The role of miR-150 regulates bone cell differentiation and function

Moussa, Fouad M.; Cook, Bryson; Sondag, Greg R; DeSanto, Matthew; Obri, Mark; McDermott, Scott E; Safadi, Fayez F.

doi:10.1016/j.bone.2020.115470

Cited by 19 publications

(19 citation statements)

References 44 publications

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“…The effect of miR-2335-5p, miR-101c-5p in osteogenic differentiation has never been reported. In addition, miR-150, sponged by lncRNA TCONS_00206,603, was proved to serve as a negative regulator for osteoblasts (Moussa et al, 2021), which was found to be upregulated in SOP patients in our study. However, we have not detected any downregulation of lncRNA TCONS_00206,603 in SOP patient.…”

Section: Discussionsupporting

confidence: 51%

Systematic Analysis of mRNAs and ncRNAs in BMSCs of Senile Osteoporosis Patients

Geng¹,

Chen²,

Chang³

et al. 2021

Front. Genet.

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Senile osteoporosis (SOP) is a worldwide age-related disease characterized by the loss of bone mass and decrease in bone strength. Bone mesenchymal stem cells (BMSCs) play an important role in the pathology of senile osteoporosis. Abnormal expression and regulation of non-coding RNA (ncRNA) are involved in a variety of human diseases. In the present study, we aimed to identify differentially expressed mRNAs and ncRNAs in senile osteoporosis patient-derived BMSCs via high-throughput transcriptome sequencing in combination with bioinformatics analysis. As a result, 415 mRNAs, 30 lncRNAs, 6 circRNAs and 27 miRNAs were found to be significantly changed in the senile osteoporosis group. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis were applied to analyze the function of differentially expressed mRNAs and ncRNAs. The circRNA–miRNA–mRNA regulatory network was constructed using the cytoHubba plugin based on the Cytoscape software. Interestingly, circRNA008876-miR-150-5p-mRNA was the sole predicted circRNA-miRNA-mRNA network. The differential expression profile of this ceRNA network was further verified by qRT-PCR. The biological function of this network was validated by overexpression and knockdown experiments. In conclusion, circRNA008876-miR-150-5p-mRNA could be an important ceRNA network involved in senile osteoporosis, which provides potential biomarkers and therapeutic targets for senile osteoporosis.

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Section: Discussionsupporting

confidence: 51%

Systematic Analysis of mRNAs and ncRNAs in BMSCs of Senile Osteoporosis Patients

Geng¹,

Chen²,

Chang³

et al. 2021

Front. Genet.

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“…micro-CT scanning was performed to evaluate bone phenotype using Bruker Sky1172 Skyscan (Kontich, Belgium). A total of 100 slices (1 mm) below the distal growth plate of the femurs was measured for 3D reconstruction and quantification of trabecular bone as described previously (56). The X-ray tube was operated at 96 kV and 65 μA using a 0.5 mm Al filter with a resolution of 7.93 μm pixels.…”

Section: Bone Phenotype Analysismentioning

confidence: 99%

MicroRNA-155 regulates osteogenesis and bone mass phenotype via targeting S1PR1 gene

Zheng

et al. 2022

Preprint

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MicroRNA-155 (miR155) is overexpressed in various inflammatory diseases and cancer, in which bone resorption and osteolysis are frequently observed. However, the role of miR155 on osteogenesis and bone mass phenotype is still unknown. Here, we report a low bone mass phenotype in the long bone of miR155-Tg mice compared with control mice. In contrast, miR155-KO mice showed a high bone mass phenotype. miR155-KO mice showed robust bone regeneration in the ectopic and orthotopic model, but miR155-Tg mice showed compromised bone regeneration compared with the control mice. Similarly, the osteogenic differentiation potential of bone marrow stromal stem cells (BMSCs) from miR155-KO mice was robust and miR155-Tg was compromised compared with that of control mice. Moreover, miR155 knockdown in BMSCs from control mice showed higher osteogenic differentiation potential, supporting the results from miR155-KO mice. TargetScan analysis predicted S1PR1 as a target gene of miR155, which was further confirmed by luciferase assay and miR155 knockdown. S1PR1 overexpression in BMSCs robustly promoted osteogenic differentiation without affecting cell viability and proliferation. Thus, miR155 showed a catabolic effect on osteogenesis and bone mass phenotype via interaction with the S1PR1 gene, suggesting inhibition of miR155 as a potential strategy for bone regeneration and bone defect healing.

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“…During the transition of pro-B to pre-B lymphocytes, miR-150 must normally be downregulated, since its target, the transcription factor c-Myb [65], is necessary for the development of B lymphocytes of the hematopoietic system [66], thus its upregulation inhibits c-Myb, which inhibits the transition of pro-B to pre-B lymphocytes [67]. In addition, a recent study reported that miR-150 is expressed in different tissues and its expression increases in bones, progressively with age, playing an important role in bone homeostasis, in the differentiation and function of osteoblasts, and osteoclasts, since miR-150 serves as a negative regulator of osteoblasts and a positive regulator of osteoclasts, regulating the expression of osteoactivin/glycoprotein nonmetastatic melanoma protein B (Osteoactivin/GPNMB) [68]. It has been reported that miR-150 was significantly upregulated in inflamed human pulps compared to healthy pulps.…”

Section: Mir-150mentioning

confidence: 99%

The Role of microRNAs in Pulp Inflammation

Muñoz‐Carrillo

Vázquez-Alcaraz

Vargas-Barbosa

et al. 2021

Cells

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The dental pulp can be affected by thermal, physical, chemical, and bacterial phenomena that stimulate the inflammatory response. The pulp tissue produces an immunological, cellular, and vascular reaction in an attempt to defend itself and resolve the affected tissue. The expression of different microRNAs during pulp inflammation has been previously documented. MicroRNAs (miRNAs) are endogenous small molecules involved in the transcription of genes that regulate the immune system and the inflammatory response. They are present in cellular and physiological functions, as well as in the pathogenesis of human diseases, becoming potential biomarkers for diagnosis, prognosis, monitoring, and safety. Previous studies have evidenced the different roles played by miRNAs in proinflammatory, anti-inflammatory, and immunological phenomena in the dental pulp, highlighting specific key functions of pulp pathology. This systematized review aims to provide an understanding of the role of the different microRNAs detected in the pulp and their effects on the expression of the different target genes that are involved during pulp inflammation.

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The role of miR-150 regulates bone cell differentiation and function

Cited by 19 publications

References 44 publications

Systematic Analysis of mRNAs and ncRNAs in BMSCs of Senile Osteoporosis Patients

Systematic Analysis of mRNAs and ncRNAs in BMSCs of Senile Osteoporosis Patients

MicroRNA-155 regulates osteogenesis and bone mass phenotype via targeting S1PR1 gene

The Role of microRNAs in Pulp Inflammation

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