The role of microRNAs in the healing of diabetic ulcers

Goodarzi, Golnaz; Maniati, Mahmood; Qujeq, Durdi

doi:10.1111/iwj.13070

Cited by 26 publications

(21 citation statements)

References 171 publications

(401 reference statements)

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“…3 In recent years, an increasing number of studies have shown that abnormal expression of microRNAs (miRNAs) is closely associated with the occurrence and prognosis of DFU. 4 MiRNAs are a class of endogenous non-coding RNAs with a length of approximately 18-25 nucleotides. They can regulate the expression of a target gene by specifically binding to the 3′ untranslated region of downstream target mRNA to guide the silencing complex to degrade mRNA or…”

Section: Introductionmentioning

confidence: 99%

Increased Expression of miR-155 in Peripheral Blood and Wound Margin Tissue of Type 2 Diabetes Mellitus Patients Associated with Diabetic Foot Ulcer

Xu¹,

Li²,

Tang³

et al. 2022

DMSO

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To investigate the correlations of miR-155 expression in the peripheral blood and wound margin tissue of patients with diabetic foot ulcer (DFU) and explore the clinical value of miR-155 as a potential biomarker for the diagnosis and treatment outcomes of DFU. Methods: Sixty newly diagnosed T2DM patients without DFU (T2DM group), 112 T2DM patients with DFU (DFU group), and 60 healthy controls (NC group) were included. MiR-155 levels in the peripheral blood and wound margin tissue were determined by quantitative real-time PCR, while clinical features and risk factors of DFU were explored. Multiple stepwise logistic regression analysis was used to determine whether miR-155 expression was an independent risk factor for DFU. The diagnostic effectiveness of miR-155 level on DFU was evaluated using ROC curve analysis. Results: A significant decrease in the expression level of miR-155 was observed in T2DM group compared with NC group (P < 0.05), while a markedly increased miR-155 expression level was noted in DFU group compared with T2DM group (P < 0.01). Moreover, there was a negative correlation between the expression levels of miR-155 with healing rate of DFU. Kaplan-Meier survival curve analysis showed that the cumulative rate of unhealed DFU in miR-155 high expression group is higher than that in miR-155 low expression group, both in peripheral blood and wound margin tissue (log rank, P = 0.004, P < 0.001, respectively). The multivariate logistic regression analysis confirmed that a high expression of miR-155 was an independent risk factor for DFU. The ROC curve analysis indicated that the AUC of miR-155 for the diagnosis of DFU was 0.794, with the optimum sensitivity being 96.82% and the optimum specificity of 95.93%. Conclusion:The increased expression of miR-155 in peripheral blood of T2DM patients is closely related to the occurrence of DFU. MiR-155 is a potentially valuable biomarker for diagnosis and prognosis of DFU.

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Section: Introductionmentioning

confidence: 99%

Increased Expression of miR-155 in Peripheral Blood and Wound Margin Tissue of Type 2 Diabetes Mellitus Patients Associated with Diabetic Foot Ulcer

Xu¹,

Li²,

Tang³

et al. 2022

DMSO

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“… 3 A body of studies have found that the abnormal expression of miRNAs is associated with the occurrence and development of many diseases, including the healing of diabetic foot ulcers and the regulation of bone formation. 4 , 5 MiR-34c is a member of the miR-34 family and has been extensively explored in tumor formation, 6 renal fibrosis, 7 Alzheimer’s disease, 8 and so forth. Recent studies have shown 9 that miR-34c can aggravate chronic wound inflammation, inhibit the proliferation and migration of keratinocytes, promote their apoptosis, and hinder the re-epithelialization of wounds.…”

Section: Introductionmentioning

confidence: 99%

Enhanced Expression of miR-34c in Peripheral Plasma Associated with Diabetic Foot Ulcer in Type 2 Diabetes Patients

