, 2022). When this pH value drops to 5.5 or below, it can result in tooth surface demineralization and dental caries development (He et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

Genomic and phenotypic characterization of Streptococcus mutans isolates suggests key gene clusters in regulating its interaction with Streptococcus gordonii

et al. 2022

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Streptococcus mutans (S. mutans) is one of the primary pathogens responsible for dental caries. Streptococcus gordonii (S. gordonii) is one of the early colonizers of dental plaque and can compete with S. mutans for growth. In the present analysis, we explored key target genes against S. gordonii in S. mutans using 80 S. mutans clinical isolates with varying capabilities against S. gordonii. A principal coordinate analysis revealed significant genetic diversity differences between antagonistic and non-antagonistic groups. Genomic comparisons revealed 33 and 61 genes that were, respectively, positively and negatively correlated with S. mutans against S. gordonii, with RNA-sequencing (RNA-seq) highlighting 11 and 43 genes that were, respectively, upregulated and downregulated in the antagonistic group. Through a combination of these results and antiSMASH analysis, we selected 16 genes for qRT-PCR validation in which the expression levels of SMU_137 (malate dehydrogenase, mleS), SMU_138 (malate permease, mleP), SMU_139 (oxalate decarboxylase, oxdC), and SMU_140 (glutathione reductase) were consistent with RNA-seq results. SMU_1315c-1317c (SMU_1315c transport-related gene) and SMU_1908c-1909c were, respectively, downregulated and upregulated in the antagonistic group. The expression patterns of adjacent genes were closely related, with correlation coefficient values greater than 0.9. These data reveal new targets (SMU_137–140, SMU_1315c-1317c, and SMU_1908c-1909c) for investigating the critical gene clusters against S. gordonii in S. mutans clinical isolates.

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Section: Introductionmentioning

confidence: 99%

Genomic and phenotypic characterization of Streptococcus mutans isolates suggests key gene clusters in regulating its interaction with Streptococcus gordonii

et al. 2022

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“…This is the first attempt for alleviation of S. mutans using beneficial bacterial isolate E. cloacae PS‐74, although several Lactobacillus sp. have been reported on the effectiveness of probiotics on cariogenic pathogens from different sources 6 . The morphological, physiochemical, and biochemical characterization of E. cloacae PS‐74 are listed in Table 3.…”

Section: Resultsmentioning

confidence: 99%

“…Various studies have discovered that probiotics or cell‐free supernatants (CFS) from different commercial strains can prevent tooth infections 6,7,8 . The effectiveness of lactic acid bacteria (LAB) has been reported for eliminating the dental pathogen 9,12 …”

Section: Introductionmentioning

confidence: 99%

Alleviation of dental caries by use of isolated potential probiotic and its characterization

Pallavi

Barik

Sahoo

et al. 2023

Biotech and App Biochem

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Streptococcus mutans plays a major role in biofilm formation and pathogenic bacterial adhesion. Here we investigated the abilities of our isolates from diverse conventional sources to characterize the beneficial bacteria for inhibition of S. mutans. Enterobacter cloacae PS‐74, a beneficial bacteria isolated from yoghurt, is gram negative, rod shaped, and resistant to acid, bile salt, and amylase. PS‐74 cell‐free supernatants (CFS) demonstrated highest zone of inhibition of 29 ± 1.7 mm. Further, the minimum inhibitory concentration (MIC) value of CFS PS‐74 was recorded to be 10 μL and its minimum bactericidal concentration (MBC) value was found to be 15μL which led to 99.9% log reduction of S. mutans. Moreover, the biofilm formation was reduced by 84.91% at MIC15 of CFS PS‐74 which alleviate the dental caries formation by S. mutans. This is the first report on E. cloacae PS‐74, which was studied for its probiotic properties to inhibit S. mutans MTCC‐890 due to the production of organic acids and employed in oral treatment.

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“…The common dietary risk factor for caries and other chronic diseases is the high and frequent consumption of fermentable carbohydrates, particularly ultra-processed foods which have high amounts of refined starches and free sugars and are associated with eating patterns that exceed recommended sugar consumption levels of less than 10% of total energy intake [ 9 , 10 , 11 , 12 ]. Upon ingestion of these foods, endogenous bacteria in the biofilms produce organic acids as a by-product of the metabolism of fermentable carbohydrates [ 13 , 14 , 15 ]. These lactic, formic, and acetic acids cause local pH values in the dental biofilm to fall below neutrality to critical values of 5.5 and below, which results in the demineralisation of tooth tissues.…”

Section: Introductionmentioning

confidence: 99%

Healthy Food, Healthy Teeth: A Formative Study to Assess Knowledge of Foods for Oral Health in Children and Adults

2022

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Eating patterns characterised by low intakes of processed carbohydrates and higher intakes of fat- and Vitamin D-rich foods are associated with protection against dental caries. The aim of this formative study was to evaluate the extent to which the knowledge of children and adults of foods for oral health reflects dietary guideline advice, and the evidence base for foods associated with increased and decreased caries burdens. Using a novel card-sorting task, the participants categorised foods according to their knowledge of each food for oral health. There were no differences between children and adults in the categorisation of fresh, minimally processed foods. Fish, chicken, and red meat were categorised as healthy by significantly fewer children than adults. High-sugar foods were correctly characterised as unhealthy by nearly all participants. More children categorised breakfast cereals as healthy than adults. There were no statistically significant differences between children and adults for the categorisation of brown or wholegrain breads categorised as healthy. The alignment of the participants’ beliefs with dietary guideline recommendations suggests education through health promotion initiatives is successful in achieving knowledge acquisition in children and adults. However, recommendations to increase the intake of refined carbohydrates inadvertently advocate foods associated with increased caries burdens.

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The role of microbiota-derived postbiotic mediators on biofilm formation and quorum sensing-mediated virulence of Streptococcus mutans: A perspective on preventing dental caries

Cited by 26 publications

References 42 publications

Genomic and phenotypic characterization of Streptococcus mutans isolates suggests key gene clusters in regulating its interaction with Streptococcus gordonii

Genomic and phenotypic characterization of Streptococcus mutans isolates suggests key gene clusters in regulating its interaction with Streptococcus gordonii

Alleviation of dental caries by use of isolated potential probiotic and its characterization

Healthy Food, Healthy Teeth: A Formative Study to Assess Knowledge of Foods for Oral Health in Children and Adults

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