Wu¹,

Xie²,

Zhao³

et al. 2021

DMSO

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To explore the correlation between the expression of miR-34c in peripheral blood of patients with type 2 diabetes mellitus (T2DM) and the onset of diabetic foot ulcer (DFU) and diabetic foot osteomyelitis (DFO). Methods: Sixty newly diagnosed patients with T2DM without DFU (T2DM group), 112 T2DM patients with DFU (DFU group) and 60 controls with normal glucose tolerance (NC group). The DFU group patients were subdivided into DFO (n=64) and NDFO (n=48) groups. Quantitative real-time PCR (qRT-PCR) method was used to determine miR-34c expression levels in the peripheral blood of subjects to analyze the clinical characteristics of DFU and DFO risk factors. Results: MiR-34c expression level in the T2DM group was marked higher than the NC group [2.99 (1.45-6.22) vs 1.01 (0.89-1.52)] (P < 0.05). However, the expression level of miR-34c in the DFU group was significantly higher than the T2DM group [9.65 (6.15-18.63) vs 2.99 (1.45-6.22)] (P < 0.01). Compared with the NDFO group, the expression level of miR-34c in the DFO group was also obviously increased [13.46 (8.89-19.11) vs 6.02 (5.93-14.72)] (P < 0.01). The expression level of miR-34c in DFU patients was positively correlated with the amputation rate of foot ulcers (P=0.030) and was negatively correlated with the healing rate of foot ulcers after eight weeks (P=0.025). Multifactorial logistic regression analysis showed that increased expression of miR-34c was an independent risk factor for DFU and DFO (OR DFU =3.47, OR DFO =4.25, P < 0.01). Meanwhile, the ROC curve analysis indicated that the AUC of miR-34c for the diagnosis of DFU and DFO was 0.803 and 0.904, the optimum sensitivity being was 100% and 98.7%, the optimum specificity was 98.4% and 98.4%, respectively. Conclusion:The increased expression of miR-34c in peripheral blood of T2DM patients is closely related to the occurrence, development and prognosis of DFU and DFO.

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“…MicroRNAs (miRNAs), endogenous small noncoding RNAs 18‐25 nucleotides long, played a significant role in the regulation of gene expression by specifically binding with the 3′‐UTR region of downstream target messenger RNA (mRNA), which results to the inhibition of mRNA translation or direct degradation of miRNA 4 . Numerous studies have established the relationship between abnormally expressed miRNAs and the occurrence and development of many diseases, including the healing process of DFU and the regulatory process of bone formation 5‐7 . Furthermore, miR‐24 was an abundantly expressed and highly conservative miRNA put together with miR‐23 and MiR‐27 on human chromosomes 9 and 19 8 .…”

Section: Introductionmentioning

confidence: 99%

Decreased expression of miR‐24 in peripheral plasma of type 2 diabetes mellitus patients associated with diabetic foot ulcer

Tang

Jia

et al. 2020

Wound Repair Regeneration

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To examine the correlations of miR‐24 expression in peripheral plasma with the onset of diabetic foot ulcer (DFU) and diabetic foot osteomyelitis (DFO) in type 2 diabetes mellitus (T2DM) patients and explore the clinical value of miR‐24 as a potential biomarker for the diagnosis and treatment outcomes of DFU and DFO, a total of 60 newly diagnosed T2DM patients without DFU (T2DM group), 112 T2DM patients with DFU (DFU group), and 60 healthy controls (NC group) were included. DFU group were further divided into DFO group (n = 64) and non‐DFO group (n = 48). MiR‐24 levels were determined by quantitative real‐time PCR, while clinical features and risk factors of DFU and DFO were explored. The expression level of miR‐24 in T2DM and DFU group was significantly lower than in NC group (P < .05), and that in DFU group was significantly lower than in T2DM group (P < .01). Additionally, the level of miR‐24 significantly decreased in DFO group compared to non‐DFO group (P < .01). Moreover, it was negatively correlated with the amputation rate in DFU group (P = .043) and positively correlated with healing rate after 8 weeks (P = .036). The multivariate logistic regression analysis confirmed that a low expression of miR‐24 was an independent risk factor for DFU and DFO. The ROC curve analysis indicated that the AUC of miR‐24 for the diagnosis of DFU and DFO was 0.849 (95% CI, 0.618‐0.879, P < .001) and 0.782 (95% CI, 0.595‐0.813, P < .001). Thus, a decreased expression of miR‐24 of T2DM patients was closely related to the occurrence, development and prognosis of DFU and DFO, suggesting the use of miR‐24 as a potential biomarker for the prediction of DFU and DFO.

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The role of microRNAs in the healing of diabetic ulcers

Cited by 26 publications

References 171 publications

Increased Expression of miR-155 in Peripheral Blood and Wound Margin Tissue of Type 2 Diabetes Mellitus Patients Associated with Diabetic Foot Ulcer

Increased Expression of miR-155 in Peripheral Blood and Wound Margin Tissue of Type 2 Diabetes Mellitus Patients Associated with Diabetic Foot Ulcer

Enhanced Expression of miR-34c in Peripheral Plasma Associated with Diabetic Foot Ulcer in Type 2 Diabetes Patients

Decreased expression of miR‐24 in peripheral plasma of type 2 diabetes mellitus patients associated with diabetic foot ulcer

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